Results 31 to 40 of about 49,900 (269)

Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens

open access: yesBrazilian Journal of Infectious Diseases, 2014
Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop
Taha Roodbar Shojaei   +7 more
doaj   +1 more source

Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens [PDF]

open access: yes, 1994
Discrimination of pathogenic and nonpathogenic Entamoeba histolytica is of great clinical importance. A simple and rapid DNA extraction method that can be used directly with stool specimens was developed without the need for prior cultivation of the ...
Katzwinkel-Wladarsch, S.   +5 more
core   +1 more source

Utility of PCR in the Diagnosis of Female Genital Tuberculosis [PDF]

open access: yesJournal of Microbiology and Infectious Diseases, 2014
Genital tuberculosis (GTB) is one of the major causes of infertility among females. Since the infection is asymptomatic in most of the cases, it makes the clinical diagnosis quite difficult. We report a case of a 24 years old female with infertility that
Gill Manmeet Kaur, Aggarwal Aruna
doaj   +1 more source

A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens [PDF]

open access: yesClinical Microbiology and Infection, 1997
OBJECTIVE: Because presently used methods for diagnosis of Legionella pneumonia lack sufficient sensitivity and sometimes specificity and rapidity, the detection of Legionella spp. by amplification of nucleic acids might be valuable. However, performing polymerase chain reaction (PCR) on clinical samples such as sputum is difficult because of the ...
Bernander, Sverker   +3 more
openaire   +2 more sources

Rapid detection of Salmonella in field samples by nested polymerase chain reaction [PDF]

open access: yesLetters in Applied Microbiology, 1999
A simple and universal protocol for the rapid detection of Salmonella spp. in various samples using nested polymerase chain reaction (PCR) was developed. The protocol takes advantage of the rapid purification and concentration of Salmonella by centrifugation onto a layer of 60% sucrose solution.
I, Rychlík   +5 more
openaire   +2 more sources

Analysis, comparison, and significance of nested polymerase chain reaction with conventional methods for the diagnosis of pulmonary and extrapulmonary tuberculosis among the rural population of North India

open access: yesCHRISMED Journal of Health and Research, 2019
Introduction: Tuberculosis (TB) is one of the most common infectious diseases among the Indian population, especially in rural scenario. It affects young people in majority. Living at slum areas and poverty are predisposing etiological factors.
Amit Singh, Seema Dayal
doaj   +1 more source

Diagnosis of Human Immunodeficiency Virus Infection by Polymerase Chain Reaction

open access: yes, 1992
Infection by the human immunodeficiency virus (HIV) is routinely diagnosed by the identification of specific antibodies in serum. In some cases, as in primary infection, HIV can be detected prior to seroconversion by the polymerase chain reaction (PCR ...
Albert, J   +3 more
core   +1 more source

Primers used in the nested polymerase chain reaction.

open access: yes, 2014
Primers used in the nested polymerase chain reaction.
Yves Guiot (189842)   +7 more
core   +1 more source

Identification of human papillomavirus DNA in cutaneous lesions of Cowden syndrome [PDF]

open access: yes, 2003
Background: Cowden syndrome (CS) or multiple hamartoma syndrome is a cancer-associated genodermatosis inherited in an autosomal dominant pattern. One of the diagnostic criteria is facial papules which are felt to be trichilemmomas, benign hair follicle ...
Schaller, Jörg   +4 more
core   +1 more source

Detection of Legionella pneumophila Using a Nested Polymerase Chain Reaction

open access: yesJournal of the Japanese Association for Infectious Diseases, 1992
We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively.
HIGA, Futoshi   +11 more
openaire   +3 more sources

Home - About - Disclaimer - Privacy