Results 191 to 200 of about 18,603 (231)

The effect of detergents on the basicity of nitrotyrosine in proteins

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1974
The effects of sodium dodecylsulfate, Triton X-100 and hexadecyltrimethylammonium bromide, an anionic, neutral, and cationic detergent, respectively on the pKa of nitrotyrosine in bovine serum albumin and hemerythrin have been studied in the denatured state where a possible concomitant conformational change is less likely to contribute to the observed ...
J L, York, M P, Roberts
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Nitrotyrosine Residues in Placenta

Hypertension, 1996
The interaction of nitric oxide and superoxide produces peroxynitrite anion, a strong, long-lived oxidant with pronounced deleterious effects that may cause vascular damage. The formation and action of peroxynitrite can be detected by immunohistochemical localization of nitrotyrosine residues.
L, Myatt, R B, Rosenfield, A L, Eis, D E, Brockman, I, Greer, F, Lyall   +5 more
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A review of approaches to the analysis of 3-nitrotyrosine

Amino Acids, 2003
Our understanding of in vivo tyrosine nitration has been confounded by problems associated with the analytical approaches that have been employed to quantify 3-nitroyrosine (3-NT). Trace analysis is a demanding task under the best of circumstances, but 3-NT offers some special concerns.
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Loss of 3-Nitrotyrosine on Exposure to Hypochlorous Acid: Implications for the Use of 3-Nitrotyrosine as a Bio-markerin Vivo

Biochemical and Biophysical Research Communications, 1999
Peroxynitrite (ONOO-) is a reactive nitrogen species which in vivo is often assessed by the measurement of free or protein bound 3-nitrotyrosine. Indeed, 3-nitrotyrosine has been detected in many human diseases. However, at sites of inflammation there is also production of the powerful oxidant hypochlorous acid (HOCl) formed by the enzyme ...
M, Whiteman, B, Halliwell
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Plastocyanin conformation. The effect of nitrotyrosine modification and pH

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
Plastocyanin isolated from several species including spinach, poplar, and lettuce showed conformational changes both upon reduction and upon lowering the pH as determined by near-ultraviolet absorption and fluorescence measurements. The fluorescence excitation maximum was at 278 nm for all species of plastocyanin measured.
E L, Gross, G P, Anderson, S L, Ketchner, J E, Draheim   +3 more
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Enzymatic Reduction of 3-Nitrotyrosine Generates Superoxide

Chemical Research in Toxicology, 1998
Spin-trapping with 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) was used to demonstrate that 3-nitrotyrosine (nitrotyrosine) promotes the formation of substantial amounts of reactive oxygen species (O2.- and *OH), when incubated with NAD(H)-cytochrome c reductase and a corresponding electron donor. Spin adduct formation is strongly inhibited by the presence
A G, Krainev, T D, Williams, D J, Bigelow   +2 more
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Chemical labeling and enrichment of nitrotyrosine-containing peptides

Talanta, 2010
Protein tyrosine nitration (PTN) is a post-translational modification of proteins associated with a number of inflammatory diseases. While PTN is rather selective (not all proteins are modified and within a protein, only certain tyrosines are subject to nitration), no consensus sequence has been identified.
Abello, Nicolas, Barroso, Begona, Kerstjens, Huib A. M., Postma, Dirkje S., Bischoff, Rainer   +4 more
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Do Human Atherosclerotic Lesions Contain Nitrotyrosine?

Biochemical and Biophysical Research Communications, 1996
Nitrotyrosine has been widely used as a marker of peroxynitrite formation in normal and diseased tissues. However, studies of normal human aortic intima and atherosclerotic lesions at various stages of development failed to reveal its presence except in a very few specimens of both normal and diseased tissues.
P, Evans, H, Kaur, M J, Mitchinson, B, Halliwell   +3 more
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Homoarginine and 3-nitrotyrosine in patients with takotsubo cardiomyopathy

International Journal of Cardiology, 2014
In recent years, homoargininewas shown to be a cardiovascular risk factor [1], and to herald a poor prognosis in heart failure patients [2]. Yet, the underlying mechanism is elusive. Human and animal studies suggest that the enzyme responsible for the biosynthesis of homoarginine is arginine:glycine amidinotransferase (AGAT) [3–5].
Arslan Arinc Kayacelebi, Thanh Ha Nguyen, Chris Neil, John D. Horowitz, Jens Jordan, Dimitrios Tsikas   +5 more
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Localization of 3‐nitrotyrosine to rheumatoid and normal synovium

Arthritis & Rheumatism, 2001
To determine the localization of 3-nitrotyrosine (3-NT), a footprint marker of peroxynitrite (ONOO-) and other reactive nitrogen species, to the inflamed human synovium and to compare this with normal synovial and nonsynovial tissue of human and animal origin.Monoclonal and polyclonal antibodies were used to investigate for 3-NT, inducible nitric oxide
P I, Mapp, R, Klocke, D A, Walsh, J K, Chana, C R, Stevens, P J, Gallagher, D R, Blake   +6 more
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