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New records of Caulerpa andamanensis and C. minuta (Bryopsidales, Chlorophyta) from Okinoerabujima Island, Ryukyu Archipelago, Japan

Phycological Research, EarlyView.
SUMMARY We identified 10 Caulerpa species collected from Okinoerabujima Island, Ryukyu Archipelago, Japan, using the tufA DNA barcode marker. Their tufA sequences showed 99.9–100% identity with tufA sequences available in GenBank. Among them, C. andamanensis and C.
Masahiro Suzuki, Rico Ohashi, Chisato Hasegawa, Mizuho Namba, Chika Kosugi, Ryuta Terada   +5 more
wiley   +1 more source

Black mangrove growth and root architecture in recycled glass sand: testing a new substrate for coastal restoration

Restoration Ecology, EarlyView.
As coastal regions experience accelerating land loss, artificial substrates may be useful in restoration efforts to replenish sediment and facilitate plant colonization. Recycled glass sand is a potential artificial substrate for marsh building due to its sustainability, availability, and similarity to natural substrates.
Kathryn H. Fronabarger, Alan J. Deogracias III, Kacey Lange, Elizabeth H. MacDougal, Bek X. L. Markel, Sunshine A. Van Bael, Emily C. Farrer   +6 more
wiley   +1 more source

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Application of nested PCR for diagnosis of histoplasmosis

Journal of Infection and Chemotherapy, 2013
Histoplasmosis is a fungal infection that, although not endemic in Japan, has seen a rise in the number of Japanese cases since the mid-1980s. Diagnosis of the disease is not straightforward, and the main method of detection, fungal culture (which has biosafety-related issues), is of low sensitivity in general.
Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Yukihiro Kaneko, Satoshi Yamagoe, Yoshitsugu Miyazaki, Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Satoshi Yamagoe   +9 more
openaire   +2 more sources

Nested PCR for the Detection of Cryptosporidium parvum

Parasitology Today, 1999
In their recent review, Morgan and Thompson1xMorgan, U.M. and Thompson, R.C.A. Parasitol. Today. 1998; 14: 241–245Abstract | Full Text | Full Text PDF | PubMed | Scopus (48)See all References1 described the pitfalls of using nested PCR for the detection of the diarrhoea-causing protozoan, Cryptosporidium parvum, in faecal samples or in waste or surface
C L, Gibbons, F M, Awad-El-Kariem
openaire   +2 more sources

Application of nested-PCR technique for the diagnosis of tuberculosis

Bulletin of Experimental Biology and Medicine, 2000
A test-system based on amplification of IS 986 fragment (nested-PCR) was developed for the detection of Mycobacterium tuberculosis and M. bovis in different biological samples. We constructed external primers and selected appropriate amplifications parameters (annealing temperatures for states I and II, the number of cycles for each amplification stage,
O I, Skotnikova, A Y, Sobolev, V V, Demkin, N P, Nikolaeva, E Y, Nosova, E L, Isaeva, A M, Moroz, V I, Litvinov   +7 more
openaire   +2 more sources

NESTED-PCR ASSAYS FOR DETECTION OF PHYTOPLASMAS IN STRAWBERRY

Acta Horticulturae, 1997
Flower and fruit virescence and phyllody are known to be associated with the presence of phytoplasmas in symptomatic tissues. During surveys in North and South Italy symptomatic and asymptomatic strawberry samples were examined by nested polymerase chain reaction (PCR) to verify the presence of phytoplasmas using 16S ribosomal DNA primers.
Bertaccini A., Vibio M., Pastore M., Recupero S., Guerrini S., Grimaldi D.   +5 more
openaire   +2 more sources

Multiplex nested PCR for Brazilian Alphavirus diagnosis

Transactions of the Royal Society of Tropical Medicine and Hygiene, 2004
A reverse-transcriptase PCR (RT-PCR) and a multiplex nested PCR were developed for the rapid detection and identification of 14 Brazilian alphaviruses. Using Alphavirus genus-specific primers in a RT-PCR, we obtained amplified products of 434 bp. Species-specific primers were selected and simultaneously tested in a multiplex nested PCR.
Roberta V M, Bronzoni, Marcos L, Moreli, Ana Cecília R, Cruz, Luiz Tadeu M, Figueiredo   +3 more
openaire   +2 more sources

Detection of Theileria orientalis in Iran by semi-nested PCR

Parasitology Research, 2011
In order to identify and differentiate Theileria orientalis in cattle which may be infected with Theileria annulata simultaneously, a semi-nested PCR was performed. Thus, 160 blood samples were collected from apparently healthy native cattle in Golestan province of northern Iran, during 2009 to 2011. The Tbs-S/Tbs-A primer set derived from the 18S rRNA
Peyman, Ghaemi, Nasser, Hoghooghi-Rad, Parviz, Shayan, Brigitte, Eckert   +3 more
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A rapid hemi-nested PCR for HTLV-I detection

Journal of Clinical Virology, 2003
A hemi-nested PCR approach was adopted to detect HTLV-1 infection in clinical samples of peripheral blood mononuclear cells (PBMCs) from subjects with positive or indeterminate serological results. Our results showed that the hemi-nested PCR quickly solved the diagnostic query, detecting the presence of proviral HTLV-1 DNA in two of the 252 patients ...
Davide Gibellini, Paola Monari, Monica Sassi, Maria Carla Re   +3 more
openaire   +2 more sources

Diagnosis of Oropouche virus infection by RT‐nested‐PCR

Journal of Medical Virology, 2001
AbstractUsing the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients.
Marcos Lázaro, Moreli, Victor Hugo, Aquino, Ana Cecília R, Cruz, Luiz Tadeu M, Figueiredo   +3 more
openaire   +2 more sources