Results 151 to 160 of about 18,387,865 (333)
Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation. [PDF]
, 2012 The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human
Savitsky, P +40 more
core +1 more source
Viruses, 2017 Viruses have evolved unique strategies to evade or subvert autophagy machinery. Enterovirus A71 (EV-A71) induces autophagy during infection in vitro and in vivo.
J. Lai +6 more
semanticscholar +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Non-structural Proteins in Newcastle Disease Virus-infected Cells
Journal of General Virology, 1982 Examination of pulse-labelled Newcastle disease virus (NDV)-infected chick embryo fibroblasts (CEF) by two-dimensional polyacrylamide gel electrophoresis revealed the presence of two-virus-coded non-structural polypeptides of mol. wt. 36K and 33K. Longer pulses and pulse-chase incubations revealed the production of an additional, glycosylated, non ...
P, Chambers, A C, Samson
openaire +2 more sources
Scientific Reports, 2017 Chikungunya virus (CHIKV), a mosquito-borne alphavirus, causes febrile disease, muscle and joint pain, which can become chronic in some individuals. The non-structural protein 3 (nsP3) plays essential roles during infection, but a complete understanding ...
Roland Remenyi +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Frontiers in Microbiology, 2017 Non-structural protein 7 (NSP7), which can be further cleaved into NSP7α and NSP7β, is one of the most conserved proteins of porcine reproductive and respiratory syndrome virus (PRRSV).
Jiaping Chen +7 more
semanticscholar +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Structural determinants for protein adsorption/non-adsorption to silica surface.
PLoS ONE, 2013 The understanding of the mechanisms involved in the interaction of proteins with inorganic surfaces is of major interest in both fundamental research and applications such as nanotechnology.
Christelle Mathé +10 more
doaj +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source