Results 31 to 40 of about 11,400,092 (255)

Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering

Results in Engineering
Non-canonical amino acids (ncAAs), as derivatives of canonical amino acids (cAAs), when specifically incorporated into proteins, could enrich protein functions.
Zhen Hou, Junkai Tuo, Xiaoyan Ma, Yi-Xin Huo   +3 more
doaj   +3 more sources

Mechanistic studies of non-canonical amino acid mutagenesis [PDF]

, 2021
Abstract Over the past decade, harnessing the cellular protein synthesis machinery to incorporate non-canonical amino acids (ncAAs) into tailor-made peptides has significantly advanced many aspects of molecular science. More recently, groundbreaking progress in our ability to engineer this machinery for improved ncAA incorporation has ...
Rachel C. Fleisher, Nina Michael, Ruben L. Gonzalez   +2 more
openaire   +2 more sources

A computational approach for designing D-proteins with non-canonical amino acid optimised binding affinity. [PDF]

PLoS ONE, 2017
Redesigning protein surface topology to improve target binding holds great promise in the search for highly selective therapeutics. While significant binding improvements can be achieved using natural amino acids, the introduction of non-canonical ...
Michael Garton, Maryam Sayadi, Philip M Kim   +2 more
doaj   +1 more source

Engineering Pyrrolysyl-tRNA Synthetase for the Incorporation of Non-Canonical Amino Acids with Smaller Side Chains [PDF]

International Journal of Molecular Sciences, 2021
Juri Rappsilber, Nediljko Budisa, Peter Goettig   +2 more
exaly   +2 more sources

One-pot chemoenzymatic syntheses of non-canonical amino acids. [PDF]

J Ind Microbiol Biotechnol
Abstract   Despite their prevalent use in drug discovery and protein biochemistry, non-canonical amino acids are still challenging to synthesize through purely chemical means. In recent years, biocatalysis has emerged as a transformative paradigm for small-molecule synthesis.
Chao TH, Wu X, Renata H.
europepmc   +3 more sources

Non-canonical amino acid labeling in proteomics and biotechnology [PDF]

Journal of Biological Engineering, 2019
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries. Labeled proteins can then be selectively conjugated to fluorophores, affinity reagents, peptides, polymers, nanoparticles or ...
Aya M. Saleh, Kristen M. Wilding, Sarah Calve, Bradley C. Bundy, Tamara L. Kinzer-Ursem   +4 more
openaire   +3 more sources

Using Genetic Code Expansion for Protein Biochemical Studies

Frontiers in Bioengineering and Biotechnology, 2020
Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung, Kazuaki Amikura, Dieter Söll, Dieter Söll   +3 more
doaj   +1 more source

Incorporation of Non-Canonical Amino Acids [PDF]

, 2015
In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical ...
Lilia, Leisle, Francis, Valiyaveetil, Ryan A, Mehl, Christopher A, Ahern   +3 more
openaire   +2 more sources

A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids

Heliyon, 2020
The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids
Ximena Steinberg, Jason Galpin, Gibran Nasir, Romina V. Sepúlveda, Ernesto Ladron de Guevara, Fernando Gonzalez-Nilo, Leon D. Islas, Christopher A. Ahern, Sebastian E. Brauchi   +8 more
doaj   +1 more source

Synthesis of non-canonical amino acids through dehydrogenative tailoring. [PDF]

Nature
Amino acids are essential building blocks in biology and chemistry. Whereas nature relies on a small number of amino acid structures, chemists desire access to a vast range of structurally diverse analogues1-3. The selective modification of amino acid side-chain residues represents an efficient strategy to access non-canonical derivatives of value in ...
Gu X, Zhang YA, Zhang S, Wang L, Ye X, Occhialini G, Barbour J, Pentelute BL, Wendlandt AE.   +8 more
europepmc   +3 more sources