Results 11 to 20 of about 964,159 (277)

Next generation sequencing technologies for next generation plant breeding [PDF]

Frontiers in Plant Science, 2014
SOHAM eRAY, PRATIK eSATYA
doaj   +2 more sources

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot, A. E. Pozhitkov, A. Halperin, A. Harrison, A. Ott, Amend, B. M. Pettitt, Berger, Binder, Binder, Binder, Binder, Bolstad, Bullard, Burden, Burden, C. Gibas, C. J. Burden, Chou, Chou, Czypionka, D. P. Kreil, D. Tautz, E. Carlon, Fasold, Fasold, Fiche, Fuchs, H. Binder, Halperin, Harr, Harrison, He, Heim, Held, Hooyberghs, Huettel, Iltumur, Irizarry, Irizarry, Irizarry, Irving, J. Hooyberghs, Kane, L. J. Gamble, Lee, Lee, Letowski, Li, Liebich, Lockhart, Luebke, Marshall, Matveeva, Mueckstein, Mulders, Naiser, Naiser, P. A. Noble, Pingel, Pozhitkov, R. Levicky, Relogio, Rouillard, Tanaka, Trapp, Upton, Vainrub, Wodicka, Yu, Zhang   +70 more
core   +11 more sources

Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]

, 2020
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer, Garber, Megan E, Hillson, Nathan J, Mukhopadhyay, Aindrila   +3 more
core   +1 more source

In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4T

Scientific Reports, 2022
Gulosibacter molinativorax ON4T is the only known organism to produce molinate hydrolase (MolA), which catalyses the breakdown of the thiocarbamate herbicide into azepane-1-carboxylic acid (ACA) and ethanethiol.
A. R. Lopes, E. Bunin, A. T. Viana, H. Froufe, A. Muñoz-Merida, D. Pinho, J. Figueiredo, C. Barroso, I. Vaz-Moreira, X. Bellanger, C. Egas, O. C. Nunes   +11 more
doaj   +1 more source

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) [PDF]

PeerJ, 2018
Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular ...
Weiping Shi, Jingye Cheng, Xiaojie Wen, Jixiang Wang, Guanyan Shi, Jiayan Yao, Liyuan Hou, Qian Sun, Peng Xiang, Xiangyang Yuan, Shuqi Dong, Pingyi Guo, Jie Guo   +12 more
doaj   +2 more sources

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

BioTechniques, 2019
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat, Nicola Pitsch, Caroline Fromont, Caroline Janitz   +3 more
doaj   +1 more source

Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity

Cell Reports, 2023
Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi, Christopher Wedeles, Daqi Xu, Krzysztof Kolmus, Sarah E. Headland, Grace Teng, Joseph Guillory, Yi Jimmy Zeng, Tommy K. Cheung, Subhra Chaudhuri, Zora Modrusan, Yuxin Liang, Stuart Horswell, Benjamin Haley, Sascha Rutz, Christopher Rose, Yvonne Franke, Donald S. Kirkpatrick, Jason A. Hackney, Mark S. Wilson   +19 more
doaj   +1 more source

Uncovering the First Atypical DS-1-like G1P[8] Rotavirus Strains That Circulated during Pre-Rotavirus Vaccine Introduction Era in South Africa

Pathogens, 2020
Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi, Milton T. Mogotsi, Sebotsana P. Rasebotsa, Mapaseka L. Seheri, M. Jeffrey Mphahlele, Valantine N. Ndze, Francis E. Dennis, Khuzwayo C. Jere, Martin M. Nyaga   +8 more
doaj   +1 more source

Genomic Analysis of G2P[4] Group A Rotaviruses in Zambia Reveals Positive Selection in Amino Acid Site 7 of Viral Protein 3

Viruses, 2023
The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi, Robyn-Lee Potgieter, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Milton T. Mogotsi, Nonkululeko Magagula, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +10 more
doaj   +1 more source

BamView: visualizing and interpretation of next-generation sequencing read alignments. [PDF]

, 2012
So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes.
Berriman, Matthew, Carver, Tim, Harris, Simon R., McQuillan, Jacqueline A., Otto, Thomas D., Parkhill, Julian   +5 more
core   +1 more source