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Frontiers in Bioscience-Landmark, 2022 Background: Several commercial surrogate Virus Neutralization Tests (sVNTs) have been developed in the last year. Neutralizing anti-SARS-CoV-2 antibodies through interaction with Spike protein Receptor Binding Domain (S-RBD) can block the virus from ...
Massimo Pieri +14 more
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Microbiology Spectrum, 2023 Neutralizing antibodies (nAbs) are considered a valuable marker for measuring humoral immunity against SARS-CoV-2. However, live-virus neutralization tests (NTs) require high-biosafety-level laboratories and are time-consuming. Therefore, surrogate virus
Marianne Graninger +14 more
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Molecular Classification of Enteroviruses Not Identified by Neutralization Tests
Emerging Infectious Diseases, 2002 We isolated six viruses from patients diagnosed with aseptic meningitis or hand, foot, and mouth disease. The cytopathic effect of these viruses on cultured cells was like that of enteroviruses.
Hideyuki Kubo +2 more
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Neutralization tests with varicella-zoster virus [PDF]
Journal of Hygiene, 1969 SUMMARYA technique for neutralization tests using varicella-zoster virus propagated in primary human thyroid cells is described. The level of neutralizing antibody following chickenpox does not usually exceed a titre of 1/40 and in adults many years after infection it may be very low.
D. G. Shaw, Anne E. Caunt
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Viral infection neutralization tests: A focus on severe acute respiratory syndrome-coronavirus-2 with implications for convalescent plasma therapy. [PDF]
Rev Med Virol, 2021 Viral neutralization tests (VNTs) have long been considered old‐fashioned tricks in the armamentarium of fundamental virology, with laboratory implementation for a limited array of viruses only. Nevertheless, they represent the most reliable surrogate of
Focosi D +3 more
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Vero microcultures for adenovirus neutralization tests [PDF]
Journal of Clinical Microbiology, 1978 A microculture neutralization test is described for measuring specific antibody levels to the 35 human adenovirus serotypes in Vero cells. The test is read at 5 days by macroscopic observation after staining the uninfected cells with crystal violet.
P G Bingham, J C Hierholzer
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Immunologic Relationships Among the Spotted Fever Group of Rickettsias Determined by Toxin Neutralization Tests in Mice with Convalescent Animal Serums [PDF]
Journal of Immunology, 1960 Tests of certain variables inherent in the spotted fever toxin neutralization test in mice revealed that a) spotted fever group toxins remained stable for a period of at least 3 hr at either room temperature (26°C) or at 2°C, b) serum antitoxin titers ...
E. John Bell, Herbert G. Stoenner
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Variability in dengue titer estimates from plaque reduction neutralization tests poses a challenge to epidemiological studies and vaccine development. [PDF]
PLoS Negl Trop Dis, 2014 Background Accurate determination of neutralization antibody titers supports epidemiological studies of dengue virus transmission and vaccine trials. Neutralization titers measured using the plaque reduction neutralization test (PRNT) are believed to ...
Salje H +9 more
europepmc +2 more sources
Frontiers in Microbiology, 2023 Feline calicivirus (FCV) causes upper respiratory tract diseases and even death in cats, thereby acting as a great threat to feline animals. Currently, FCV prevention is mainly achieved through vaccination, but the effectiveness of vaccination is limited.
Yupeng Yang +11 more
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A single mutation in the envelope protein modulates flavivirus antigenicity, stability, and pathogenesis [PDF]
, 2017 The structural flexibility or 'breathing' of the envelope (E) protein of flaviviruses allows virions to sample an ensemble of conformations at equilibrium.
Diamond, Michael S +4 more
core +11 more sources