Results 21 to 30 of about 262,938 (280)
The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei. [PDF]
PLoS ONE, 2009 The carboxy-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. The canonical CTD is characterized by heptapeptide repeats.
Anish Das, Vivian Bellofatto
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Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases. [PDF]
Chembiochem, 2022 AbstractIn this comprehensive review, I focus on the twenty E. coli aminoacyl‐tRNA synthetases and their ability to charge non‐canonical amino acids (ncAAs) onto tRNAs. The promiscuity of these enzymes has been harnessed for diverse applications including understanding and engineering of protein function, creation of organisms with an expanded genetic ...
Hartman MCT.
europepmc +3 more sources
STAR Protocols, 2023 Summary: Bioorthogonal labeling and click chemistry techniques allow the detailed examination of cellular physiology through tagging and visualizing newly synthesized proteins.
Alison Keolani Carlisle +2 more
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Harnessing Transaminases to Construct Azacyclic Non-Canonical Amino Acids
Nature Synthesis, 2023 Non-canonical amino acids (ncAAs) are prized building blocks in the synthesis of natural products, designer peptides and drug molecules. Despite their general utility, the complex structure of these molecules still presents an enormous challenge for chemical synthesis. Here, we develop a one-pot chemoenzymatic approach for the construction of azacyclic
Tsung-Han Chao +4 more
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Using Genetic Code Expansion for Protein Biochemical Studies
Frontiers in Bioengineering and Biotechnology, 2020 Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung +3 more
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Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids [PDF]
Journal of the American Chemical Society, 2017 Nisin is a complex lanthipeptide that has broad spectrum antibacterial activity. In efforts to broaden the structural diversity of this ribosomally synthesized lantibiotic, we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids (ncAAs) at discrete positions.
Claudio Zambaldo +3 more
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Nature Communications, 2023 The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan +13 more
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Incorporation of Non-Canonical Amino Acids [PDF]
, 2015 In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical ...
Lilia, Leisle +3 more
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A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process
Crystals, 2021 L-proline amide hydrolase (PAH, EC 3.5.1.101) is a barely described enzyme belonging to the peptidase S33 family, and is highly similar to prolyl aminopeptidases (PAP, EC. 3.4.11.5).
Sergio Martinez-Rodríguez +4 more
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Influence of Sulfur-Containing Diamino Acid Structure on Covalently Crosslinked Copolypeptide Hydrogels. [PDF]
, 2018 Biologically occurring non-canonical di-α-amino acids were converted into new di-N-carboxyanhydride (di-NCA) monomers in reasonable yields with high purity.
Deming, Timothy J +4 more
core +1 more source