Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins [PDF]
Molecules, 2023 The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals.
Liang Chen +6 more
doaj +2 more sources
Engineering Protease-Resistant Peptides via Non-Canonical Amino Acids: Design Strategies and Biosynthetic Advances [PDF]
BioengineeringPeptide therapeutics offer high target selectivity and low toxicity, but their clinical utility remains constrained by rapid proteolysis in vivo and negligible oral bioavailability.
Chen Deng +5 more
doaj +2 more sources
Dual incorporation of non-canonical amino acids enables production of post-translationally modified selenoproteins [PDF]
Frontiers in Molecular Biosciences, 2023 Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to ...
Pearl Morosky +8 more
doaj +2 more sources
Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The 22 genetically encoded amino acids (AAs) present in proteins (the 20 standard AAs together with selenocysteine and pyrrolysine), are commonly referred as proteinogenic AAs in the literature due to their appearance in ribosome-synthetized polypeptides.
Sergio Martínez-Rodríguez +3 more
doaj +4 more sources
Non-Canonical Amino Acid-Based Engineering of (R)-Amine Transaminase [PDF]
Frontiers in Chemistry, 2022 Non-canonical amino acids (ncAAs) have been utilized as an invaluable tool for modulating the active site of the enzymes, probing the complex enzyme mechanisms, improving catalytic activity, and designing new to nature enzymes.
Amol D. Pagar +8 more
doaj +3 more sources
Enzymes with Non-Canonical Amino Acids
Enzyme design and engineering strategies rely almost exclusively on Nature’s alphabet of twenty canonical amino acids. Recent years have seen the emergence of powerful genetic code expansion methods that allow hundreds of structurally diverse amino acids
Burke, Ashleigh +2 more
core +10 more sources
Non‐Canonical Amino Acids in Protein Polymer Design
Polymer Reviews, 2007 Protein polymers can be prepared with essentially absolute control of chain length, sequence, and stereochemistry through biological synthesis in microbial hosts.
Connor, Rebecca E., Tirrell, David A.
core +3 more sources
Beyond 20 in the 21st Century: Prospects and Challenges of Non-canonical Amino Acids in Peptide Drug Discovery [PDF]
ACS Medicinal Chemistry Letters, 2023 Danielle Schultz +2 more
exaly +2 more sources
Parameterizing non-canonical amino acids for cyclic peptide simulations. [PDF]
Methods EnzymolNon-canonical amino acids (ncAAs) are increasingly used to expand the functional chemical space available to biomolecular design. They are particularly useful for cyclic peptides, which have smaller interaction surfaces than proteins and thus require precisely tuned high-affinity contacts with very specific chemical motifs to achieve target affinity ...
Wijesiri NK, Brown BP.
europepmc +4 more sources
Non-canonical amino acids as a tool for the thermal stabilization of enzymes. [PDF]
Protein Eng Des Sel, 2023 AbstractBiocatalysis has become a powerful alternative for green chemistry. Expanding the range of amino acids used in protein biosynthesis can improve industrially appealing properties such as enantioselectivity, activity and stability. This review will specifically delve into the thermal stability improvements that non-canonical amino acids (ncAAs ...
Lugtenburg T +2 more
europepmc +4 more sources