Results 141 to 150 of about 97,206 (312)

Nested polymerase chain reaction for early diagnosis of typhoid fever.

open access: yesMymensingh medical journal : MMJ, 2012
Typhoid fever, caused by Salmonella typhi, is an important cause of morbidity and mortality in many developing countries. A rapid and sensitive method for the detection of S. typhi is essential for early diagnosis. This was a study to prospectively evaluate the sensitivity and specificity of nested polymerase chain reaction (PCR) to identify the S ...
S, Sultana   +10 more
openaire   +1 more source

Regulation of PHOX2B gene expression by the long non‐coding natural antisense RNA PHOX2B‐AS1

open access: yesThe FEBS Journal, EarlyView.
PHOX2B is a transcription factor essential for autonomic nervous system development. We identify and characterize PHOX2B‐AS1, a human long non‐coding antisense transcript at the PHOX2B locus, along with its murine counterpart. Our findings reveal bidirectional transcription and reciprocal regulation: PHOX2B activates PHOX2B‐AS1, whereas PHOX2B‐AS1 ...
Simona Di Lascio   +12 more
wiley   +1 more source

Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection. [PDF]

open access: yesIntern Med, 2021
Minemura M   +10 more
europepmc   +1 more source

Population‐Based Molecular Surveillance of Hepatitis B Virus Genotypes in the Goto Islands, Japan: A Three Decade Study (1977–2008) of 916 Carriers

open access: yesHepatology Research, EarlyView.
ABSTRACT Aims Understanding the molecular characteristics and genotype distribution of hepatitis B virus (HBV) is essential to elucidate intra and inter community transmission routes, identify cluster cases, and assess clinical implications based on genotype‐specific virulence.
Ko Ko   +10 more
wiley   +1 more source

Making Molecular Diagnostics Faster

open access: yesInternational Journal of Laboratory Hematology, EarlyView.
ABSTRACT Background Over the past 40 years, molecular diagnostic methods have evolved from multi‐step, time‐consuming protocols towards either rapid targeted tests or expansive, massively parallel testing. Aims Here we consider the speed limits of targeted molecular diagnostics, considering the three sequential required steps: nucleic acid preparation,
Carl T. Wittwer   +5 more
wiley   +1 more source

Variation in Microbiome Composition and Faecal Metabolites Are Associated With Differential Susceptibility to DSS‐Induced Colitis

open access: yesImmunology, EarlyView.
C57BL/6 mice from Charles River Laboratories (CR) have enhanced mucosal immune action relative to C57BL/6 mice from Jackson Laboratories (JAX). CR mice are more susceptible to severe DSS‐induced colitis than JAX mice. Co‐housing transfers colitis susceptibility from CR mice to JAX mice.
Jessica M. Till   +8 more
wiley   +1 more source

‘It's not much, but it's honest work’: The status of environmental DNA analyses of fish biodiversity in southern Africa

open access: yesJournal of Fish Biology, EarlyView.
Abstract Environmental DNA (eDNA) biodiversity surveys have the power to transform the detection of species in natural environments, which is crucial for the conservation and management of freshwater, estuarine and marine environments. Globally, eDNA‐based analyses have increased significantly, with fishes being the most widely studied aquatic ...
Sophie von der Heyden
wiley   +1 more source

Morphological and molecular evidence reveals a new species of Characidium from the Ucayali‐Urubamba Piedmont, Peru, and novel molecular clades are proposed within the genus

open access: yesJournal of Fish Biology, EarlyView.
Abstract A new species of Characidium is described from the Urubamba River basin, within the Ucayali‐Urubamba Piedmont ecoregion, Peru. The new species can be readily distinguished from all congeners, except Characidium cacah, Characidium chicoi, Characidium helmeri, Characidium mirim, Characidium nana, Characidium nupelia, Characidium sterbai ...
Leonardo Oliveira‐Silva   +6 more
wiley   +1 more source

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