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Quantitative Analysis of nifH Genes and Transcripts from Aquatic Environments
2005The availability of fixed inorganic nitrogen often plays a fundamental role in regulating primary production in both aquatic and terrestrial ecosystems. Because biological nitrogen fixation is an important source of nitrogen in marine environments, the study of N2-fixing microorganisms is of fundamental importance to our understanding of global ...
Steven M, Short, Jonathan P, Zehr
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NifH-based studies on azotobacterial diversity in cotton soils of India
Archives of Microbiology, 2009In order to promote the use of Azotobacter inoculants for cotton crop, a complete characterization of soil isolates of Azotobacter, isolated and screened on the basis of physiological properties, from four different cotton-wheat cropping regions of India was carried out, and their genetic diversity determined by RFLP (restriction fragment length ...
Ranjana, Bhatia +2 more
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Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat
Microbial Ecology, 2005N(2) fixation, diazotrophic community composition, and organisms actively expressing genes for N(2) fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA).
T F, Steppe, H W, Paerl
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ПЦР-ИДЕНТИФИКАЦИЯ ГЕНА АЗОТФИКСАЦИИ NIFH У AZOTOBACTER SP
2019В работе представлены результаты апробирования родоспецифичной пары праймеров NifH-g1 F / NifH-g1 R, позволивший выявить ген азотфиксации у изолятов бактерий рода Azotobacter. Выявлен ампликон длиной 400 п. н., специфичный для бактерий Azotobacter sp. In the work the results of approbation of the NifH-g1 F / NifH-g1 R genus-specific primers pair that ...
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A nifH-based Oligonucleotide Microarray for Functional Diagnostics of Nitrogen-fixing Microorganisms
Microbial Ecology, 2006Nitrogen fixation is an important process in biogeochemical cycles exclusively carried out by prokaryotes, mostly by an evolutionarily conserved nitrogenase protein complex, of which one of the structural genes (nifH) is highly valuable for phylogenetic and diversity analyses.
Lei, Zhang +2 more
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In Rhizobium Japonicum the Nifh and Nifdk Genes are Separated
1984We have shown previously that a cloned HindIII restriction fragment from Rhizobium japonicum 3I1b 110 contains the nitrogenase structural genes nifD and nifK (Fig. 1). However, we have not obtained evidence for the existence of the gene for nitrogenase reductase (nifH) adjacent to th nifDK cluster.
Martin Fuhrmann +2 more
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[Study of nucleotide sequences of nifH genes in methanotrophic bacteria].
Mikrobiologiia, 2002Using a previously developed primer system, nifH gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera Methylococcus, Methylocystis and Methylosinus. Fragments of nifH genes were also detected and sequenced in representatives of the genera Methylomonas and ...
E S, Bulygina +7 more
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