Results 1 to 10 of about 9,413 (173)

Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation. [PDF]

PLoS ONE, 2016
Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous ...
Matthieu Gratia, Patrice Vende, Annie Charpilienne, Hilma Carolina Baron, Cécile Laroche, Emeline Sarot, Stéphane Pyronnet, Mariela Duarte, Didier Poncet   +8 more
doaj   +7 more sources

Cryo-EM reveals a double oligomeric ring scaffold of the CHIKV nsP3 peptide in complex with the NTF2L domain of host G3BP1 [PDF]

mBio
Chikungunya virus (CHIKV) poses a severe threat to global public health. The interaction between CHIKV nsP3 and host G3BP1 is critical for viral replication. However, the exact structural mechanism of the nsP3–G3BP1 interaction is scarce. Here, we report
Yuanzhi Liu, Jie Wang, Yinze Han, Xiaoyan Xia, Rui Zeng, Xinyu Fan, Bo Zhang, Kaituo Wang, Jian Lei   +8 more
doaj   +3 more sources

Identification of NSP3 (SH2D3C) as a Prognostic Biomarker of Tumor Progression and Immune Evasion for Lung Cancer and Evaluation of Organosulfur Compounds from Allium sativum L. as Therapeutic Candidates [PDF]

Biomedicines, 2021
The multi-domain non-structural protein 3 (NSP3) is an oncogenic molecule that has been concomitantly implicated in the progression of coronavirus infection. However, its oncological role in lung cancer and whether it plays a role in modulating the tumor
Yuan-Chieh Yeh, Bashir Lawal, Michael Hsiao, Tse-Hung Huang, Chi-Ying F. Huang   +4 more
doaj   +2 more sources

Mutation of CD2AP and SH3KBP1 Binding Motif in Alphavirus nsP3 Hypervariable Domain Results in Attenuated Virus [PDF]

Viruses, 2018
Infection by Chikungunya virus (CHIKV) of the Old World alphaviruses (family Togaviridae) in humans can cause arthritis and arthralgia. The virus encodes four non-structural proteins (nsP) (nsP1, nsp2, nsP3 and nsP4) that act as subunits of the virus ...
Margit Mutso, Ainhoa Moliner Morro, Cecilia Smedberg, Sergo Kasvandik, Muriel Aquilimeba, Mona Teppor, Liisi Tarve, Aleksei Lulla, Valeria Lulla, Sirle Saul, Bastian Thaa, Gerald M McInerney, Andres Merits, Margus Varjak   +13 more
doaj   +2 more sources

A structural roadmap for the formation of the coronavirus nsp3/nsp4 double membrane vesicle pore and its implications for polyprotein processing and replication/transcription [PDF]

Journal of Virology
Coronavirus replication is understood to occur within double membrane vesicles (DMVs) that arise during viral infection. Prior work has determined that these DMVs have characteristic pores formed from the non-structural viral proteins nsp3 and nsp4 ...
Jason K. Perry, Samuel Itskanov, John P. Bilello, Eric B. Lansdon   +3 more
doaj   +2 more sources

Structural and functional analyses of SARS-CoV-2 Nsp3 and its specific interactions with the 5’ UTR of the viral genome [PDF]

Microbiology Spectrum
Non-structural protein 3 (Nsp3) is the largest open reading frame encoded in the SARS-CoV-2 genome, essential for the formation of double-membrane vesicles (DMV) wherein viral RNA replication occurs.
Sofia Lemak, Tatiana Skarina, Deepak T. Patel, Peter J. Stogios, Alexei Savchenko   +4 more
doaj   +2 more sources

SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle [PDF]

Nature Communications, 2023
Coronavirus replication is associated with the remodeling of cellular membranes, resulting in the formation of double-membrane vesicles (DMVs). A DMV-spanning pore was identified as a putative portal for viral RNA.
Liv Zimmermann, Xiaohan Zhao, Jana Makroczyova, Moritz Wachsmuth-Melm, Vibhu Prasad, Zach Hensel, Ralf Bartenschlager, Petr Chlanda   +7 more
doaj   +2 more sources

Pyrimidone inhibitors targeting Chikungunya Virus nsP3 macrodomain by fragment-based drug design.

PLoS ONE, 2021
The macrodomain of nsP3 (nsP3MD) is highly conserved among the alphaviruses and ADP-ribosylhydrolase activity of Chikungunya Virus (CHIKV) nsP3MD is critical for CHIKV viral replication and virulence.
Sixue Zhang, Atefeh Garzan, Nicole Haese, Robert Bostwick, Yohanka Martinez-Gzegozewska, Lynn Rasmussen, Daniel N Streblow, Mark T Haise, Ashish K Pathak, Corinne E Augelli-Szafran, Mousheng Wu   +10 more
doaj   +3 more sources

In-silico prediction of novel drug-target complex of nsp3 of CHIKV through molecular dynamic simulation

Heliyon, 2020
Literature reported that nsp3 of CHIKV is an important target for the designing of drug as it involves in the replication, survival etc. Herein, about eighteen million molecules available in the ZINC database are filtered against nsp3 using RASPD.
Durgesh Kumar, Mahendra Kumar Meena, Kamlesh Kumari, Rajan Patel, Abhilash Jayaraj, Prashant Singh   +5 more
doaj   +2 more sources

Comparative Interactome Profiling of Nonstructural Protein 3 Across SARS-CoV-2 Variants Emerged During the COVID-19 Pandemic [PDF]

Viruses
SARS-CoV-2 virus and its variants remain a global health threat, due to their capacity for rapid evolution. Variants throughout the COVID-19 pandemic exhibited variations in virulence, impacting vaccine protection and disease severity.
Valeria Garcia Lopez, Lars Plate
doaj   +2 more sources