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Quantitative Analysis of nifH Genes and Transcripts from Aquatic Environments

2005
The availability of fixed inorganic nitrogen often plays a fundamental role in regulating primary production in both aquatic and terrestrial ecosystems. Because biological nitrogen fixation is an important source of nitrogen in marine environments, the study of N2-fixing microorganisms is of fundamental importance to our understanding of global ...
Steven M, Short, Jonathan P, Zehr
openaire   +2 more sources

Evaluation of PCR primers for universal nifH gene targeting and for assessment of transcribed nifH pools in roots of Oryza longistaminata with and without low nitrogen input.

FEMS microbiology ecology, 2008
The bias of widely used degenerate nifH-specific primer sets was first tested using denaturing gradient gel electrophoresis (DGGE), and their application for profiling of complex communities assessed for roots of Oryza longistaminata. When primers (P) with mismatches at nondegenerate positions were used on genomic DNA of Azotobacter vinelandii, which ...
Moudjahidou, Demba Diallo   +2 more
openaire   +2 more sources

Distribution of Extensive nifH Gene Diversity Across Physical Soil Microenvironments

Microbial Ecology, 2006
The diversity of nitrogen-fixing bacteria is well described for aquatic environments; however, terrestrial analyses remain mostly biased to rhizobial plant-microbe associations. We maximized the level of resolution for this study through the use of nucleotide sequence information extracted from a series of soil microenvironments, ranging from ...
Izquierdo, JA, Nusslein, K
openaire   +2 more sources

[Study of nucleotide sequences of nifH genes in methanotrophic bacteria].

Mikrobiologiia, 2002
Using a previously developed primer system, nifH gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera Methylococcus, Methylocystis and Methylosinus. Fragments of nifH genes were also detected and sequenced in representatives of the genera Methylomonas and ...
E S, Bulygina   +7 more
openaire   +1 more source

5008194 NifH promoters of Bradyrhizobium

Biotechnology Advances, 1991
openaire   +1 more source

Cloning, Nucleotide Sequences and Differential Expression of the nifH and nifH-Like (frxC) Genes from the Filamentous Nitrogen-Fixing Cyanobacterium Plectonema boryanum

Plant and Cell Physiology, 1991
Yuichi Fujita   +4 more
openaire   +1 more source

A nifH-based Oligonucleotide Microarray for Functional Diagnostics of Nitrogen-fixing Microorganisms

Microbial Ecology, 2006
Thomas Hurek   +2 more
exaly  

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