Results 41 to 50 of about 262,938 (280)

The Endoplasmic Reticulum Glucosyltransferase Recognizes Nearly Native Glycoprotein Folding Intermediates [PDF]

, 2004
The UDP-Glc:glycoprotein glucosyltransferase (GT), a key player in the endoplasmic reticulum (ER) quality control of glycoprotein folding, only glucosylates glycoproteins displaying non-native conformations.
Caramelo, Julio Javier, Castro, Olga Alejandra, de Prat Gay, Gonzalo, Parodi, Armando José A.   +3 more
core   +1 more source

Extensive breaking of genetic code degeneracy with non-canonical amino acids

Nature Communications, 2023
AbstractGenetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation. One type of GCE, sense codon reassignment (SCR), focuses on breaking the degeneracy of the 61 sense codons which encode for only 20 amino acids.
Clinton A. L. McFeely, Bipasana Shakya, Chelsea A. Makovsky, Aidan K. Haney, T. Ashton Cropp, Matthew C. T. Hartman   +5 more
openaire   +3 more sources

Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
Synthetic biology holds promise to revolutionize the life sciences and biomedicine via expansion of macromolecular diversity outside the natural chemical space. Use of non-canonical amino acids (ncAAs) via codon reassignment has found diverse applications in protein structure and interaction analysis, introduction of post-translational modifications ...
Cui, Zhenling, Johnston, Wayne A., Alexandrov, Kirill   +2 more
openaire   +4 more sources

Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids

Frontiers in Bioengineering and Biotechnology, 2020
The 22 genetically encoded amino acids (AAs) present in proteins (the 20 standard AAs together with selenocysteine and pyrrolysine), are commonly referred as proteinogenic AAs in the literature due to their appearance in ribosome-synthetized polypeptides.
Sergio Martínez-Rodríguez, Jesus M. Torres, Pilar Sánchez, Esperanza Ortega   +3 more
doaj   +1 more source

An Alternative Model of Amino Acid Replacement [PDF]

, 2004
The observed correlations between pairs of homologous protein sequences are typically explained in terms of a Markovian dynamic of amino acid substitution.
Altschul, Altschul, Bennet, Brenner, Brenner, Bruno, Crooks, Crooks, Felsenstein, Goldman, Goldman, Gonnet, Henikoff, Henikoff, Jones, Koshi, Marti-Renom, Muller, Murzin, M  ller, Park, Schneider, Sjolander, Smith, Thorne, Topham, Yona, Zachariah   +27 more
core   +3 more sources

Development of Multivalent Conjugates with a Single Non‐Canonical Amino Acid

ChemBioChem, 2023
AbstractProteins represent powerful biomacromolecules due to their unique functionality and broad utility both in the cell and in non‐biological applications. The genetic encoding of non‐canonical amino acids (ncAAs) facilitates functional diversification of these already powerful proteins.
Naya K. Burrow, Robert K. Gourdie, Elizabeth A. King, Christopher R. Travis, Cameron M. Goff, Zachary M. Nimmo, Justin M. Berg, Emily L. Boyt, Douglas D. Young   +8 more
openaire   +2 more sources

Detecting Repetitions and Periodicities in Proteins by Tiling the Structural Space [PDF]

, 2013
The notion of energy landscapes provides conceptual tools for understanding the complexities of protein folding and function. Energy Landscape Theory indicates that it is much easier to find sequences that satisfy the "Principle of Minimal Frustration ...
Espada, Rocío, Ferreiro, Diego U., Parra, R. Gonzalo, Sippl, Manfred J., Sánchez, Ignacio E.   +4 more
core   +2 more sources

Initiation of Protein Synthesis with Non‐Canonical Amino Acids In Vivo

Angewandte Chemie International Edition, 2020
AbstractBy transplanting identity elements into E. coli tRNAfMet, we have engineered an orthogonal initiator tRNA (itRNATy2) that is a substrate for Methanocaldococcus jannaschii TyrRS. We demonstrate that itRNATy2 can initiate translation in vivo with aromatic non‐canonical amino acids (ncAAs) bearing diverse sidechains.
Jeffery M. Tharp, Omer Ad, Kazuaki Amikura, Fred R. Ward, Emma M. Garcia, Jamie H. D. Cate, Alanna Schepartz, Dieter Söll   +7 more
openaire   +6 more sources

Non-Canonical Amino Acids in Analyses of Protease Structure and Function

International Journal of Molecular Sciences, 2023
All known organisms encode 20 canonical amino acids by base triplets in the genetic code. The cellular translational machinery produces proteins consisting mainly of these amino acids. Several hundred natural amino acids serve important functions in metabolism, as scaffold molecules, and in signal transduction.
Peter Goettig, Nikolaj G. Koch, Nediljko Budisa   +2 more
openaire   +2 more sources

Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry [PDF]

Biomolecules, 2020
Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids that carry a handle for click chemistry in addition to the photo-crosslinker.
openaire   +4 more sources