Results 241 to 250 of about 49,900 (269)

Detection of Plasmodium falciparum in Human Blood by a Nested Polymerase Chain Reaction

The American Journal of Tropical Medicine and Hygiene, 1994
We have developed a simple method for direct detection of Plasmodium falciparum parasites in infected human blood using a nested polymerase chain reaction. Whole blood (10 microliters) was obtained by finger puncture and suspended in a microcentrifuge tube containing phosphate-buffered saline. For removal of components that might inhibit the PCR, blood
M, Arai, C, Mizukoshi, F, Kubochi, T, Kakutani, Y, Wataya   +4 more
openaire   +2 more sources

Monitoring the therapy of pulmonary tuberculosis by nested polymerase chain reaction assay

Journal of Infection, 1997
Monthly clinical and microbiological parameters (by sputum smear, culture and PCR assay), of 50 patients with documented pulmonary tuberculosis and on anti-tuberculosis therapy, were monitored over a period of 18 months. PCR converters (70%) who had PCR conversion before the sixth month of treatment did not have clinical or microbiological evidence of ...
Yuen, KY, Chan, CM, Ng, MH, Ho, PL, Chan, KS   +4 more
openaire   +4 more sources

Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses

Journal of Veterinary Medicine, Series B, 2002
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
M, Elschner, J, Prudlo, H, Hotzel, P, Otto, K, Sachse   +4 more
openaire   +2 more sources

Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis

Neurology, 2005
The authors examined the usefulness of nested PCR (N-PCR) to detect Mycobacterium tuberculosis (MTB) DNA in CSF for assessing the clinical course of tuberculous meningitis (TBM). N-PCR successfully detected MTB DNA in all nine CSF samples from patients with suspected TBM.
T, Takahashi, T, Nakayama, M, Tamura, K, Ogawa, H, Tsuda, A, Morita, M, Hara, M, Togo, H, Shiota, Y, Suzuki, M, Minami, H, Ishikawa, K, Miki, E, Shikata, S, Takahashi, T, Kuragano, K, Matsumoto, S, Sawada, T, Mizutani   +18 more
openaire   +2 more sources

Competitive nested polymerase chain reaction for quantification of humanMDR1 gene expression

Journal of Cancer Research and Clinical Oncology, 1994
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Multidrug resistance (MDR) describes the simultaneous expression of cellular resistance to a wide range of structurally and functionally unrelated drugs.
F, Grünebach, E U, Griese, K, Schumacher   +2 more
openaire   +2 more sources

Diagnosis of Herpetic Keratoconjunctivitis by Nested Polymerase Chain Reaction in Human Tear Film

European Journal of Clinical Microbiology & Infectious Diseases, 1998
A study was performed to evaluate nested PCR (nPCR) versus viral cultures as method and tear film versus corneal scrapings as specimen in the diagnosis of viral keratoconjunctivitis. Tear film specimens were taken from both eyes and corneal scrapings from the affected eye only in 17 patients with suspected viral keratoconjunctivitis.
F, Hidalgo, S, Melón, M, de Oña, V, Do Santos, A, Martínez, R, Cimadevilla, M, Rodríguez   +6 more
openaire   +2 more sources

Rapid Simple and Nested Polymerase Chain Reaction for the Diagnosis of Pneumocystis carinii pneumonia

Scandinavian Journal of Infectious Diseases, 1995
We have developed a rapid and easy extraction procedure for polymerase chain reaction (PCR) protocols. Using this simplified step, we evaluated the sensitivity and the specificity of a simple PCR using the primers of Wakefield et al, and of a nested PCR, using new internal primers selected by us, in a total of 89 bronochoalveolar lavage (BAL) fluid ...
Moonens, Françoise, Liesnard, Corinne, Brancart, Francoise, Van Vooren, Jean-Paul, Schoutens Serruys, Elisabeth   +4 more
openaire   +3 more sources

Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology, 1999
Nested polymerase chain reaction (PCR) was applied to identify the serotypes of Rickettsia tsutsugamushi isolated from patients. The primers used for PCR were based on the nucleotide sequences encoding a 56 kDa antigen of rickettsiae. Comparing to the conventional immunofluorescence assay (IFA), which displays a considerable degree of cross-reactivity ...
G J, Shieh, H L, Chen, H Y, Chen, G R, Wang, C B, Horng   +4 more
openaire   +1 more source

[Detection of human cytomegalovirus DNA by nested polymerase chain reaction].

Rinsho byori. The Japanese journal of clinical pathology, 1995
Detection of human cytomegalovirus (CMV) DNA from peripheral blood and urine specimens taken from renal transplant and bone marrow transplant recipients was examined by using nested polymerase chain reaction (PCR). Results by the PCR were compared with those of healthy subjects.
K, Yamashita, N, Otsuka, Y, Nakadoori, S, Kagawa, A, Matsuoka   +4 more
openaire   +1 more source

High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction

Molecular and Biochemical Parasitology, 1993
Georges Snounou, S Viriyakosol, W Jarra   +2 more
exaly