Current Research in BiotechnologySelective identification of translationally active cells remains a key challenge in linking microbial function to population dynamics. Bioorthogonal non-canonical amino acid tagging (BONCAT) enables pulse-labelling of newly synthesized proteins in live ...
Yumechris Amekan +5 more
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Visualizing conformational dynamics of proteins in solution and at the cell membrane
eLife, 2018 Conformational dynamics underlie enzyme function, yet are generally inaccessible via traditional structural approaches. FRET has the potential to measure conformational dynamics in vitro and in intact cells, but technical barriers have thus far limited ...
Sharona E Gordon +2 more
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Biophysics and Protein Engineering with Noncanonical Amino Acids [PDF]
, 2011 Noncanonical amino acids are tools for expanding and altering the chemical functionalities available within proteins. Much recent work has focused on developing biosynthetic means for incorporating noncanonical amino acids into proteins, and applications
Van Deventer, James A.
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A noncanonical amino acid-based relay system for site-specific protein labeling
, 2018 A noncanonical amino acid-based relay system spanning the biosynthesis, incorporation, and bioconjugation of p-aminophenylalanine was developed for site-specific protein labeling.
Juan Tang +6 more
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Effects of dietary long-chain polyunsaturated fatty acids on plasma amino acids and indices of protein metabolism in infants: Results from a randomized clinical trial [PDF]
, 1998 Background/Aim: Previous studies in vitro and in animals in vivo found that alpha-linolenic acid (C18:3 omega 3) may enhance oxidative damage of essential amino acids.
Burus, I. +2 more
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Replacing 32 Proline Residues by a Noncanonical Amino Acid Results in a Highly Active DNA Polymerase
, 2016 Protein engineering may be achieved by rational design, directed evolution-based methods, or computational protein design. Mostly these methods make recourse to the restricted pool of the 20 natural amino acids.
Bastian Holzberger (2253253) +1 more
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The catalytic subunit of the system L1 amino acid transporter (Slc7a5) facilitates nutrient signalling in mouse skeletal muscle [PDF]
, 2014 The System L1-type amino acid transporter mediates transport of large neutral amino acids (LNAA) in many mammalian cell-types. LNAA such as leucine are required for full activation of the mTOR-S6K signalling pathway promoting protein synthesis and cell ...
Adel F M Ibrahim +31 more
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Replacing 32 Proline Residues by a Noncanonical Amino Acid Results in a Highly Active DNA Polymerase
, 2010 Protein engineering may be achieved by rational design, directed evolution-based methods, or computational protein design. Mostly these methods make recourse to the restricted pool of the 20 natural amino acids.
Holzberger, Bastian +3 more
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Inhibitory postsynaptic actions of taurine, GABA and other amino acids on motoneurons of the isolated frog spinal cord [PDF]
, 1975 The actions of glycine, GABA, α-alanine, β-alanine and taurine were studied by intracellular recordings from lumbar motoneurons of the isolated spinal cord of the frog.
Krumnikl, J. +9 more
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Frontiers in Cellular and Infection Microbiology, 2018 Burkholderia pseudomallei and B. mallei are the causative agents of melioidosis and glanders, respectively, and are often fatal to humans and animals. Owing to the high fatality rate, potential for spread by aerosolization, and the lack of efficacious ...
Magdalena Franco +6 more
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