Results 21 to 30 of about 8,732 (191)
Analysis of Nitrogenase Fe Protein Activity in Transplastomic Tobacco
Frontiers in Agronomy, 2021 Integration of prokaryotic nitrogen fixation (nif) genes into the plastid genome for expression of functional nitrogenase components could render plants capable of assimilating atmospheric N2 making their crops less dependent of nitrogen fertilizers. The
Jose A. Aznar-Moreno +5 more
doaj +1 more source
New Phytologist, 2021 Bacterial communities form the basis of biogeochemical processes and determine plant growth and health. Mosses harbour diverse bacterial communities that are involved in nitrogen fixation and carbon cycling.
I. Klarenberg +6 more
semanticscholar +1 more source
Contamination of diversenifHandnifH-like DNA into commercial PCR primers [PDF]
FEMS Microbiology Letters, 2005 False-positive results due to DNA contamination in PCR reagents have become a big problem in the amplification of small amounts of DNA. Recently, it was revealed that PCR reagents were contaminated with the nifH (dinitrogenase reductase) gene. We found that the PCR primers supplied by some manufacturers contained nifH gene and nifH-like DNA.
Masahiro, Goto +3 more
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Journal of Phycology, 2022 Cyanobacterial N2‐fixing microorganisms (diazotrophs) play a critical role in nitrogen and carbon cycling in the oceans; hence, accurate measurements of diazotroph abundance are imperative for understanding ocean biogeochemistry.
M. Gradoville +4 more
semanticscholar +1 more source
Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.).
PLoS ONE, 2023 Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution.
Yimin Shang +5 more
doaj +2 more sources
Rhizobium japonicum nitrogenase Fe protein gene (nifH) [PDF]
Journal of Bacteriology, 1984 A 12.1-kilobase PstI fragment from Rhizobium japonicum, which contains homology to both the Klebsiella pneumoniae and the Rhizobium meliloti nifH genes, was cloned into vector pHE3 . The nifH -homologous region was localized on the restriction enzyme cleavage map by Southern blot hybridization experiments. DNA fragments overlapping the R.
M, Fuhrmann, H, Hennecke
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Characterization of nifH mutations of Klebsiella pneumoniae [PDF]
Journal of Bacteriology, 1988 Nucleotide changes in the nifH gene of Klebsiella pneumoniae were identified by DNA cloning and sequencing of six selected mutant strains. The strains were UN60, C-640-GC----TGC; UN116, C-67-TC----TTC; UN117, G-688-AG----AAG; UN1041, CG-302-C----CAC; UN1678, GC-713-C----GTC; and UN1795, G-439-AG----AAG. Their corresponding amino acid substitutions were
C L, Chang +3 more
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Frontiers in Microbiology, 2023 Nitrogenase in some bacteria and archaea catalyzes conversion of N2 to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation)
Minyang Wang +3 more
doaj +1 more source
Detection and characterization of cyanobacterial nifH genes [PDF]
Applied and Environmental Microbiology, 1994 The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
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nifH genes in the obligate methane oxidizing bacteria [PDF]
FEMS Microbiology Letters, 1988 The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH.
Christopher J. Oakley, J. Colin Murrell
openaire +2 more sources