Results 1 to 10 of about 985,959 (218)

Next generation sequencing

Indian Journal of Medical and Paediatric Oncology, 2020
Introduction microRNAs (miRNAs) have critical roles in tumorigenesis and cancer metastasis. Despite the importance of these small molecules, considerable number of miRNAs have not been identified or experimentally validated yet.
Guruprasad Bhat, Amit Kumar Jain
doaj   +2 more sources

Coming of age: ten years of next-generation sequencing technologies

Nature Reviews Genetics, 2016
John D Mcpherson, W Richard Mccombie
exaly   +2 more sources

Demystifying the Discussion of Sequencing Panel Size in Oncology Genetic Testing

European Medical Journal, 2022
Clinical laboratories worldwide are implementing next-generation sequencing (NGS) to identify cancer genomic variants and ultimately improve patient outcomes. The ability to massively sequence the entire genome or exome of tumour cells has been critical
Cecília Durães, Carla Pereira Gomes, Jose Luis Costa, Luca Quagliata   +3 more
doaj   +1 more source

Metagenomic Analysis of Respiratory RNA Virome of Children with and without Severe Acute Respiratory Infection from the Free State, South Africa during COVID-19 Pandemic Reveals Higher Diversity and Abundance in Summer Compared with Winter Period

Viruses, 2022
Viral respiratory infections contribute to significant morbidity and mortality in children. Currently, there are limited reports on the composition and abundance of the normal commensal respiratory virome in comparison to those in severe acute ...
Ayodeji E. Ogunbayo, Milton T. Mogotsi, Hlengiwe Sondlane, Kelebogile R. Nkwadipo, Saheed Sabiu, Martin M. Nyaga   +5 more
doaj   +1 more source

Next-Generation Sequencing Technology: Current Trends and Advancements

Biology, 2023
Simple Summary Next-generation sequencing (NGS) is a powerful tool used in genomics research. NGS can sequence millions of DNA fragments at once, providing detailed information about the structure of genomes, genetic variations, gene activity, and ...
Heena Satam, Kandarp Joshi, Upasana Mangrolia, Sanober Waghoo, Gulnaz Zaidi, Shravani Rawool, Ritesh P Thakare, Shahid Banday, A. Mishra, Gautam Das, S. K. Malonia   +10 more
semanticscholar   +1 more source

ResFinder – an open online resource for identification of antimicrobial resistance genes in next-generation sequencing data and prediction of phenotypes from genotypes

Microbial Genomics, 2022
Antimicrobial resistance (AMR) is one of the most important health threats globally. The ability to accurately identify resistant bacterial isolates and the individual antimicrobial resistance genes (ARGs) is essential for understanding the evolution and
A. F. Florensa, R. Kaas, P. Clausen, D. Aytan-Aktug, F. Aarestrup   +4 more
semanticscholar   +1 more source

In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4T

Scientific Reports, 2022
Gulosibacter molinativorax ON4T is the only known organism to produce molinate hydrolase (MolA), which catalyses the breakdown of the thiocarbamate herbicide into azepane-1-carboxylic acid (ACA) and ethanethiol.
A. R. Lopes, E. Bunin, A. T. Viana, H. Froufe, A. Muñoz-Merida, D. Pinho, J. Figueiredo, C. Barroso, I. Vaz-Moreira, X. Bellanger, C. Egas, O. C. Nunes   +11 more
doaj   +1 more source

Methods to improve the accuracy of next-generation sequencing

Frontiers in Bioengineering and Biotechnology, 2023
Next-generation sequencing (NGS) is present in all fields of life science, which has greatly promoted the development of basic research while being gradually applied in clinical diagnosis.
Chu Cheng, Zhongjie Fei, Pengfeng Xiao
semanticscholar   +1 more source

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
S. Hassan, R. Bahar, M. F. Johan, Ezzeddin Kamil Mohamed Hashim, W. Abdullah, E. Esa, Faidatul Syazlin Abdul Hamid, Z. Zulkafli   +7 more
semanticscholar   +1 more source

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

BioTechniques, 2019
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat, Nicola Pitsch, Caroline Fromont, Caroline Janitz   +3 more
doaj   +1 more source