Results 141 to 150 of about 6,727 (173)
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nifH homologs from soil metagenome
Ekologija, 2011Biological nitrogen fixation is an important process that allows nitrogen uptake into biological systems. But it is still unclear from where the first nif gene was formed or evolved. Soil samples from different agro-climatic zones of western Indian Himalaya were analysed for the diversity of nifH in their metagenome.
Ravindra Soni, Reeta Goel
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Composition of nifH in a wastewater treatment system reliant on N2 fixation
Applied Microbiology and Biotechnology, 2008High levels of nitrogen fixation have been observed in the wastewaters of pulp and paper mills. In this study, we show that nitrogen fixation in a model pulp and paper wastewater treatment system is supported by a high density of nifH sequences that are of low diversity. Quantitative PCR revealed a ratio of nifH to 16S rDNA of 1.14 +/- 0.76 which shows
Gareth Lloyd-Jones
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Fluorescence in situ hybridization for intracellular localization of nifH mRNA
Systematic and Applied Microbiology, 2009Few reports on in situ mRNA detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. This study reports a reliable approach for the in situ detection of nifH mRNA using fluorescence hybridization based on a previously described protocol for pmoA. nifH
Martin, Pilhofer +4 more
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The cloning and functional characterization of the nifH gene of Rhodospirillum rubrum
Gene, 1990Dinitrogenase reductase (the nifH product) from Rhodospirillum rubrum is regulated by a post-translational modification system encoded by draTG. As demonstrated in this report, the cloning, sequencing, and functional characterization of the nifH gene provides a basis for further analysis as well as revealing interesting features of gene organization ...
L J, Lehman +2 more
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NifH-based studies on azotobacterial diversity in cotton soils of India
Archives of Microbiology, 2009In order to promote the use of Azotobacter inoculants for cotton crop, a complete characterization of soil isolates of Azotobacter, isolated and screened on the basis of physiological properties, from four different cotton-wheat cropping regions of India was carried out, and their genetic diversity determined by RFLP (restriction fragment length ...
Ranjana, Bhatia +2 more
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Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat
Microbial Ecology, 2005N(2) fixation, diazotrophic community composition, and organisms actively expressing genes for N(2) fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA).
T F, Steppe, H W, Paerl
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EndophyticnifHgene diversity in African sweet potato
Canadian Journal of Microbiology, 2003A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli.
Birgit, Reiter +3 more
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Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil
Journal of Environmental Sciences, 2007Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination.
Zhen-Cheng, Su +4 more
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Gene, 1989
Small restriction fragments of the plasmid-borne Enterobacter agglomerans 333 nif region were cloned into a promoter probe plasmid as transcriptional fusions with the lacZ gene. Identification of NifA-dependent promoters was accomplished by using a compatible plasmid which constitutively expresses the Klebsiella pneumoniae nifA gene. beta-Galactosidase
R, Kreutzer, M, Singh, W, Klingmüller
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Small restriction fragments of the plasmid-borne Enterobacter agglomerans 333 nif region were cloned into a promoter probe plasmid as transcriptional fusions with the lacZ gene. Identification of NifA-dependent promoters was accomplished by using a compatible plasmid which constitutively expresses the Klebsiella pneumoniae nifA gene. beta-Galactosidase
R, Kreutzer, M, Singh, W, Klingmüller
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In Rhizobium Japonicum the Nifh and Nifdk Genes are Separated
1984We have shown previously that a cloned HindIII restriction fragment from Rhizobium japonicum 3I1b 110 contains the nitrogenase structural genes nifD and nifK (Fig. 1). However, we have not obtained evidence for the existence of the gene for nitrogenase reductase (nifH) adjacent to th nifDK cluster.
Martin Fuhrmann +2 more
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