Results 141 to 150 of about 5,029 (176)

Nucleotide sequence of the gene encoding the nitrogenase iron protein (nifH) of Azospirillum brasilense and identification of a region controlling nifH transcription

Molecular Genetics and Genomics, 1989
The DNA sequence was determined for the Azospirillum brasilense nifH gene and part of the nifD gene. The nifH gene is 885 bp long and encodes 293 amino acid residues. The region upstream of the nifH open reading frame contains a putative promoter whose sequence shows perfect homology with promoters of other diazotrophic bacteria and two putative ...
Marco Bazzicalupo, Renato Fani, Mario Polsinelli   +2 more
exaly   +4 more sources

Frameshifts close to the Klebsiella pneumoniae nifH promoter prevent multicopy inhibition by hybrid nifH plasmids

Molecular and General Genetics MGG, 1987
Certain multicopy plasmids bearing promoter sequences of nitrogen fixation (nif) genes inhibit expression of chromosomal genes in Nif+ Klebsiella pneumoniae, hence leading to a Nif- phenotype. This 'multicopy inhibition' has been attributed to the titration of the nif-specific activator protein NifA by the plasmid-borne promoter sequences.
M, Buck, W, Cannon
openaire   +2 more sources

nifH homologs from soil metagenome

Ekologija, 2011
Biological nitrogen fixation is an important process that allows nitrogen uptake into biological systems. But it is still unclear from where the first nif gene was formed or evolved. Soil samples from different agro-climatic zones of western Indian Himalaya were analysed for the diversity of nifH in their metagenome.
Ravindra Soni, Reeta Goel
openaire   +1 more source

Composition of nifH in a wastewater treatment system reliant on N2 fixation

Applied Microbiology and Biotechnology, 2008
High levels of nitrogen fixation have been observed in the wastewaters of pulp and paper mills. In this study, we show that nitrogen fixation in a model pulp and paper wastewater treatment system is supported by a high density of nifH sequences that are of low diversity. Quantitative PCR revealed a ratio of nifH to 16S rDNA of 1.14 +/- 0.76 which shows
Gareth Lloyd-Jones
exaly   +3 more sources

Fluorescence in situ hybridization for intracellular localization of nifH mRNA

Systematic and Applied Microbiology, 2009
Few reports on in situ mRNA detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. This study reports a reliable approach for the in situ detection of nifH mRNA using fluorescence hybridization based on a previously described protocol for pmoA. nifH
Martin, Pilhofer, Marko, Pavlekovic, Natuschka M, Lee, Wolfgang, Ludwig, Karl-Heinz, Schleifer   +4 more
openaire   +2 more sources

The cloning and functional characterization of the nifH gene of Rhodospirillum rubrum

Gene, 1990
Dinitrogenase reductase (the nifH product) from Rhodospirillum rubrum is regulated by a post-translational modification system encoded by draTG. As demonstrated in this report, the cloning, sequencing, and functional characterization of the nifH gene provides a basis for further analysis as well as revealing interesting features of gene organization ...
L J, Lehman, W P, Fitzmaurice, G P, Roberts   +2 more
openaire   +2 more sources

NifH-based studies on azotobacterial diversity in cotton soils of India

Archives of Microbiology, 2009
In order to promote the use of Azotobacter inoculants for cotton crop, a complete characterization of soil isolates of Azotobacter, isolated and screened on the basis of physiological properties, from four different cotton-wheat cropping regions of India was carried out, and their genetic diversity determined by RFLP (restriction fragment length ...
Ranjana, Bhatia, Silke, Ruppel, Neeru, Narula   +2 more
openaire   +2 more sources

Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat

Microbial Ecology, 2005
N(2) fixation, diazotrophic community composition, and organisms actively expressing genes for N(2) fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA).
T F, Steppe, H W, Paerl
openaire   +2 more sources

EndophyticnifHgene diversity in African sweet potato

Canadian Journal of Microbiology, 2003
A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli.
Birgit, Reiter, Helmut, Bürgmann, Kornel, Burg, Angela, Sessitsch   +3 more
openaire   +2 more sources

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Journal of Environmental Sciences, 2007
Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination.
Zhen-Cheng, Su, Hui-Wen, Zhang, Xin-Yu, Li, Qin, Zhang, Cheng-Gang, Zhang   +4 more
openaire   +2 more sources