Results 21 to 30 of about 97,206 (312)

Immunological diagnosis as an adjunctive tool for an early diagnosis of tuberculous meningitis of an immune competent child in a low tuberculosis endemic country: A case report [PDF]

, 2017
Background: Pediatric tuberculous meningitis is a highly morbid, often fatal disease. Its prompt diagnosis and treat - ment saves lives, in fact delays in the initiation of therapy have been associated with high mortality rates. Case presentation:
Ajassa, Camilla, Caraffa, Emanuela, Ciardi, Rosa Maria, Colistra, Davide, DI BIASI, Claudio, Lichtner, Miriam, Mancarella, Cristina, Mascia, Claudia, Mastroianni, Claudio Maria, Mengoni, Fabio, Paglia, Maria Grazia, Vita, Serena, Vullo, Vincenzo   +12 more
core   +4 more sources

Development of Nested Polymerase Chain Reaction for Detection of Rabies

Journal of Physics: Conference Series, 2022
Abstract Rabies is an acute progressive and zoonotic disease which is caused by the rabies virus. Rabies virus is the prototype virus belonging to the genus lyssavirus in the family Rhabdoviridae of the order Mononegavirales. The aim of this study was to develop a nested reverse-transcriptase polymerase chain reaction (nRTPCR) for the ...
Fazilah Muhamad Alwi, Musherah Khusaini, Ag. Muhammad Sagaf Abu Bakar, Ainol Azifa Mohd Faik   +3 more
openaire   +1 more source

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Iranian Journal of Public Health, 2022
Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis.
Gita Alizadeh, Mojgan Aryaeipour, Mehdi Mohebali, Gholam Reza Mowlavi, Vahid Raissi, Mohammad Bagher Rokni   +5 more
doaj   +1 more source

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh [PDF]

Veterinary World, 2021
Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T.
Tanjila Hasan, Abdul Mannan, Delower Hossain, Azizunnesa Rekha, Md. Monir Hossan, Mohammad Abdul Alim, AHM Musleh Uddin   +6 more
doaj   +1 more source

طراحی و توسعه‌ی Nested PCR سه‌گانه جهت تشخیص هم‌زمان Plasmodium، Babesia و Toxoplasma در فرایند انتقال خون [PDF]

مجله دانشکده پزشکی اصفهان, 2021
مقدمه: تشخیص و رفع عفونت‌های قابل انتقال از طریق انتقال خون از اهمیت به‌سزایی برخوردار می‌باشند. هدف از انجام این مطالعه، تشخیص Plasmodium، Toxoplasma و Babesia به طور هم‌زمان در نمونه‌های خون بود.روش‌ها: این مطالعه از نوع تجربی و توسعه‌ی روش Triplex ...
عباسعلی اسکندریان, علیرضا نجفی راد   +1 more
doaj   +1 more source

Discrepancies in the Diagnosis of Congenital <i>Toxoplasma gondii</i> Infection Between B1 Gene Semi-Nested Polymerase Chain Reaction and Serological Analyses. [PDF]

Microorganisms
Uchida A, Tanimura K, Shirai K, Ashina M, Fujioka K, Morioka I, Sasai M, Yamamoto M, Yamada H.   +8 more
europepmc   +3 more sources

Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]

, 2016
Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M, Castellani, F, Lleo, M. M., Lucidi, Cristina, Merli, Manuela   +4 more
core   +1 more source

Qualitative and quantitative polymerase chain reaction monitoring of minimal residual disease in relapsed chronic lymphocytic leukemia: early assessment can predict long-term outcome after reduced intensity allogeneic transplantation

Haematologica, 2009
Background The graft-versus-leukemia effect is able to induce clinical responses in patients with chronic lymphocytic leukemia treated with a reduced intensity conditioning regimen, followed by allogeneic stem cell transplantation.
Lucia Farina, Cristiana Carniti, Anna Dodero, Antonio Vendramin, Anna Raganato, Francesco Spina, Francesca Patriarca, Franco Narni, Fabio Benedetti, Attilio Olivieri, Paolo Corradini   +10 more
doaj   +1 more source

Detection of Legionella pneumophila Using a Nested Polymerase Chain Reaction

Journal of the Japanese Association for Infectious Diseases, 1992
We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively.
Futoshi HIGA, Michio KOIDE, Takashi SHINZATO, Atsushi NAKAMOTO, Takayuki MIYARA, Michihiro GAJA, Masao TATEYAMA, Isoko OWAN, Nobuchika KUSANO, Keizo KITSUKAWA, Yoshiteru SHIGENO, Atsushi SAITO   +11 more
openaire   +3 more sources

A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

Notulae Scientia Biologicae, 2011
This work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide ...
Shasha WEI, Zhirui DENG, Liping YIN, Jianping YI, Renqi WU, Qin CHEN   +5 more
doaj   +3 more sources