Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Synthetic biology holds promise to revolutionize the life sciences and biomedicine via expansion of macromolecular diversity outside the natural chemical space.
Zhenling Cui +2 more
doaj +2 more sources
Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins [PDF]
Molecules, 2023 The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals.
Liang Chen +6 more
doaj +2 more sources
Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry [PDF]
Biomolecules, 2020 Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids.
Jan-Erik Hoffmann
doaj +2 more sources
Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction [PDF]
Bioresources and BioprocessingThe use of enzymes to catalyze Henry reaction has advantages of mild reaction conditions and low contamination, but low enzyme activity of promiscuous catalysis limits its application.
Lan Wang +8 more
doaj +2 more sources
Dual incorporation of non-canonical amino acids enables production of post-translationally modified selenoproteins [PDF]
Frontiers in Molecular Biosciences, 2023 Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to ...
Pearl Morosky +8 more
doaj +2 more sources
Microbial production of non‐canonical amino acids [PDF]
Microbial Biotechnology, 2023 Lawrence P. Wackett
doaj +2 more sources
Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons [PDF]
Nature Communications, 2021 Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and localise ...
Diogo Bessa-Neto +9 more
doaj +2 more sources
Editorial: Exploring and expanding the protein universe with non-canonical amino acids [PDF]
Frontiers in Molecular Biosciences, 2023 Gustavo Fuertes +2 more
doaj +2 more sources
Through bonds or contacts? Mapping protein vibrational energy transfer using non-canonical amino acids [PDF]
Nature Communications, 2021 Vibrational energy transfer (VET) is essential for protein function as it is responsible for efficient energy dissipation in reaction sites and is linked to pathways of allosteric communication.
Erhan Deniz +10 more
doaj +2 more sources
A computational approach for designing D-proteins with non-canonical amino acid optimised binding affinity. [PDF]
PLoS ONE, 2017 Redesigning protein surface topology to improve target binding holds great promise in the search for highly selective therapeutics. While significant binding improvements can be achieved using natural amino acids, the introduction of non-canonical ...
Michael Garton +2 more
doaj +1 more source