Results 71 to 80 of about 7,712 (169)

Characterization of rotavirus NSP2/NSP5 interactions and the dynamics of viroplasm formation

Journal of General Virology, 2004
Viroplasms are discrete structures formed in the cytoplasm of rotavirus-infected cells and constitute the replication machinery of the virus. The non-structural proteins NSP2 and NSP5 localize in viroplasms together with other viral proteins, including the polymerase VP1, VP3 and the main inner-core protein, VP2. NSP2 and NSP5 interact with each other,
Catherine, Eichwald, José Francisco, Rodriguez, Oscar R, Burrone   +2 more
openaire   +2 more sources

Prediction of promiscuous epitopes in NSP2 of Chikungunya virus: An in-silico approach

Tropical Biomedicine, 2020
This study is focused towards developing a global consensus sequence of nonstructural protein 2 (NSP2), a protease of Chikungunya Virus (CHIKV) and predict immunogenic promiscuous T-cell epitopes based on various bioinformatics tools. To date, no epitope data is available for the Chikungunya virus in the IEDB database.
F, Fazal, T, Anwar, Y, Waheed, F, Parvaiz   +3 more
openaire   +2 more sources

Unraveling the Potential of MXenes in Electrochemical Sensing: Mechanistic Insight, Design Principles, and Analytical Applications

Small Science, Volume 6, Issue 5, May 2026.
This review comprehensively examines recent progress in MXene‐based electrochemical sensors for the detection of glucose, hydrogen peroxide, heavy metals, pesticides, nitrites, phenolic pollutants, neurotransmitters, biomarkers, and pharmaceutical compounds.
Hichem Moulahoum, Faezeh Ghorbanizamani, Tomáš Kovářík, Kalim Deshmukh   +3 more
wiley   +1 more source

Identification of Inhibitors of Chikungunya virus nsP2 ATPase

Abstract Non-structural protein 2 (nsP2), which plays an essential role in replication of CHIKV, contains a protease, helicase, and methyltransferase-like domain. We executed a simple a screen using malachite green to detect compounds that decreased ATP hydrolysis and tested a library of diverse compounds to find inhibitors of CHIKV ...
Hernan Navarro, John E. Scott, Ginger R. Smith, Pegah Ghiabi, Elisa Gibson, Peter Loppnau, Rachel J. Harding, Mohammad Anwar Hossain, Muthu Ramalingam Bose, Kenneth H. Pearce, Eric M. Merten, Timothy M. Willson, Peter J. Brown   +12 more
openaire   +2 more sources

Identification of the active site residues in the nsP2 proteinase of sindbis virus

Virology, 1992
The nonstructural polyproteins of Sindbis virus are processed by a virus-encoded proteinase which is located in the C-terminal domain of nsP2. Here we have performed a mutagenic analysis to identify the active site residues of this proteinase. Substitution of other amino acids for either Cys-481 or His-558 completely abolished proteolytic processing of
Strauss, Ellen G., de Groot, Raoul J., Levinson, Randy, Strauss, James H.   +3 more
openaire   +3 more sources

Alphaviral Capsid Proteins Inhibit Stress Granule Assembly via Competitive RNA Binding With G3BP1

Advanced Science, Volume 13, Issue 20, 9 April 2026.
Stress granules exert antiviral functions. This study illustrates a conserved function of alphaviral capsid proteins in modulating stress granules. Oligomerization mediated by a helical motif coupled with a positively charged intrinsically disordered region (IDR) directly competes with G3BP1 for RNA binding, thereby disrupting G3BP1‐RNA liquid–liquid ...
Yun Zhang, Yi Liu, Zhiying Yao, Haolong Lai, Xiaoxin Chen, Ziqiu Wang, Yiqiong Bao, Tingting Li, Xiaoming Zhou, Xiabin Chen, Peiguo Yang   +10 more
wiley   +1 more source

Identification of Direct-acting nsP2 Helicase Inhibitors with Antialphaviral Activity

Journal of Medicinal Chemistry
Abstract Alphaviruses are mosquito-borne RNA viruses that pose a significant public health threat, with no FDA-approved antiviral therapeutics available. The non-structural protein 2 helicase (nsP2hel) is an enzyme involved in unwinding dsRNA essential for alphavirus replication.
Muthu Ramalingam Bose, John D. Sears, Kacey M. Talbot, Yuan-Wei Norman Su, Scott Houliston, Mohammed Anwar Hossain, Zachary W. Davis-Gilbert, Chunqing Zhao, Hans J. Oh, Peter J. Brown, Marcia K. Sanders, Stella R. Moorman, Durbadal Ojha, Jane E. Burdick, Isabella Law, Noah L. Morales, Sabian A. Martinez, Peter Loppnau, Julia Garcia Perez, Adam M. Drobish, Thomas E. Morrison, Zachary J. Streblow, Daniel N. Streblow, Cheryl H. Arrowsmith, Ava Vargason, Rafael M. Couñago, Levon Halabelian, Jamie J. Arnold, Craig E. Cameron, Nathaniel J. Moorman, Mark T. Heise, Timothy M. Willson   +31 more
openaire   +2 more sources

Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

Virology Journal, 2012
Background The regions in the middle of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) have been shown to be nonessential for PRRSV replication, and these nonessential regions are different in various viral ...
Xu Yan-Zhao, Zhou Yan-Jun, Zhang Shan-Rui, Tong Wu, Li Ling, Jiang Yi-Feng, Tong Guang-Zhi   +6 more
doaj   +1 more source

Coronavirus Nsp3 Hijacks CLTC to Modulate Autophagosome Nucleation for Promoting DMV Formation and Viral Replication

Advanced Science, Volume 13, Issue 24, 27 April 2026.
In wild‐type cells, FIPV infection recruits CLTC to nsp3, facilitates DMV biogenesis and block autophagic flux to promote viral replication. CLTC knockout impairs autophagosome nucleation by reducing Beclin1–ATG14 complex expression. This disrupts the formation of autophagic precursor membranes, thereby preventing their hijacking by nsp3 for DMV ...
Juan Xu, Hang Li, Peng Liu, Zhe Jiao, Ding Zhang, Zhelin Su, Sai Niu, Jintao Zhang, Yuejun Shi, Guiqing Peng   +9 more
wiley   +1 more source

The Open Reading Frame 7b of the SARS‐CoV‐2 Disperse Trans‐Golgi and Activate the NLRP3 Inflammasome

Journal of Medical Virology, Volume 98, Issue 4, April 2026.
ABSTRACT Inflammasomes orchestrate the inflammatory response against bacterial and viral infections, thereby initiating the synthesis of pro‐inflammatory cytokines, mainly IL‐1β and IL‐18. SARS‐CoV‐2 infection induces an inflammatory response mediated by the activation of NLRP1 and NLRP3 inflammasomes.
Julio García‐Villalba, Laura Hurtado‐Navarro, Diego Angosto‐Bazarra, Alberto Baroja‐Mazo, Pablo Pelegrin   +4 more
wiley   +1 more source