Using Genetic Code Expansion for Protein Biochemical Studies
Frontiers in Bioengineering and Biotechnology, 2020 Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung +3 more
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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids [PDF]
, 2018 Nature has a variety of possibilities to create new protein functions by modifying the sequence of the individual amino acid building blocks. However, all variations are based on the 20 canonical amino acids (cAAs).
Bartholomae, Maike +6 more
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A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids
Heliyon, 2020 The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids
Ximena Steinberg +8 more
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The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei. [PDF]
PLoS ONE, 2009 The carboxy-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. The canonical CTD is characterized by heptapeptide repeats.
Anish Das, Vivian Bellofatto
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Microorganisms, 2021 Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low oxygen feeding zone with the accumulation of mixed acid fermentation products ...
Ángel Córcoles García +2 more
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Influence of Sulfur-Containing Diamino Acid Structure on Covalently Crosslinked Copolypeptide Hydrogels. [PDF]
, 2018 Biologically occurring non-canonical di-α-amino acids were converted into new di-N-carboxyanhydride (di-NCA) monomers in reasonable yields with high purity.
Deming, Timothy J +4 more
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Bacterial riboproteogenomics : the era of N-terminal proteoform existence revealed [PDF]
, 2020 With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are ...
Fijalkowska, Daria +3 more
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Bioprocess Development for Lantibiotic Ruminococcin-A Production in Escherichia coli and Kinetic Insights Into LanM Enzymes Catalysis [PDF]
, 2019 Ruminococcin-A (RumA) is a peptide antibiotic with post-translational modifications including thioether cross-links formed from non-canonical amino acids, called lanthionines, synthesized by a dedicated lanthionine-generating enzyme RumM.
Adrian, Lorenz +5 more
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Scientific Reports, 2023 Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution.
Jeffrey L. Schloßhauer +5 more
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Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments [PDF]
, 2013 The incorporation of several non-canonical amino acids into the Thermoanaerobacter thermohydrosulfuricus lipase confers not only activity enhancement upon treatment with organic solvents (by up to 450%) and surfactants (resp.
Acevedo-Rocha, Carlos G. +7 more
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