Results 11 to 20 of about 942,883 (343)
Next-generation sequencing: applications beyond genomes [PDF]
, 2008 The development of DNA sequencing more than 30 years ago has profoundly impacted biological research. In the last couple of years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of complex samples at
Samuel Marguerat +2 more
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A normalization technique for next generation sequencing experiments [PDF]
, 2010 Next generation sequencing (NGS) are these days one of the key technologies in biology. NGS' cost effectiveness and capability of finding the smallest variations in the genome makes them increasingly popular.
Günter Klambauer +3 more
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Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +11 more sources
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
core +1 more source
Next-Generation Sequencing Technologies [PDF]
Cold Spring Harbor Perspectives in Medicine, 2018 Although DNA and RNA sequencing has a history spanning five decades, large-scale massively parallel sequencing, or next-generation sequencing (NGS), has only been commercially available for about 10 years. Nonetheless, the meteoric increase in sequencing throughput with NGS has dramatically changed our understanding of our genome and ourselves ...
McCombie, W Richard +2 more
openaire +4 more sources
Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) [PDF]
PeerJ, 2018 Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular ...
Weiping Shi +12 more
doaj +2 more sources
High-throughput Quant-iT PicoGreen assay using an automated liquid handling system
BioTechniques, 2019 Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat +3 more
doaj +1 more source
Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity
Cell Reports, 2023 Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi +19 more
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Next-generation sequencing [PDF]
Breast Cancer Research, 2009 Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to characterise cancers at the genomic, transcriptomic and epigenetic levels. Cataloguing all mutations, copy number aberrations and somatic rearrangements in an entire cancer genome at base pair resolution can now be performed in a ...
openaire +3 more sources
Pathogens, 2020 Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi +8 more
doaj +1 more source