Results 41 to 50 of about 11,400,092 (255)
The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei. [PDF]
PLoS ONE, 2009 The carboxy-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. The canonical CTD is characterized by heptapeptide repeats.
Anish Das, Vivian Bellofatto
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Microorganisms, 2021 Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low oxygen feeding zone with the accumulation of mixed acid fermentation products ...
Ángel Córcoles García +2 more
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Xenogeneic, extracorporeal liver perfusion in primates improves the ratio of branched-chain amino acids to aromatic amino acids (Fischer's ratio) [PDF]
, 1999 In fulminant hepatic failure (FHF), the development of hepatic encephalopathy is associated with grossly abnormal concentrations of plasma amino acids (PAA).
Puhl, G. +7 more
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Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids [PDF]
Journal of the American Chemical Society, 2017 Nisin is a complex lanthipeptide that has broad spectrum antibacterial activity. In efforts to broaden the structural diversity of this ribosomally synthesized lantibiotic, we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids (ncAAs) at discrete positions.
Claudio Zambaldo +3 more
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Scientific Reports, 2023 Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution.
Jeffrey L. Schloßhauer +5 more
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Phase-separation propensity of non-ionic amino acids in peptide-based complex coacervation systems [PDF]
, 2022 Uncovering the sequence-encoded molecular grammar that governs the liquid–liquid phase separation (LLPS) of proteins is a crucial issue to understand dynamic compartmentalization in living cells and the emergence of protocells. Here, we present a model
Kentaro, Shiraki +6 more
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Development of Multivalent Conjugates with a Single Non‐Canonical Amino Acid
ChemBioChem, 2023 AbstractProteins represent powerful biomacromolecules due to their unique functionality and broad utility both in the cell and in non‐biological applications. The genetic encoding of non‐canonical amino acids (ncAAs) facilitates functional diversification of these already powerful proteins.
Naya K. Burrow +8 more
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The genetic incorporation of thirteen novel non-canonical amino acids [PDF]
Chem. Commun., 2014 Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase–tRNAPylCUA pair.
Alfred, Tuley +5 more
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Molecules, 2014 In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code.
Ying Ma +4 more
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Expanding the genetic code of Salmonella with non-canonical amino acids [PDF]
Scientific Reports, 2016 AbstractThe diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few
Qinglei Gan +3 more
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