Results 21 to 30 of about 49,900 (269)

طراحی و توسعه‌ی Nested PCR سه‌گانه جهت تشخیص هم‌زمان Plasmodium، Babesia و Toxoplasma در فرایند انتقال خون [PDF]

مجله دانشکده پزشکی اصفهان, 2021
مقدمه: تشخیص و رفع عفونت‌های قابل انتقال از طریق انتقال خون از اهمیت به‌سزایی برخوردار می‌باشند. هدف از انجام این مطالعه، تشخیص Plasmodium، Toxoplasma و Babesia به طور هم‌زمان در نمونه‌های خون بود.روش‌ها: این مطالعه از نوع تجربی و توسعه‌ی روش Triplex ...
عباسعلی اسکندریان, علیرضا نجفی راد   +1 more
doaj   +1 more source

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller, Ganesh Srinivasamoorthy, Venkatachalam Udhayakumar, John W. Barnwell, Leopoldo Villegas, S. Patrick Kachur, Demas, Allison, Kissinger, Jessica C, Villegas, Leopoldo, Abdunoor M. Kabanywanyi, Jessica C. Kissinger, Deborah Sumari, Escalante, Ananias A, Lucchi, Naomi W, Udhayakumar, Venkatachalam, Sumari, Deborah, Peterson, David S, Ananias A. Escalante, Naomi W. Lucchi, Jeremy DeBarry, DeBarry, Jeremy, David S. Peterson, Oberstaller, Jenna, Allison Demas, Srinivasamoorthy, Ganesh, Kachur, S Patrick, Kabanywanyi, Abdunoor M, Barnwell, John W   +27 more
core   +1 more source

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh [PDF]

Veterinary World, 2021
Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T.
Tanjila Hasan, Abdul Mannan, Delower Hossain, Azizunnesa Rekha, Md. Monir Hossan, Mohammad Abdul Alim, AHM Musleh Uddin   +6 more
doaj   +1 more source

Characterization of a nested polymerase chain reaction assay for detection of parvovirus B19 [PDF]

Journal of Clinical Microbiology, 1993
The characterization and application of a nested polymerase chain reaction (PCR) assay for the detection of human parvovirus B19 DNA is described. The assay was evaluated with 149 diagnostic serum samples (collected up to 150 days after the onset of symptoms) previously tested by dot blot hybridization for B19 DNA and by class-specific capture ...
G, Patou, D, Pillay, S, Myint, J, Pattison   +3 more
openaire   +2 more sources

Qualitative and quantitative polymerase chain reaction monitoring of minimal residual disease in relapsed chronic lymphocytic leukemia: early assessment can predict long-term outcome after reduced intensity allogeneic transplantation

Haematologica, 2009
Background The graft-versus-leukemia effect is able to induce clinical responses in patients with chronic lymphocytic leukemia treated with a reduced intensity conditioning regimen, followed by allogeneic stem cell transplantation.
Lucia Farina, Cristiana Carniti, Anna Dodero, Antonio Vendramin, Anna Raganato, Francesco Spina, Francesca Patriarca, Franco Narni, Fabio Benedetti, Attilio Olivieri, Paolo Corradini   +10 more
doaj   +1 more source

Clinical usefulness of the nested polymerase chain reaction in the diagnosis of extrapulmonary tuberculosis [PDF]

Salud Pública de México, 2009
To evaluate the effectiveness of nested polymerase chain reaction (PCR) for diagnosis of extrapulmonary tuberculosis (ETB), as well as the impact of PCR results on clinical management.We conducted a study of nested PCR tests in 45 patients and a review of patient hospital files, calculating sensitivity, specificity, positive predictive value (PPV), and
Guadalupe García-Elorriaga, Carlos Gracida-Osorno, Guadalupe Carrillo-Montes, César González-Bonilla   +3 more
openaire   +3 more sources

A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

Notulae Scientia Biologicae, 2011
This work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide ...
Shasha WEI, Zhirui DENG, Liping YIN, Jianping YI, Renqi WU, Qin CHEN   +5 more
doaj   +3 more sources

Simultaneous monitoring of CMV and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up

Clinics, 2011
OBJECTIVE: The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing ...
Fernanda Aparecida Costa, Marcelo Naoki Soki, Paula Durante Andrade, Sandra Helena Alves Bonon, Ronaldo Luis Thomasini, Ana Maria Sampaio, Marcelo de Carvalho Ramos, Claudio Lúcio Rossi, Teresa Cristina Cavalcanti, Ilka de Fatima Boin, Marília Leonard, Luiz Sérgio Leonard, Raquel Bello Stucchi, Sandra Cecília Botelho Costa   +13 more
doaj   +1 more source

The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019

Microorganisms, 2020
Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales.
Seong Sik Jang, Ji Yeong Noh, Van Thi Lo, Yong Gun Choi, Sun-Woo Yoon, Dae Gwin Jeong, Hye Kwon Kim   +6 more
doaj   +1 more source

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases, 2011
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD   +4 more
doaj   +1 more source