An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET [PDF]
eLife, 2021 With the recent explosion in high-resolution protein structures, one of the next frontiers in biology is elucidating the mechanisms by which conformational rearrangements in proteins are regulated to meet the needs of cells under changing conditions ...
William N Zagotta +9 more
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Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation [PDF]
Nature Communications, 2022 Incorporation of noncanonical amino acids into proteins holds great promise for altering structure and function of these proteins. Here the authors generate metabolically modified prokaryotic and eukaryotic cells that can biosynthesize sTyr and ...
Yuda Chen +10 more
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CENCAT enables immunometabolic profiling by measuring protein synthesis via bioorthogonal noncanonical amino acid tagging [PDF]
Cell Reports: MethodsSummary: Cellular energy metabolism significantly contributes to immune cell function. To further advance immunometabolic research, novel methods to study the metabolism of immune cells in complex samples are required. Here, we introduce CENCAT (cellular
Frank Vrieling +9 more
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Noncanonical amino acid-aided synthesis of anti-PD-L1 bispecific nanobody for colon cancer immunotherapy [PDF]
Communications BiologyColorectal cancer (CRC) is the second leading cause of cancer-related mortality, and the incidence of early-onset colon cancer has been increasing globally in recent years. The development of immunotherapies for colon cancer is critical for providing new
Xinyu Hu +13 more
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A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli [PDF]
Nature CommunicationsGenetic code expansion (GCE) has significantly enhanced the diversity of proteins in the biological world, leading to a wide range of applications.
Jingxuan Zhang +8 more
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Computationally Assisted Noncanonical Amino Acid Incorporation [PDF]
ACS Central ScienceChengzhu Fang +5 more
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New Additions to the Arsenal of Biocatalysts for Noncanonical Amino Acid Synthesis. [PDF]
Curr Opin Green Sustain Chem, 2022 Alfonzo E, Das A, Arnold FH.
europepmc +2 more sources
Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation. [PDF]
Chem RevMajekodunmi T, Britton D, Montclare JK.
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Misincorporation Proteomics Technologies: A Review
Proteomes, 2021 Proteinopathies are diseases caused by factors that affect proteoform conformation. As such, a prevalent hypothesis is that the misincorporation of noncanonical amino acids into a proteoform results in detrimental structures.
Joel R. Steele +6 more
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Enhancing Metal-binding with Noncanonical Coordinating Amino Acids
CHIMIA, 2021 More than 50% of proteinogenic amino acid sidechains can bind metal ions, enabling proteins and peptides to bear these ions as cofactors. Nevertheless, post-translational modifications and incorporation of noncanonical amino acids bestow peptides and ...
Luca Sauser, Michal S. Shoshan
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