Results 51 to 60 of about 1,444,385 (351)

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases

Genes, 2022
Next-generation sequencing (NGS) technologies have become increasingly available for use in the clinical microbiology diagnostic environment. There are three main applications of these technologies in the clinical microbiology laboratory: whole genome ...
E. Hilt, P. Ferrieri
semanticscholar   +1 more source

Decreased exosome-delivered miR-486-5p is responsible for the peritoneal metastasis of gastric cancer cells by promoting EMT progress

World Journal of Surgical Oncology, 2021
Background The present study aims to investigate the preliminary mechanism underlying the peritoneal metastasis of gastric cancer cells. Methods Exosomes from GC9811 cells (Con-Exo) and from GC9811-P cells (PM-Exo) were extracted by ultracentrifugation ...
Xian-Ming Lin, Zhi-Jiang Wang, Yu-Xiao Lin, Hao Chen   +3 more
doaj   +1 more source

Application of Next Generation Sequencing in Laboratory Medicine

Annals of Laboratory Medicine, 2021
The rapid development of next-generation sequencing (NGS) technology, including advances in sequencing chemistry, sequencing technologies, bioinformatics, and data interpretation, has facilitated its wide clinical application in precision medicine.
Y. Zhong, Feng Xu, Jinhua Wu, Jeffrey Schubert, Marilyn M. Li   +4 more
semanticscholar   +1 more source

Base calling for high-throughput short-read sequencing: dynamic programming solutions [PDF]

, 2013
Shreepriya Das and Haris Vikalo are with the Electrical and Computer Engineering Department, The University of Texas at Austin, Austin, Texas 78712, USABackground: Next-generation DNA sequencing platforms are capable of generating millions of reads in ...
Das, Shreepriya, Vikalo, Haris
core   +3 more sources

Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells

eLife, 2021
Tissue homeostasis requires long-term lineage fidelity of somatic stem cells. Whether and how age-related changes in somatic stem cells impact the faithful execution of lineage decisions remains largely unknown.
Helen M Tauc, Imilce A Rodriguez-Fernandez, Jason A Hackney, Michal Pawlak, Tal Ronnen Oron, Jerome Korzelius, Hagar F Moussa, Subhra Chaudhuri, Zora Modrusan, Bruce A Edgar, Heinrich Jasper   +10 more
doaj   +1 more source

Next generation sequencing [PDF]

, 2013
Informacije pohranjene u DNA sekvenci sadrži vrijedne podatke o tom organizmu, i znajući sekvencu cijelog genoma, u mogućnosti smo razumjeti procese koji se odvijaju u tom biću.
Vučenović, Dunja
core   +2 more sources

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

High Detection Frequency of Vaccine-Associated Polioviruses and Non-Polio Enteroviruses in the Stools of Asymptomatic Infants from the Free State Province, South Africa

Microorganisms
Enterovirus (EV) infections are widespread and associated with a range of clinical conditions, from encephalitis to meningitis, gastroenteritis, and acute flaccid paralysis.
Milton T. Mogotsi, Ayodeji E. Ogunbayo, Hester G. O’Neill, Martin M. Nyaga   +3 more
doaj   +1 more source

Molecular Characterisation of a Rare Reassortant Porcine-Like G5P[6] Rotavirus Strain Detected in an Unvaccinated Child in Kasama, Zambia

Pathogens, 2020
A human-porcine reassortant strain, RVA/Human-wt/ZMB/UFS-NGS-MRC-DPRU4723/2014/G5P[6], was identified in a sample collected in 2014 from an unvaccinated 12 month old male hospitalised for gastroenteritis in Zambia.
Wairimu M. Maringa, Peter N. Mwangi, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Ina Peenze, Mathew D. Esona, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga   +9 more
doaj   +1 more source