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Quantitative Analysis of nifH Genes and Transcripts from Aquatic Environments
2005The availability of fixed inorganic nitrogen often plays a fundamental role in regulating primary production in both aquatic and terrestrial ecosystems. Because biological nitrogen fixation is an important source of nitrogen in marine environments, the study of N2-fixing microorganisms is of fundamental importance to our understanding of global ...
Steven M, Short, Jonathan P, Zehr
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FEMS microbiology ecology, 2008
The bias of widely used degenerate nifH-specific primer sets was first tested using denaturing gradient gel electrophoresis (DGGE), and their application for profiling of complex communities assessed for roots of Oryza longistaminata. When primers (P) with mismatches at nondegenerate positions were used on genomic DNA of Azotobacter vinelandii, which ...
Moudjahidou, Demba Diallo +2 more
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The bias of widely used degenerate nifH-specific primer sets was first tested using denaturing gradient gel electrophoresis (DGGE), and their application for profiling of complex communities assessed for roots of Oryza longistaminata. When primers (P) with mismatches at nondegenerate positions were used on genomic DNA of Azotobacter vinelandii, which ...
Moudjahidou, Demba Diallo +2 more
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Distribution of Extensive nifH Gene Diversity Across Physical Soil Microenvironments
Microbial Ecology, 2006The diversity of nitrogen-fixing bacteria is well described for aquatic environments; however, terrestrial analyses remain mostly biased to rhizobial plant-microbe associations. We maximized the level of resolution for this study through the use of nucleotide sequence information extracted from a series of soil microenvironments, ranging from ...
Izquierdo, JA, Nusslein, K
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[Study of nucleotide sequences of nifH genes in methanotrophic bacteria].
Mikrobiologiia, 2002Using a previously developed primer system, nifH gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera Methylococcus, Methylocystis and Methylosinus. Fragments of nifH genes were also detected and sequenced in representatives of the genera Methylomonas and ...
E S, Bulygina +7 more
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A nifH-based Oligonucleotide Microarray for Functional Diagnostics of Nitrogen-fixing Microorganisms
Microbial Ecology, 2006Thomas Hurek +2 more
exaly

