Results 21 to 30 of about 2,835,571 (143)

Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice

Molecular Therapy: Methods & Clinical Development, 2023
Gene therapy using recombinant adeno-associated virus (rAAV) relies on safe, efficient, and precise in vivo gene delivery that is largely dependent on the AAV capsid.
Hao Chang, Ailing Du, Jun Jiang, Lingzhi Ren, Nan Liu, Xuntao Zhou, Jialing Liang, Guangping Gao, Dan Wang   +8 more
doaj   +1 more source

A Diene-Containing Noncanonical Amino Acid Enables Dual Functionality in Proteins: Rapid Diels-Alder Reaction with Maleimide or Proximity-Based Dimerization. [PDF]

Angew Chem Int Ed Engl, 2019
St Amant AH, Huang F, Lin J, Rickert K, Oganesyan V, Lemen D, Mao S, Harper J, Marelli M, Wu H, Gao C, Read de Alaniz J, Christie RJ.   +12 more
europepmc   +2 more sources

Tsc3 regulates SPT amino acid choice in Saccharomyces cerevisiae by promoting alanine in the sphingolipid pathway

Journal of Lipid Research, 2018
The generation of most sphingolipids (SPLs) starts with condensation between serine and an activated long-chain fatty acid catalyzed by serine palmitoyltransferase (SPT).
Jihui Ren, Essa M. Saied, Aaron Zhong, Justin Snider, Christian Ruiz, Christoph Arenz, Lina M. Obeid, Geoffrey D. Girnun, Yusuf A. Hannun   +8 more
doaj   +1 more source

A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality

Frontiers in Bioengineering and Biotechnology, 2022
Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties.
Simon K. Krebs, Simon K. Krebs, Nathanaël Rakotoarinoro, Nathanaël Rakotoarinoro, Marlitt Stech, Anne Zemella, Stefan Kubick, Stefan Kubick, Stefan Kubick   +8 more
doaj   +1 more source

Engineering a light-controllable fluorescent protein for peroxynitrite detection via genetic code expansion [PDF]

RSC Chemical Biology
We report a noncanonical amino acid-containing photoconvertible fluorescent protein for selective peroxynitrite detection. Incorporation of p-borono-l-phenylalanine into circularly permuted mEos2 affords a peroxynitrite-responsive sensor that retains ...
Gloricelly M. Roman Arocho, Parthasarathi Das, Sachin C. Tennakoon, Jared B. Shaw, Wei Niu, Jiantao Guo   +5 more
doaj   +1 more source

Diel rhythmicity in amino acid uptake by Prochlorococcus [PDF]

, 2008
The marine cyanobacterium Prochlorococcus, the most abundant phototrophic organism on Earth, numerically dominates the phytoplankton in nitrogen (N)-depleted oceanic gyres.
Terry, Matthew J, Garczarek, Laurence, Tarran, Glen A, Mary, Isabelle, Burkill, Peter H, Scanlan, David J, Kolowrat, Christian, Zubkov, MV, Zubkov, Mikhail V, Terry, MJ, Tarran, Glen A., Scanlan, David J., Scanlan, DJ, Burkill, Peter H., Tarran, GA, Terry, Matthew J., Burkill, PH, Zubkov, Mikhail V., Kolowrat, CK, Mary, I, Garczarek, LG   +20 more
core   +1 more source

Non-canonical amino acid labeling in proteomics and biotechnology

Journal of Biological Engineering, 2019
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries.
Aya M. Saleh, Kristen M. Wilding, Sarah Calve, Bradley C. Bundy, Tamara L. Kinzer-Ursem   +4 more
doaj   +1 more source

Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3 [PDF]

, 2004
Amino acids are regarded as the nitrogen ‘currency’ of plants. Amino acids can be taken up from the soil directly or synthesized from inorganic nitrogen, and then circulated in the plant via phloem and xylem.
Sakiko Okumoto, Leister, D., Frommer, Wolf B., Wolf B. Frommer, Okumoto, Sakiko, FISCHER, Wolf N, Stierhof, York Dieter, Frommer, W. B., Stierhof, Y., Stierhof, Y.-D., Fischer, W., Wolfgang Koch, Koch, W., FROMMER, Wolf B, Mechthild Tegeder, Fischer, Wolf N., Leister, Dario, Wolf N. Fischer, Koch, Wolfgang, Okumoto, S., Tegeder, Mechthild, Dario Leister, Tegeder, M., Biehl, A., York Dieter Stierhof, Alexander Biehl, Biehl, Alexander, Fischer, W. N., Frommer, W.   +28 more
core   +2 more sources

Identification of a novel C6 protein encoded by tomato leaf curl China virus

Phytopathology Research, 2022
Begomoviruses cause significant losses to a wide range of crops worldwide, and a great progress has been made in characterizing some noncanonical proteins encoded by begomoviruses.
Zhiyuan Wang, Yaqin Wang, Rosa Lozano-Duran, Tao Hu, Xueping Zhou   +4 more
doaj   +1 more source

In Vivo Incorporation of Multiple Unnatural Amino Acids [PDF]

, 2009
Unnatural amino acid (UAA) incorporation is an invaluable technique that is seeing increased use. THG73 is an amber suppressor tRNA used to incorporate > 100 residues at the UAG, amber stop codon, in Xenopus oocytes.
Rodriguez, Erik Ali
core   +1 more source