Results 41 to 50 of about 10,782 (215)

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source

Characterization of cereulide synthetase, a toxin-producing macromolecular machine.

PLoS ONE, 2015
Cereulide synthetase is a two-protein nonribosomal peptide synthetase system that produces a potent emetic toxin in virulent strains of Bacillus cereus.
Diego A Alonzo, Nathan A Magarvey, T Martin Schmeing   +2 more
doaj   +1 more source

Piecing together nonribosomal peptide synthesis

Current Opinion in Structural Biology, 2018
Nonribosomal peptide synthetases (NRPSs) produce peptide products with wide-ranging biological activities. NRPSs are macromolecular machines with modular assembly-line logic, a complex catalytic cycle, moving parts and multiple active sites. They are organized into repeating sets of domains, called modules.
Reimer, Janice M., Haque, Asfarul S., Tarry, Michael J., Schmeing, T. Martin   +3 more
openaire   +3 more sources

Mining microbial genomes for new natural products and biosynthetic pathways [PDF]

, 2008
Analyses of microbial genome sequences have revealed numerous examples of ‘cryptic’ or ‘orphan’ biosynthetic gene clusters, with the potential to direct the production of novel, structurally complex natural products.
G. L. Challis, Challis, Gregory L.
core   +1 more source

Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach

MicrobiologyOpen, 2016
Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans‐acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359.
Birgit Uytterhoeven, Kenny Appermans, Lijiang Song, Joleen Masschelein, Thomas Lathouwers, Chris W. Michiels, Rob Lavigne   +6 more
doaj   +1 more source

Dissecting and Exploiting Nonribosomal Peptide Synthetases

Acta Biochimica et Biophysica Sinica, 2004
A large number of therapeutically useful cyclic and linear peptides of bacteria or fungal origin are synthesized via a template-directed, nucleic-acid-independent nonribosomal mechanism. This process is carried out by mega-enzymes called nonribosomal peptide synthetases (NRPSs).
Qing-Tao, Shen, Xiu-Lan, Chen, Cai-Yun, Sun, Yu-Zhong, Zhang   +3 more
openaire   +2 more sources

Novel chemical probes for the investigation of nonribosomal peptide assembly [PDF]

, 2017
Chemical probes were devised and evaluated for the capture of biosynthetic intermediates involved in the bio-assembly of the nonribosomal peptide echinomycin. Putative intermediate peptide species were isolated and characterised, providing fresh insights
Manuela Tosin, Y. T. C. Ho, Bushell, Dexter P., Ho, Y. T. Candace, Ghiringhelli, Francesca, Havemann, Judith, O. Köstner, Wilkening, Ina, E. Riva, Daniel J. Leng, Elena Riva, Riva, Elena, Judith Havemann, Francesca Ghiringhelli, J. Havemann, Otto Köstner, Leng, Daniel J., Passarella, Daniele, Dexter P. Bushell, I. Wilkening, Y. T. Candace Ho, D. P. Bushell, D. Passarella, M. Tosin, Daniele Passarella, F. Ghiringhelli, Ina Wilkening, Tosin, Manuela, Köstner, Otto, D. J. Leng   +29 more
core   +1 more source

Sfp-type PPTase inactivation promotes bacterial biofilm formation and ability to enhance wheat drought tolerance

Frontiers in Microbiology, 2015
Paenibacillus polymyxa is a common soil bacterium with broad range of practical applications. An important group of secondary metabolites in P. polymyxa are nonribosomal peptide and polyketide derived metabolites (NRP/PK).
Salme eTimmusk
doaj   +1 more source

Antibacterial Property of a Coral-Associated Bacterium Pseudoalteromonas luteoviolacea Against Shrimp Pathogenic Vibrio harveyi (In Vitro Study)

Hayati Journal of Biosciences, 2005
A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the nonribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S ...
OCKY KARNA RADJASA, TORBEN MARTENS, HANS-PETER GROSSART, AGUS SABDONO, MEINHARD SIMON, TONNY BACHTIAR   +5 more
doaj   +3 more sources

Prediction of monomer isomery in Florine: a workflow dedicated to nonribosomal peptide discovery. [PDF]

PLoS ONE, 2014
Nonribosomal peptides represent a large variety of natural active compounds produced by microorganisms. Due to their specific biosynthesis pathway through large assembly lines called NonRibosomal Peptide Synthetases (NRPSs), they often display complex ...
Thibault Caradec, Maude Pupin, Aurélien Vanvlassenbroeck, Marie-Dominique Devignes, Malika Smaïl-Tabbone, Philippe Jacques, Valérie Leclère   +6 more
doaj   +1 more source