A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency
Communications Biology, 2023 The nek4, eIK1, and eIK2 sensor kinases play a key role in Plasmodium amino acid sensing, enabling these parasites to fine-tune replication and development in response to amino acid availability.
Inês M. Marreiros +9 more
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Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota [PDF]
Nature Communications, 2020 Bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with flow cytometry (FACS) has been utilized to profile active microbiome of environmental samples.
Talia D. Valentini +6 more
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Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis [PDF]
Frontiers in Microbiology, 2020 BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Katherine F. Steward +14 more
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Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation [PDF]
eLife, 2020 The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-
Harrison Tudor Evans +2 more
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All-atom protein sequence design using discrete diffusion models [PDF]
Journal of CheminformaticsAdvancing protein design is crucial for breakthroughs in medicine and biotechnology. Traditional approaches for protein sequence representation often rely solely on the 20 canonical amino acids, limiting the representation of non-canonical amino acids ...
Amelia Villegas-Morcillo +3 more
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A computational approach for designing D-proteins with non-canonical amino acid optimised binding affinity. [PDF]
PLoS ONE, 2017 Redesigning protein surface topology to improve target binding holds great promise in the search for highly selective therapeutics. While significant binding improvements can be achieved using natural amino acids, the introduction of non-canonical ...
Michael Garton +2 more
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Mechanistic studies of non-canonical amino acid mutagenesis. [PDF]
Methods Enzymol, 2021 AbstractOver the past decade, harnessing the cellular protein synthesis machinery to incorporate non-canonical amino acids (ncAAs) into tailor-made peptides has significantly advanced many aspects of molecular science. More recently, groundbreaking progress in our ability to engineer this machinery for improved ncAA incorporation has led to significant
Fleisher RC, Michael N, Gonzalez RL.
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Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives [PDF]
Frontiers in Molecular BiosciencesIntroductionPyrrolysyl-tRNA synthetase (PylRS) is a key enzyme for the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. However, its native form has a limited substrate scope.
Anastasia Dakhnevich +4 more
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Co-translational Installation of Posttranslational Modifications by Non-canonical Amino Acid Mutagenesis. [PDF]
Chembiochem, 2023 AbstractProtein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co‐translational installation and studies of PTMs through noncanonical amino acid (ncAA) mutagenesis.
Niu W, Guo J.
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An in vivo photo-cross-linking approach reveals a homodimerization domain of Aha1 in S. cerevisiae. [PDF]
PLoS ONE, 2014 Protein-protein interactions play an essential role in almost any biological processes. Therefore, there is a particular need for methods which describe the interactions of a defined target protein in its physiological context. Here we report a method to
Michael Berg +4 more
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