Results 131 to 140 of about 5,736,241 (346)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Symposium NGS 2016 - ‘Next generation sequencing’ technology in routine analysis in Belgian healthcare system (EN)

, 2016
‘Next generation sequencing’ technology in routine analysis in Belgian healthcare system.
M. Van den Bulcke
core  

Maybe More Than Mates, Maybe Not? The Modern Meaning of a Modern Alliance

, 2022
Article in Next-generation perspectives on the US–Australia alliance (Next Generation Perspectives Policy Paper; August 2022)Benjamin Cherry-Smith, Renee Cremer, Monique Currie and Jena ...
Cremer, R., Cherry-Smith, B., Jaensch, J., Currie, M.   +3 more
core  

Overview of Next Generation Sequencing Technologies

Current Protocols in Molecular Biology, 2018
High throughput DNA sequencing methodology (next generation sequencing; NGS) has rapidly evolved over the past 15 years and new methods are continually being commercialized.
B. Slatko, Andy Gardner, F. M. Ausubel
semanticscholar   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

The Progress of Next Generation Sequencing in the Assessment of Myeloid Malignancies

, 2019
The introduction and advances on next-generation sequencing have led to novel ways to integrate simultaneous assessment of multiple target genes in routine laboratory analysis.
Fulya Öz Puyan, Serhan Alkan, Alkan, Serhan, Puyan, Fulya Öz   +3 more
core   +1 more source

Deciphering the Natural Reassortment Dynamics of Infectious Bursal Disease Virus, Isolated from Field Outbreaks in Southern India, Through Complete Genome Sequencing

Pathogens
The present study was carried out to analyze the complete genome sequences of infectious bursal disease virus (IBDV) isolates obtained from field outbreaks in the southern regions of India.
Raja Paramasivam, Megan Justice, Tuticorin Maragatham Alagesan Senthilkumar, Manoharan Parthiban, Ardhanary Thangavelu, Angappan Mangala Gowri, Ramasamy Bharathi, Hong Hwang, Jerry Malayer, Samuel Pushparaj   +9 more
doaj   +1 more source

Graphical Workflow System for Modification Calling by Machine Learning of Reverse Transcription Signatures

Frontiers in Genetics, 2019
Modification mapping from cDNA data has become a tremendously important approach in epitranscriptomics. So-called reverse transcription signatures in cDNA contain information on the position and nature of their causative RNA modifications. Data mining of,
Lukas Schmidt, Stephan Werner, Thomas Kemmer, Stefan Niebler, Marco Kristen, Lilia Ayadi, Lilia Ayadi, Patrick Johe, Virginie Marchand, Tanja Schirmeister, Yuri Motorin, Yuri Motorin, Andreas Hildebrandt, Bertil Schmidt, Mark Helm   +14 more
doaj   +1 more source

Comparison of Next-Generation Sequencing Systems

Journal of Biomedicine and Biotechnology, 2012
With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life ...
Lin Liu, Yin-Hu Li, Siliang Li, Ni Hu, Yi-Min He, Raymond S. Pong, Dan-Ni Lin, Lihua Lu, Maggie Law   +8 more
semanticscholar   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source