Results 1 to 10 of about 8,732 (191)

NifH- Harboring Bacterial Community Composition Across an Alaskan Permafrost Thaw Gradient [PDF]

open access: yesFrontiers in Microbiology, 2016
Since nitrogen (N) is often limiting in permafrost soils, we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal
Christopher Ryan Penton   +16 more
doaj   +6 more sources

Undervalued Pseudo-nifH Sequences in Public Databases Distort Metagenomic Insights into Biological Nitrogen Fixers

open access: yesmSphere, 2021
Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise   +3 more
doaj   +2 more sources

Seasonal and long-term effects of nutrient additions and liming on the nifH gene in cerrado soils under native vegetation

open access: yesiScience, 2021
Summary: Biological nitrogen fixation (BNF) represents the main input source of N in tropical savannas. BNF could be particularly important for Brazilian savannas (known as Cerrado) that show a highly conservative N cycle.
Rafaella Silveira   +7 more
doaj   +2 more sources

Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]

open access: yesmBio
NifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie   +6 more
doaj   +2 more sources

Evaluation of Primers Targeting the Diazotroph Functional Gene and Development of NifMAP – A Bioinformatics Pipeline for Analyzing nifH Amplicon Data

open access: yesFrontiers in Microbiology, 2018
Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four ...
Roey Angel   +6 more
doaj   +2 more sources

Widespread Distribution and Expression of Gamma A (UMB), an Uncultured, Diazotrophic, γ-Proteobacterial nifH Phylotype. [PDF]

open access: yesPLoS ONE, 2015
Marine dinitrogen (N2) fixation studies have focused nearly exclusively on cyanobacterial diazotrophs; however γ-proteobacteria are an abundant component of the marine community and have been largely overlooked until recently.
Rebecca Langlois   +4 more
doaj   +2 more sources

NIFtHool: an informatics program for identification of NifH proteins using deep neural networks [version 1; peer review: 2 approved]

open access: yesF1000Research, 2022
Atmospheric nitrogen fixation carried out by microorganisms has environmental and industrial importance, related to the increase of soil fertility and productivity.
Marco Esteban Gudiño Gomezjurado   +5 more
doaj   +2 more sources

A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]

open access: yesPLoS ONE, 2012
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
doaj   +2 more sources

Nitrogen Fixation Aligns with nifH Abundance and Expression in Two Coral Trophic Functional Groups

open access: yesFrontiers in Microbiology, 2017
Microbial nitrogen fixation (diazotrophy) is a functional trait widely associated with tropical reef-building (scleractinian) corals. While the integral role of nitrogen fixation in coral nutrient dynamics is recognized, its ecological significance ...
Claudia Pogoreutz   +9 more
doaj   +2 more sources

Analysis of nifH DNA and RNA reveals a disproportionate contribution to nitrogenase activities by rare plankton-associated diazotrophs

open access: yesBMC Microbiology, 2019
Background Holobionts comprising nitrogen-fixing diazotrophs and phytoplankton or zooplankton are ubiquitous in the pelagic sea. However, neither the community structure of plankton-associated diazotrophs (PADs) nor their nitrogenase transcriptional ...
Qing-Song Yang   +8 more
doaj   +2 more sources

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