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ATPase and helicase activities of porcine epidemic diarrhea virus nsp13
Veterinary Microbiology, 2021 Porcine epidemic diarrhea virus (PEDV) is a reemerging Alphacoronavirus that causes lethal diarrhea in piglets. Coronavirus nonstructural protein 13 (nsp13) encodes helicase, which plays pivotal roles during viral replication by unwinding viral RNA. However, the biochemical characterization of PEDV nsp13 remains largely unknown.
Zhen Ding, Puxian Fang, Shaobo Xiao
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Molecular cloning, expression, and purification of SARS-CoV nsp13
Protein Expression and Purification, 2005 The SARS-nsp13 protein was identified as an mRNA cap1 methyltransferase. In this study, the nsp13 gene was cloned from the SARS-CoV PUMC02 strain viral RNA by RT-PCR, and inserted into the expression plasmid pET30a(+). The recombinant plasmid pET30a(+)-nsp13 was confirmed by restriction enzymes and sequencing analysis, and transformed into Escherichia ...
Jiangang Yuan, Boqin Qiang
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The stalk domain of SARS-CoV-2 NSP13 is essential for its helicase activity
Biochemical and Biophysical Research Communications, 2022 COVID-19, caused by SARS-CoV-2, has been spreading worldwide for more than two years and has led to immense challenges to human health. Despite the great efforts that have been made, our understanding of SARS-CoV-2 is still limited. The viral helicase, NSP13 is an important enzyme involved in SARS-CoV-2 replication and transcription.
Kun Yue, Bin Yao, Zhaohui Qian
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Diketo acid inhibitors of nsp13 of SARS-CoV-2 block viral replication
Antiviral Research, 2023 For RNA viruses, RNA helicases have long been recognized to play critical roles during virus replication cycles, facilitating proper folding and replication of viral RNAs, therefore representing an ideal target for drug discovery. SARS-CoV-2 helicase, the non-structural protein 13 (nsp13) is a highly conserved protein among all known coronaviruses, and,
Angela Corona +2 more
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Antiviral Research, 2022 The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) helicase NSP13 plays a conserved role in the replication of coronaviruses and has been identified as an ideal target for the development of antiviral drugs against SARS-CoV-2. Here, we identify a novel NSP13 helicase inhibitor punicalagin (PUG) through high-throughput screening.
Yanqun Wang, Zhonghui Lin
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ACS Infectious Diseases, 2022
SARS-CoV-2 non-structural protein 13 (nsp13) is a highly conserved helicase and RNA 5'-triphosphatase. It uses the energy derived from the hydrolysis of nucleoside triphosphates for directional movement along the nucleic acids and promotes the unwinding of double-stranded nucleic acids.
Aliakbar Khalili Yazdi +2 more
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SARS-CoV-2 non-structural protein 13 (nsp13) is a highly conserved helicase and RNA 5'-triphosphatase. It uses the energy derived from the hydrolysis of nucleoside triphosphates for directional movement along the nucleic acids and promotes the unwinding of double-stranded nucleic acids.
Aliakbar Khalili Yazdi +2 more
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SARS‐CoV‐2 NSP13 interacts with host IRF3, blocking antiviral immune responses
Journal of Medical Virology, 2023AbstractCoronavirus disease 2019 (COVID‐19), caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), poses an unprecedented threat to human health since late 2019. Notably, the progression of the disease is associated with impaired antiviral interferon (IFN) responses.
Kuan Feng, Yuan-Qin Min, Fei Deng
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Natural Compounds Inhibit SARS-CoV-2 nsp13 Unwinding and ATPase Enzyme Activities
ACS Pharmacology and Translational Science, 2022 SARS-CoV-2 infection is still spreading worldwide, and new antiviral therapies are an urgent need to complement the approved vaccine preparations. SARS-CoV-2 nps13 helicase is a validated drug target participating in the viral replication complex and possessing two associated activities: RNA unwinding and 5'-triphosphatase.
Angela Corona +2 more
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Characterization of the SARS-CoV-2 Nsp13 Helicase
2023Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent responsible for the coronavirus disease of 2019 (COVID-19) pandemic, which has infected millions of people worldwide. To date, several vaccines and antivirals have been developed against SARS-CoV-2; however, its tendency to mutate rapidly poses a continued threat to ...
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[Prokaryotic expression and helicase activity analysis of PDCoV NSP13].
Sheng wu gong cheng xue bao = Chinese journal of biotechnologyPorcine deltacoronavirus (PDCoV) is a major pathogen causing fatal diarrhea in suckling piglets, and there is currently a lack of effective vaccines and drugs to prevent and control the virus. The nonstructural protein 13 (NSP13) serves as a virus-coded helicase and is considered to be a crucial target for antiviral drugs, making it imperative to ...
Lihan, Tao +4 more
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