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DETECTION OF PLASMODIUM INFECTION BY NESTED POLYMERASE CHAIN REACTION ASSAY v3

, 2017
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Nested Polymerase Chain Reaction (PCR)

Cold Spring Harbor Protocols, 2019
Nested polymerase chain reaction (PCR) is used in situations in which it is necessary to increase the sensitivity and/or specificity of PCR, for example, when amplifying a particular member of a polymorphic gene family or when amplifying a cDNA copy of an mRNA present at very low abundance in a clinical specimen containing a heterogeneous population of
Michael R, Green, Joseph, Sambrook
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Nested Polymerase Chain Reaction and Cutaneous Tuberculosis

The American Journal of Dermatopathology, 2019
Abstract: The role of Mycobacterium tuberculosis in the etiology and pathogenesis of cutaneous tuberculosis is controversial because of the difficulties associated with demonstrating the presence of these mycobacteria in tuberculid cutaneous lesions by routinely available microbiological and histological techniques. In this study, we aimed to
Carmen, Maldonado-Bernal, Alberto, Ramos-Garibay, Nora, Rios-Sarabia, Héctor, Serrano, Manuel, Carrera, Gisela, Navarrete-Franco, Fermín, Jurado-Santacruz, Armando, Isibasi   +7 more
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Urine Nested Polymerase Chain Reaction in Neonatal Septicemia

Journal of Tropical Pediatrics, 2015
This cross-sectional study was done to evaluate diagnostic efficacy of urine nested polymerase chain reaction (PCR) using broad-range 16SrDNA PCR-based amplification, followed by restriction analysis and sequencing in neonatal septicemia. The study included 50 babies; 48% had vaginal delivery, 46% were preterm, 20% had a history of prolonged rupture of
B K, Das, Shipra, Suri, Gopal, Nath, Rajniti, Prasad   +3 more
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Nested polymerase chain reaction for detection of pathogenic leptospires

Canadian Journal of Microbiology, 2006
Leptospirosis is a widespread zoonosis caused by pathogenic members of the genus Leptospira that has a great impact on human and veterinary public health. Early diagnosis of leptospirosis is important because severe lepto spiral infection can have a fulminant course.
Sandra Denize Dorneles, Jouglard, Simone, Simionatto, Fabiana Kommling, Seixas, Fernanda Lima, Nassi, Odir Antônio, Dellagostin   +4 more
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A nested polymerase chain reaction assay to differentiate pestiviruses

Virus Research, 1995
Viruses that comprise the Pestivirus genus cause significant losses to the livestock industry. Based on sequence analysis, currently 4 distinct genotypes are identified of which 3 infect cattle and sheep. Distinguishing between bovine and ovine isolates by serological tests has often been difficult because of a high degree of cross reactivity.
D G, Sullivan, R K, Akkina
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Diagnosis of Rabies by Polymerase Chain Reaction with Nested Primers

Journal of Infectious Diseases, 1993
A simple, sensitive, and specific polymerase chain reaction (PCR) protocol for detection of rabies virus is described. The process consists of sample preparation, reverse transcription, two-step DNA amplification, and detection of the amplified product.
N, Kamolvarin, T, Tirawatnpong, R, Rattanasiwamoke, S, Tirawatnpong, T, Panpanich, T, Hemachudha   +5 more
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Identification of Listeria species by a semi-nested polymerase chain reaction

Research in Microbiology, 1996
Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.
MANZANO, Marisa, COCOLIN L, FERRONI P, GASPARINI, Vinicio, NARDUZZI D, CANTONI C, COMI, Giuseppe   +6 more
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Detection of human parvovirus B19 DNA by nested polymerase chain reaction

Obstetrics & Gynecology, 1995
We describe the development of a nested polymerase chain reaction (PCR) technique used to detect human parvovirus B19 DNA. It was performed in a two-step reaction, first with a pair of outer primers, then with a pair of inner (ie, nested) primers. Primer sequences were selected in the VP1 gene, corresponding to the capsid protein, of human parvovirus ...
Y, Yamakawa, H, Oka, S, Hori, T, Arai, R, Izumi   +4 more
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Detection of Legionella pneumophila in wastewater by nested polymerase chain reaction

Research in Microbiology, 1997
The study of Legionella in treated wastewater acquires special importance when this water is used in irrigation by spray, as Legionella is transmitted via the inhalation of aerosols and may consequently represent a health risk. In this study, we applied polymerase chain reaction (PCR) amplification as an alternative method to plate culture for ...
V, Catalan, F, Garcia, C, Moreno, M J, Vila, D, Apraiz   +4 more
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