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Application of nested-PCR technique for the diagnosis of tuberculosis

Bulletin of Experimental Biology and Medicine, 2000
A test-system based on amplification of IS 986 fragment (nested-PCR) was developed for the detection of Mycobacterium tuberculosis and M. bovis in different biological samples. We constructed external primers and selected appropriate amplifications parameters (annealing temperatures for states I and II, the number of cycles for each amplification stage,
O I, Skotnikova   +7 more
openaire   +2 more sources

Detection of HPV Transcripts by Nested RT-PCR

2005
Human papillomaviruses (HPVs) are etiologic for the development of cervical cancer and its precursor lesions, cervical intraepithelial neoplasia (CIN). Nearly all cervical carcinomas (CaCx) harbor HPV DNA, but the presence of HPV alone is not indicative of the future development of neoplasia.
Christine, Mant   +2 more
openaire   +2 more sources

Nested PCR and Multiplex Nested PCR

2004
David Tarragó‐Asensio   +1 more
openaire   +2 more sources

Nested PCR

2007
Opisana je metoda nested PCR koja se sastoji od dvije reakcije PCR u kojima se koriste dva različita para početnica.
openaire  

Nested PCR

2011
BONIN, Serena, DOTTI, ISABELLA
openaire   +3 more sources

Nested PCR

2015
Hans-Joachim Müller   +1 more
openaire   +1 more source

Nested PCR ve Kullanım Alanları

2013
Nested PCR, PCR tekniğinin hassasiyetini artırmak için geliştirilen en popular yöntemlerden biridir. İstenilen DNA dizisinin bulunup çoğaltılmasında hassasiyeti 104 kat artırdığı tahmin edilmektedir. Bu reaksiyon mekanizmasında birbirini takip eden iki polimeraz zincir reaksiyonunu içermektedir. İlk amplifikasyonda hedef DNA’nın dış bölgesine özgün iki
SEVİNDİK, Emre, ABACI, Z. Tuğba
openaire   +1 more source

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