Results 21 to 30 of about 964,159 (277)

A genome-wide survey of mutations in the Jurkat cell line

BMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su   +4 more
doaj   +1 more source

Metagenomic Analysis of the Enteric RNA Virome of Infants from the Oukasie Clinic, North West Province, South Africa, Reveals Diverse Eukaryotic Viruses

Viruses, 2020
Establishing a diverse gut microbiota after birth is essential for preventing illnesses later in life. However, little knowledge exists about the total viral population (virome) present in the gut of infants during the early developmental stage, with RNA
Milton T. Mogotsi, Peter N. Mwangi, Phillip A. Bester, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Hester G. O’Neill, Martin M. Nyaga   +6 more
doaj   +1 more source

Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells

Frontiers in Immunology, 2022
The transcriptomic profile of a cell population can now be studied at the cellular level using single-cell mRNA sequencing (scRNA-seq). This novel technique provides the unprecedented opportunity to explore the cellular composition of the bronchoalveolar
Sophie E. Sage, Pamela Nicholson, Laureen M. Peters, Tosso Leeb, Tosso Leeb, Vidhya Jagannathan, Vinzenz Gerber   +6 more
doaj   +1 more source

A normalization technique for next generation sequencing experiments [PDF]

, 2010
Next generation sequencing (NGS) are these days one of the key technologies in biology. NGS' cost effectiveness and capability of finding the smallest variations in the genome makes them increasingly popular.
Andreas Mayr, Günter Klambauer, Günter Klambauer, Karin Schwarzbauer, Sepp Hochreiter   +4 more
core   +3 more sources

Whole Genome Analysis of Human Rotaviruses Reveals Single Gene Reassortant Rotavirus Strains in Zambia

Viruses, 2021
Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis.
Wairimu M. Maringa, Julia Simwaka, Peter N. Mwangi, Evans M. Mpabalwani, Jason M. Mwenda, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga   +7 more
doaj   +1 more source

Whole-Genome Analyses Identifies Multiple Reassortant Rotavirus Strains in Rwanda Post-Vaccine Introduction

Viruses, 2021
Children in low-and middle-income countries, including Rwanda, experience a greater burden of rotavirus disease relative to developed countries. Evolutionary mechanisms leading to multiple reassortant rotavirus strains have been documented over time ...
Sebotsana Rasebotsa, Jeannine Uwimana, Milton T. Mogotsi, Kebareng Rakau, Nonkululeko B. Magagula, Mapaseka L. Seheri, Jason M. Mwenda, M. Jeffrey Mphahlele, Saheed Sabiu, Richard Mihigo, Leon Mutesa, Martin M. Nyaga   +11 more
doaj   +1 more source

Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center

Microbiome, 2019
Background Viruses play an important role in ecosystems, including the built environment (BE). While numerous studies have characterized bacterial and fungal microbiomes in the BE, few have focused on the viral microbiome (virome).
Aaron J. Prussin, Pedro J. Torres, John Shimashita, Steven R. Head, Kyle J. Bibby, Scott T. Kelley, Linsey C. Marr   +6 more
doaj   +1 more source

A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing. [PDF]

, 2012
Background. Next-generation sequencing of cancers has identified important therapeutic targets and biomarkers. The goal of this pilot study was to compare the genetic changes in a human papillomavirus- (HPV-)positive and an HPV-negative head and neck ...
Barrett, John W, Basmaji, John, Boutros, Paul, Chan-Seng-Yue, Michelle, Dhaliwal, Sandeep, Dowthwaite, Samuel, Franklin, Jason H, Fung, Kevin, Koropatnick, James, Kwan, Keith, Lambin, Philippe, Mymryk, Joe S, Nichols, Anthony C, Palma, David A, Starmans, Maud HW, Szeto, Christopher CT, Todorovic, Biljana, Wehrli, Bret, Xu, Wei, Yoo, John   +19 more
core   +3 more sources

Genomic Analysis of Rwandan G9P[8] Rotavirus Strains Pre- and Post-RotaTeq^® Vaccine Reveals Significant Distinct Sub-Clustering in a Post-Vaccination Cohort

Viruses, 2023
Although the introduction of rotavirus vaccines has substantially contributed to the reduction in rotavirus morbidity and mortality, concerns persist about the re-emergence of variant strains that might alter vaccine effectiveness in the long term.
Robyn-Lee Potgieter, Peter N. Mwangi, Milton T. Mogotsi, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Mapaseka L. Seheri, A. Duncan Steele, Jason M. Mwenda, Martin M. Nyaga   +11 more
doaj   +1 more source

Pyrosequencing/Sanger Plant Genome Assembly (Limitations, Problems And Solutions) - On The Way To Cucumber (Cucumis sativus L. cv. Borszczagowski) Draft Genome Sequence Publishing [PDF]

, 2010
Genome sequencing give us an opportunity to broaden the range and improve the quality of omics studies. New sequencing technologies make it possible to achieve the sequencing reads fast and cheap.
Rafal Woycicki, Zbigniew Przybecki
core   +2 more sources