Forced Ambiguity of the Leucine Codons for Multiple-Site-Specific Incorporation of a Noncanonical Amino Acid. [PDF]
PLoS ONE, 2016 Multiple-site-specific incorporation of a noncanonical amino acid into a recombinant protein would be a very useful technique to generate multiple chemical handles for bioconjugation and multivalent binding sites for the enhanced interaction.
Inchan Kwon, Eun Sil Choi
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Advances in Noncanonical Amino Acid Incorporation for Enzyme Engineering Applications [PDF]
CHIMIA, 2023 Incorporation of noncanonical amino acids (ncAAs) via genetic code expansion (GCE) opens up new possibilities for chemical biology. The technology has led to the development of novel xenobiotic enzymes with tailored properties which can serve as entry ...
Sandro Giger, Rebecca Buller
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Engineering enzymes for noncanonical amino acid synthesis. [PDF]
Chem Soc Rev, 2018 The standard proteinogenic amino acids grant access to a myriad of chemistries that harmonize to create life. Outside of these twenty canonical protein building blocks are countless noncanonical amino acids (ncAAs), either found in nature or created by ...
Almhjell PJ, Boville CE, Arnold FH.
europepmc +4 more sources
Amino acid racemization dating: Method development using African ostrich (Struthio camelus) eggshell [PDF]
, 2013 Ostrich eggshell (OES) is ubiquitous in archaeological sites in Africa and is a favoured substrate for amino acid racemisation (AAR) geochronology, yielding consistent results and thought to approximate a closed system with respect to protein diagenesis.
Crisp, Molly Katherine
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Photoregulation of PRMT-1 Using a Photolabile Non-Canonical Amino Acid
Molecules, 2021 Protein methyltransferases are vital to the epigenetic modification of gene expression. Thus, obtaining a better understanding of and control over the regulation of these crucial proteins has significant implications for the study and treatment of ...
Elizabeth A. King +8 more
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A mutation in amino acid permease AAP6 reduces the amino acid content of the Arabidopsis sieve elements but leaves aphid herbivores unaffected [PDF]
, 2010 The aim of this study was to investigate the role of the amino acid permease gene AAP6 in regulating phloem amino acid composition and then to determine the effects of this altered diet on aphid performance.
Newbury, H. J. +6 more
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Insights into receptor structure and dynamics at the surface of living cells
Nature Communications, 2023 It is challenging to approach protein structures in living cells. Here the authors investigate Interleukin-4 receptor alpha, which has a noncanonical amino acid incorporated at different locations, and see that evaluating click efficiency with calibrated
Frederik Steiert +5 more
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Frontiers in Molecular Biosciences, 2022 Pyrrolysyl-tRNA synthetase (PylRS) is frequently used for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins. Recently, the active site of Methanomethylophilus alvus PylRS (MaPylRS) has been rationally engineered to expand its ...
Jonathan T. Fischer +3 more
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Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis. [PDF]
Biochim Biophys Acta Gen Subj, 2017 Gan Q, Fan C.
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A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency
Communications Biology, 2023 The nek4, eIK1, and eIK2 sensor kinases play a key role in Plasmodium amino acid sensing, enabling these parasites to fine-tune replication and development in response to amino acid availability.
Inês M. Marreiros +9 more
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