Results 231 to 240 of about 49,900 (269)

Reliability of nested polymerase chain reaction in the diagnosis of bacterial endophthalmitis

American Journal of Ophthalmology, 2002
To test the utility of DNA Taq polymerase (Taq) treated with 8-methoxypsoralen with ultraviolet irradiation and Taq treated with restriction endonuclease in a nested polymerase chain reaction test for the diagnosis of bacterial endophthalmitis.Prospective, comparative study.
Savitri, Sharma, Debashish, Das, Raj, Anand, Taraprasad, Das, Chitra, Kannabiran   +4 more
openaire   +2 more sources

Nested polymerase chain reaction in the diagnosis of congenital cytomegalovirus infection

The Indian Journal of Pediatrics, 2001
Human cytomegalovirus infection is highly prevalent in Indian population. It is the commonest congenitally acquired infection causing various anomalies. The diagnosis of infection in neonates is difficult as IgM may not be detected in all cases. The polymerase chain reaction is reported as alternative and better option in these patients. However, there
R, Sivakumar, N, Singh, S, Singh
openaire   +2 more sources

Quantitation of polyomavirus DNA by a competitive nested polymerase chain reaction

Journal of Virological Methods, 2000
A new method to quantitate small amounts of DNA in clinical specimens is described. The method, a nested competitive polymerase chain reaction (ncPCR), is able to quantitate between 10 and 10(6) copies per tube of polyomavirus DNA and shows good reproducibility when clinical samples are analysed. Throughout the whole procedure, an internal standard (IS)
C. G. Fedele, A. Avellon, CIARDI, Maria Rosa, DELIA, Salvatore, A. Tenorio   +4 more
openaire   +3 more sources

Detection of feline immunodeficiency virus by a nested polymerase chain reaction

Journal of Virological Methods, 1992
A specific and sensitive polymerase chain reaction (PCR) procedure for the detection of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMC) was developed. PBMC from both blood samples and cultures were digested by proteinase K in a lysis buffer, and after heat inactivation of the proteinase, the resultant material was used ...
E, Rimstad, K, Ueland
openaire   +2 more sources

Sensitive detection of canine parvovirus DNA by the nested polymerase chain reaction

Veterinary Microbiology, 1994
A polymerase chain reaction (PCR) for the detection of canine parvovirus (CPV) was developed. To increase the sensitivity and specificity of the reaction, the nested PCR with a double-nested primer pair (inner primer pair) was designed. The sequences of the PCR primer pairs were selected from the conserved region in the CPV VP1/VP2 gene.
T, Hirasawa, T, Kaneshige, K, Mikazuki
openaire   +2 more sources

Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction Targeting 47kDa Gene for the Diagnosis of Scrub Typhus

Indian Journal of Medical Microbiology, 2019
Scrub typhus is a zoonotic infection caused by Orientia tsutsugamushi which is transmitted by Leptotrombidium mites. The disease manifests as a mild-to-severe illness with non-specific clinical symptoms. Rapid diagnosis and prompt treatment are essential for patient management.
John Antony Jude Prakash, Thangam Menon
exaly   +3 more sources

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples

Journal of Clinical Microbiology, 1993
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in this study showed excellent specificity, sensitivity, and agreement with the conventional methods for 417 clinical samples, indicating a contribution to the
Y, Miyazaki, H, Koga, S, Kohno, M, Kaku
openaire   +2 more sources

[Nested coamplification polymerase chain reaction].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2002
To develop a simple and reliable method for intensifying the hybridization signals of gene chips.The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.With the use of EDTA and another FAM-labeled probe, the hybridization ...
Qingjie, Xia, Sizhong, Zhang, Yuan, Yang, Jun, Wang, Xiujie, Wang, Yongxin, Ma, Yan, Sun   +6 more
openaire   +1 more source

Detection of feline herpesvirus 1 DNA by the nested polymerase chain reaction

Veterinary Microbiology, 1996
The thymidine kinase region of feline herpesvirus 1 (FHV 1) genome in ocular/nasal swabs from cats with clinical manifestations of upper respiratory disease was amplified by nested polymerase chain reaction (nested PCR). Two primer pairs were prepared for nested PCR. FHV 1 DNA in ocular/nasal swabs was extracted using instaGene-DNA purification matrix.
M, Hara, M, Fukuyama, Y, Suzuki, S, Kisikawa, T, Ikeda, A, Kiuchi, K, Tabuchi   +6 more
openaire   +2 more sources

Detection of human parvovirus B19 DNA by nested polymerase chain reaction

Obstetrics & Gynecology, 1995
We describe the development of a nested polymerase chain reaction (PCR) technique used to detect human parvovirus B19 DNA. It was performed in a two-step reaction, first with a pair of outer primers, then with a pair of inner (ie, nested) primers. Primer sequences were selected in the VP1 gene, corresponding to the capsid protein, of human parvovirus ...
Y, Yamakawa, H, Oka, S, Hori, T, Arai, R, Izumi   +4 more
openaire   +2 more sources