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Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]

open access: yes, 2020
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer   +3 more
core   +1 more source

A genome-wide survey of mutations in the Jurkat cell line

open access: yesBMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia   +4 more
doaj   +1 more source

Whole-Genome Analyses Identifies Multiple Reassortant Rotavirus Strains in Rwanda Post-Vaccine Introduction

open access: yesViruses, 2021
Children in low-and middle-income countries, including Rwanda, experience a greater burden of rotavirus disease relative to developed countries. Evolutionary mechanisms leading to multiple reassortant rotavirus strains have been documented over time ...
Sebotsana Rasebotsa   +11 more
doaj   +1 more source

Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center

open access: yesMicrobiome, 2019
Background Viruses play an important role in ecosystems, including the built environment (BE). While numerous studies have characterized bacterial and fungal microbiomes in the BE, few have focused on the viral microbiome (virome).
Aaron J. Prussin   +6 more
doaj   +1 more source

Genomic Analysis of Rwandan G9P[8] Rotavirus Strains Pre- and Post-RotaTeq® Vaccine Reveals Significant Distinct Sub-Clustering in a Post-Vaccination Cohort

open access: yesViruses, 2023
Although the introduction of rotavirus vaccines has substantially contributed to the reduction in rotavirus morbidity and mortality, concerns persist about the re-emergence of variant strains that might alter vaccine effectiveness in the long term.
Robyn-Lee Potgieter   +11 more
doaj   +1 more source

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

open access: yes, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot   +70 more
core   +3 more sources

The Evolution of Post-Vaccine G8P[4] Group a Rotavirus Strains in Rwanda; Notable Variance at the Neutralization Epitope Sites

open access: yesPathogens, 2023
Africa has a high level of genetic diversity of rotavirus strains, which is suggested to be a possible reason contributing to the suboptimal effectiveness of rotavirus vaccines in this region.
Peter N. Mwangi   +13 more
doaj   +1 more source

BamView: visualizing and interpretation of next-generation sequencing read alignments. [PDF]

open access: yes, 2012
So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes.
Berriman, Matthew   +5 more
core   +1 more source

Neuroinflammation regulates the balance between hippocampal neuron death and neurogenesis in an ex vivo model of thiamine deficiency

open access: yesJournal of Neuroinflammation, 2022
Background Thiamine (vitamin B1) is a cofactor for enzymes of central energy metabolism and its deficiency (TD) impairs oxidative phosphorylation, increases oxidative stress, and activates inflammatory processes that can lead to neurodegeneration ...
Larissa M. G. Cassiano   +4 more
doaj   +1 more source

Next-Generation Sequencing: The Race Is On [PDF]

open access: yesCell, 2008
The $1000 genome may still be years away, but with the arrival of next-generation sequencing (NGS) technologies that are much faster and cheaper than the traditional Sanger method, large-scale sequencing of hundreds or even thousands of human genomes is fast becoming reality.
openaire   +2 more sources

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