Results 21 to 30 of about 1,368,122 (234)

A genome-wide survey of mutations in the Jurkat cell line

BMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su   +4 more
doaj   +1 more source

Whole-Genome Analyses Identifies Multiple Reassortant Rotavirus Strains in Rwanda Post-Vaccine Introduction

Viruses, 2021
Children in low-and middle-income countries, including Rwanda, experience a greater burden of rotavirus disease relative to developed countries. Evolutionary mechanisms leading to multiple reassortant rotavirus strains have been documented over time ...
Sebotsana Rasebotsa, Jeannine Uwimana, Milton T. Mogotsi, Kebareng Rakau, Nonkululeko B. Magagula, Mapaseka L. Seheri, Jason M. Mwenda, M. Jeffrey Mphahlele, Saheed Sabiu, Richard Mihigo, Leon Mutesa, Martin M. Nyaga   +11 more
doaj   +1 more source

Next Generation Sequencing Analysis Reveals Segmental Patterns of microRNA Expression in Mouse Epididymal Epithelial Cells

PLoS ONE, 2015
The functional maturation of mammalian spermatozoa is accomplished as the cells descend through the highly specialized microenvironment of the epididymis.
Brett Nixon, Simone J. Stanger, Bettina P Mihalas, Jackson N. Reilly, A. Anderson, Matthew D. Dun, Sonika Tyagi, J. Holt, Eileen A. McLaughlin   +8 more
semanticscholar   +1 more source

Next-generation sequencing: insights to advance clinical investigations of the microbiome

Journal of Clinical Investigation, 2022
Next-generation sequencing (NGS) technology has advanced our understanding of the human microbiome by allowing for the discovery and characterization of unculturable microbes with prediction of their function.
Caroline R. Wensel, J. Pluznick, S. Salzberg, C. Sears   +3 more
semanticscholar   +1 more source

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

Nucleic Acids Research, 2012
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth, E. Pruesse, Timmy Schweer, J. Peplies, Christian Quast, M. Horn, F. Glöckner   +6 more
semanticscholar   +1 more source

Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center

Microbiome, 2019
Background Viruses play an important role in ecosystems, including the built environment (BE). While numerous studies have characterized bacterial and fungal microbiomes in the BE, few have focused on the viral microbiome (virome).
Aaron J. Prussin, Pedro J. Torres, John Shimashita, Steven R. Head, Kyle J. Bibby, Scott T. Kelley, Linsey C. Marr   +6 more
doaj   +1 more source

Genomic Analysis of Rwandan G9P[8] Rotavirus Strains Pre- and Post-RotaTeq^® Vaccine Reveals Significant Distinct Sub-Clustering in a Post-Vaccination Cohort

Viruses, 2023
Although the introduction of rotavirus vaccines has substantially contributed to the reduction in rotavirus morbidity and mortality, concerns persist about the re-emergence of variant strains that might alter vaccine effectiveness in the long term.
Robyn-Lee Potgieter, Peter N. Mwangi, Milton T. Mogotsi, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Mapaseka L. Seheri, A. Duncan Steele, Jason M. Mwenda, Martin M. Nyaga   +11 more
doaj   +1 more source

BamView: visualizing and interpretation of next-generation sequencing read alignments. [PDF]

, 2012
So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes.
Berriman, Matthew, Carver, Tim, Harris, Simon R., McQuillan, Jacqueline A., Otto, Thomas D., Parkhill, Julian   +5 more
core   +1 more source

The Evolution of Post-Vaccine G8P[4] Group a Rotavirus Strains in Rwanda; Notable Variance at the Neutralization Epitope Sites

Pathogens, 2023
Africa has a high level of genetic diversity of rotavirus strains, which is suggested to be a possible reason contributing to the suboptimal effectiveness of rotavirus vaccines in this region.
Peter N. Mwangi, Robyn-Lee Potgieter, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Jason M. Mwenda, Milton T. Mogotsi, Kebareng Rakau, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +13 more
doaj   +1 more source

ANGSD: Analysis of Next Generation Sequencing Data

BMC Bioinformatics, 2014
High-throughput DNA sequencing technologies are generating vast amounts of data. Fast, flexible and memory efficient implementations are needed in order to facilitate analyses of thousands of samples simultaneously.
T. Korneliussen, A. Albrechtsen, R. Nielsen   +2 more
semanticscholar   +1 more source