Results 41 to 50 of about 402,220 (244)

Rapid drug-susceptibility testing of Mycobacterium tuberculosis clinical isolates to first-line antitubercular drugs by nitrate reductase assay: A comparison with proportion method

open access: yesInternational Journal of Mycobacteriology, 2016
Objective/background: Early initiation of therapy in patients with tuberculosis is imperative for its control. Conventional methods of susceptibility testing such as the proportion method (PM) require visual detection and counting of colonies that takes ...
Amrish Kohli   +5 more
doaj   +1 more source

Structure and function of nitrate and nitrite transporters, NrtA and NitA, from Aspergillus nidulans [PDF]

open access: yes, 2009
Membrane proteins play an integral role in the control of ion transport across the cell membrane in biological systems. However, due to experimental constraints, structural and functional data available for these proteins is limited, especially ...
Symington, Vicki F.
core   +2 more sources

Emerging experimental and computational methods for studying redox‐regulated structural transitions

open access: yesFEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass   +2 more
wiley   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

Investigating the biosynthesis of heam d1 in pseudomonas aeruginosa: a cofactor for dissimilatory nitrite reductase. [PDF]

open access: yes, 2010
PhDHaem d1 is a modified tetrapyrrole unique to the periplasmic enzyme nitrite reductase where it acts in catalysing the reduction of nitrite (NO2 -) to nitric oxide (NO), as part of denitrification.
Parmar-Bhundia, Vina
core   +4 more sources

Sources of reducing equivalents for nitrate reductase in Pisum arvense roots

open access: yesActa Societatis Botanicorum Poloniae, 2014
The sources of the nitrate reductase (E.C 1.6.6.1) reducing equivalents were searched for in roots of Pisum arvense by measuring in vivo and in vitro nitrate reductase activity.
Grażyna Kłobus
doaj   +1 more source

Smaller is better: nanobodies meet NMR

open access: yesFEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Influence of lead on N03 uptake and reduction in cucumber seedlings

open access: yesActa Societatis Botanicorum Poloniae, 2014
The influence of PbCl2 on NO3- uptake and activity level of nitrate (NR) and nitrite MR) reductases in cotyledons and roots of cucumber seedlings was studied.
Marek Burzyński, Adam Grabowski
doaj   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

open access: yesFEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1 [PDF]

open access: yes, 2012
In this study, the flavin reductase (Fre) of Citrobatter freundii A1 was expressed in E. coli DH5a host with 1 mM IPTG induction. This recombinant protein was fused to 6xHis-tag, thus promising easier IMAC purification of the expressed enzyme.
Mamat, Nor Sasha Izana
core  

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