Results 51 to 60 of about 5,837 (209)

Comparison of the azoreductase and nitroreductase from Clostridium perfringens [PDF]

Applied and Environmental Microbiology, 1993
The purified azoreductase and nitroreductase of Clostridium perfringens, which have similar electrophoretic properties, both reacted in a Western blot (immunoblot) with a polyclonal antibody raised against the azoreductase. The activity of both enzymes was enhanced by flavin adenine dinucleotide and was inhibited by menadione, o-iodosobenzoic acid, and
F, Rafii, C E, Cerniglia
openaire   +2 more sources

Accessing active fragments for drug discovery utilising nitroreductase biocatalysis

Biocatalysis has played a limited role in the early stages of drug discovery. This is often attributed to the limited substrate scope of enzymes not affording access to vast areas of novel chemical space.
Eda, Yuce, Jóhannes, Reynisson, Gabriel, Oriomah, Lauren, Holder, Aimee, Jenkins, Sebastian, Cosgrove, Anja, Winter   +6 more
core   +2 more sources

tBid‐Mediated Genetic Ablation of Connective Tissue Cells Reveals Their Key Regulatory Function During Limb Regeneration in Axolotls

Advanced Science, EarlyView.
We establish a tBid‐mediated cell ablation system in axolotls, achieve rapid and efficient ablation of multiple cell types, including muscle stem cell, spinal cord cell, and connective tissue (CT) cells. We investigate the role of CT using tBid‐mediated CT ablation and identify its essential role for limb development and regeneration.
Yan Hu, Weimin Feng, Zitian He, Jiayi Zeng, Jingyi Tang, Shulin Li, Tongbo Yu, Sulei Fu, Hui Ma, Binbin Lu, Yanmei Liu, Ji‐Feng Fei   +11 more
wiley   +1 more source

Nitroreductase activity of heart lipoamide dehydrogenase [PDF]

Biochemical Journal, 1987
A novel reaction catalysed by lipoamide dehydrogenase is described. In the presence of NADH, lipoamide dehydrogenase reduces the nitro group of 4-nitropyridine and 4-nitropyridine N-oxide. The elution profiles from a DEAE-cellulose column for the dehydrogenase and nitroreductase activities are identical.
openaire   +2 more sources

Pathogen‐Centric Activation of an Azoreductase‐Responsive Antibody‐Antibiotic Conjugate for the Targeted Eradication of MRSA

Advanced Science, EarlyView.
A pathogen‐centric antibody‐antibiotic conjugate (AZO‐AAC) releases antibiotics via bacterial azoreductase, independent of host‐lysosomal function. This mechanism achieves nanomolar eradication of both planktonic and intracellular methicillin‐resistant Staphylococcus aureus (MRSA).
Qi Cheng, Lianqi Liu, Chenghua Liu, Fei Xie, Jingwen Dong, Xiaoyu Qin, Shunxiang Huang, Xian Li, Xingyuan Kou, Hongbin Deng, Jiannan Feng, Wu Zhong, Dian Xiao, Xinbo Zhou   +13 more
wiley   +1 more source

Design, Synthesis, and Evaluation of Doxifluridine Derivatives as Nitroreductase-Responsive Anticancer Prodrugs

Molecules
Antimetabolite antitumor drugs interfere with nucleic acid and DNA synthesis, causing cancer cell death. However, they also affect rapidly dividing normal cells and cause serious side effects.
Xinmeng Zhang, Taimin Dong, Xu Li, Changjie Xu, Fanghui Chen, Shiben Wang, Xuekun Wang   +6 more
doaj   +1 more source

Therapeutic Applications of Stimuli‐Based Release and Engineering of Extracellular Vesicles

Advanced NanoBiomed Research, EarlyView.
This review summarizes the effects of endogenous and exogenous stimuli, their effects on the natural release of extracellular vesicles, as well as their uptake and release. It also gives an overview of stimuli‐responsive EVs and their therapeutic applications. Extracellular vesicles (EVs), nano‐ to microsized lipid bilayer membrane‐bound particles, are
Gloria Kemunto, Kristen Dellinger
wiley   +1 more source

Cell-Specific Chemical Delivery Using a Selective Nitroreductase–Nitroaryl Pair

, 2018
The utility of small molecules to probe or perturb biological systems is limited by the lack of cell-specificity. ‘Masking’ the activity of small molecules using a general chemical modification and ‘unmasking’ it only within target cells could overcome ...
Michael R., Tadross, Luke D., Lavis, Jonathan B., Grimm, Laura M., Wysocki, Zev J., Gartner, Todd D., Gruber, Chithra, Krishnamurthy   +6 more
core   +1 more source

Nitroreduction of flutamide by Cunninghamella elegans NADPH: Cytochrome P450 reductase

Biochemistry and Biophysics Reports, 2022
The microbial model of mammalian drug metabolism, Cunninghamella elegans, has three cytochrome P450 reductase genes in its genome: g1631 (CPR_A), g4301 (CPR_B), and g7609 (CPR_C).
Mohd Faheem Khan, Cormac D. Murphy
doaj   +1 more source

Environmental Identification of Novel Enzymes for Polyurethane and Polyamide Degradation

Angewandte Chemie International Edition, EarlyView.
Heteroatom‐containing plastics such as polyurethanes and nylons are produced at scale yet remain largely unrecycled. Biocatalysts offer a promising route to depolymerization under mild conditions. Here, we present a screening platform for the discovery of novel polyurethane‐ and nylon‐degrading enzymes from environmental samples and demonstrate ...
Malthe Kjær Bendtsen, Andreas Møllebjerg, Samuel Peña‐Díaz, Rosie Graham, Nicolai Claus Petersen, Bjørk Nolsøe Isaksen, Mathias Carstensen, Martin B. Johansen, Andreas Sommerfeldt, Allan R. Petersen, Iddi Khamisi Chuma, Cecilie Ryberg, Thomas Rea Wittenborn, Virginia Gichuru, Huabing Wang, Carsten Scavenius, Alexander Sandahl, Daniel E. Otzen   +17 more
wiley   +1 more source