Results 91 to 100 of about 1,444,385 (351)
Clinical Infectious Diseases, 2018 Background Metagenomic next-generation sequencing (mNGS) was suggested to potentially replace traditional microbiological methodology because of its comprehensiveness.
Qing Miao +17 more
semanticscholar +1 more source
The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
Frontiers in Cellular and Infection Microbiology, 2021 Lower respiratory tract infections are associated with high morbidity and mortality and significant clinical harm. Due to the limited ability of traditional pathogen detection methods, anti-infective therapy is mostly empirical.
Yan Zheng, X. Qiu, Ting Wang, Jie Zhang
semanticscholar +1 more source
Diagnostic applications of next generation sequencing: working towards quality standards [PDF]
, 2012 Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Adey +42 more
core +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Advancements in breeding Fusarium resistant vanilla varieties using Next Generation Sequencing [PDF]
, 2019 Demand for natural vanilla flavor is increasing, but the cultivated Vanilla planifolia faces critical challenges in response to biotic and abiotic stresses. In particular, Root and Stem Rot (RSR) disease caused by Fusarium oxysporum f. sp.
Besse, Pascale +4 more
core
FEBS Letters, EarlyView.Phototrophs evolved light‐harvesting systems adapted for efficient photon capture in habitats enriched in far‐red radiation. A subset of eukaryotic pigment‐binding proteins can absorb far‐red photons via low‐energy chlorophyll states known as red forms.
Antonello Amelii +8 more
wiley +1 more source
MISSEL: a method to identify a large number of small species-specific genomic subsequences and its application to viruses classification [PDF]
, 2016 Continuous improvements in next generation sequencing technologies led to ever-increasing collections of genomic sequences, which have not been easily characterized by biologists, and whose analysis requires huge computational effort.
Babakir Mina, Muhammed +10 more
core +2 more sources
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
FEBS Letters, EarlyView.The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
ParMap, an Algorithm for the Identification of Complex Genomic Variations in Nextgen Sequencing Data [PDF]
, 2010 Next-generation sequencing produces high-throughput data, albeit with greater error and shorter reads than traditional Sanger sequencing methods. This complicates the detection of genomic variations, especially, small insertions and deletions.
Adolfo A. Ferrando +4 more
core +1 more source
Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1
FEBS Letters, EarlyView.Tgl1, one of yeast sterol ester hydrolases, had been found on the lipid droplets where sterol esters are mainly stored. This study revealed that Tgl1 is transported into the vacuole depending on the ESCRT‐I–III complex, and that it exhibits intra‐vacuolar sterol ester hydrolase activity.
Takumi Nakatsuji +5 more
wiley +1 more source