Results 191 to 200 of about 55,956 (220)
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Chromosomes polymorphisms of Sichuan wheat cultivars displayed by ND-FISH landmarks
Cereal Research Communications, 2021Although fluorescence in situ hybridization (FISH) karyotypes of wheat chromosomes have already been widely investigated, more probes are needed to enrich them. Novel karyotype characteristics of 166 common wheat cultivars bred from the hometown of Chinese Spring were revealed using 11 oligonucleotide (oligo) probes derived from nine kinds of tandem ...
Hu, Zhenglei +8 more
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Genome, 2021
Thinopyrum elongatum is an important gene pool for wheat genetic improvement. However, the origins of the Thinopyrum genomes and the nature of the genus’ intraspecific relationships are still controversial. In this study, we used single-copy nuclear genes and non-denaturing fluorescence in situ hybridization (ND-FISH) to characterize genome ...
Dai, Yi +6 more
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Thinopyrum elongatum is an important gene pool for wheat genetic improvement. However, the origins of the Thinopyrum genomes and the nature of the genus’ intraspecific relationships are still controversial. In this study, we used single-copy nuclear genes and non-denaturing fluorescence in situ hybridization (ND-FISH) to characterize genome ...
Dai, Yi +6 more
openaire +2 more sources
Chromosomal detection of simple sequence repeats (SSRs) using nondenaturing FISH (ND-FISH)
Chromosoma, 2010Simple Sequence Repeats (SSRs) are known to be scattered and present in high number in eukaryotic genomes. We demonstrate that dye-labeled oligodeoxyribonucleotides with repeated mono-, di-, tri, or tetranucleotide motifs (15-20 nucleotides in length) have an unexpected ability to recognize SSR target sequences in non-denatured chromosomes. The results
Ángeles, Cuadrado, Nicolás, Jouve
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Journal of Genetics, 2014
Portugal constitutes the southwestern end limit of the distribution area of Scots pine in the world. The population of ‘Ribeira das Negras’ (‘Serra do Geres’; NW Portugal) has been considered potentially native and peripheral. This species has 24 chromosomes, hardly distinguishable because of their similar size and shape. Cytogenetic studies are scarce
IVO PAVIA +4 more
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Portugal constitutes the southwestern end limit of the distribution area of Scots pine in the world. The population of ‘Ribeira das Negras’ (‘Serra do Geres’; NW Portugal) has been considered potentially native and peripheral. This species has 24 chromosomes, hardly distinguishable because of their similar size and shape. Cytogenetic studies are scarce
IVO PAVIA +4 more
openaire +2 more sources
A simple and effective ND-FISH probe design for identifying barley (Hordeum vulgare) chromosomes
Genetic Resources and Crop Evolution, 2018Fluorescence in situ hybridization (FISH) has been widely used to analyze the physical mapping of chromosomes and study the chromosome rearrangements during evolution. The conventional FISH technique is time-consuming and labor-intensive as it involves many experimental steps including (1) PCR amplification of the target sequence, (2) recovery of the ...
Deyi Hu +7 more
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Chromosome Research, 2009
We report a new technique-nondenaturing FISH (ND-FISH)-for the rapid detection of plant telomeres without the need for prior denaturation of the chromosomes. In its development, two modified, synthetic oligonucleotides, 21 nt in length, fluorescently labelled at their 5' and 3' ends and complementary to either the cytidine-rich (C(3)TA(3)) or guanosine-
Angeles, Cuadrado +2 more
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We report a new technique-nondenaturing FISH (ND-FISH)-for the rapid detection of plant telomeres without the need for prior denaturation of the chromosomes. In its development, two modified, synthetic oligonucleotides, 21 nt in length, fluorescently labelled at their 5' and 3' ends and complementary to either the cytidine-rich (C(3)TA(3)) or guanosine-
Angeles, Cuadrado +2 more
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[Application of ND-FISH in amphibians].
Dong wu xue yan jiu = Zoological research, 2014The recently popularized non-denaturing fluorescence in situ hybridization (ND-FISH) is a new technique that is both quick and efficient, in part because denaturing of both of the probes and the chromosomes is unnecessary. Synthetic simple sequence repeats (SSRs) labeled with fluorescein are used as probes to detect SSR-enriched chromosome regions and ...
CHANG, Xiao-Ai, XIA, Yun, ZENG, Xiao-Mao
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