Pyrimidone inhibitors targeting Chikungunya Virus nsP3 macrodomain by fragment-based drug design.
PLoS ONE, 2021 The macrodomain of nsP3 (nsP3MD) is highly conserved among the alphaviruses and ADP-ribosylhydrolase activity of Chikungunya Virus (CHIKV) nsP3MD is critical for CHIKV viral replication and virulence.
Sixue Zhang +10 more
doaj +5 more sources
The Chikungunya Virus nsP3 Macro Domain Inhibits Activation of the NF-κB Pathway. [PDF]
Viruses, 2019 AbstractThe role of the chikungunya virus (CHIKV) non-structural protein 3 (nsP3) in the virus lifecycle is poorly understood. The protein comprises 3 domains. The N-terminus is a macro domain, biochemically characterised to bind both RNA and ADP-ribose, and to possess ADP-ribosyl hydrolase activity – an enzymatic activity that removes ADP-ribose from ...
Roberts GC, Stonehouse NJ, Harris M.
europepmc +8 more sources
Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation. [PDF]
PLoS ONE, 2016 Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous ...
Matthieu Gratia +8 more
doaj +6 more sources
Interactions of Alphavirus nsP3 Protein with Host Proteins [PDF]
Frontiers in Microbiology, 2018 Alphaviruses are members of the Togaviridae family and are grouped into two categories: arthritogenic and encephalitic. Arthritogenic alphavirus infections, as the name implies, are associated with arthritic outcomes while encephalitic alphavirus ...
Tyler Lark +2 more
doaj +3 more sources
Discovery and Development Strategies for SARS-CoV-2 NSP3 Macrodomain Inhibitors
Pathogens, 2023 The worldwide public health and socioeconomic consequences caused by the COVID-19 pandemic highlight the importance of increasing preparedness for viral disease outbreaks by providing rapid disease prevention and treatment strategies.
Marion Schuller +8 more
doaj +4 more sources
Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles [PDF]
mBio, 2013 Coronaviruses (CoV), like other positive-stranded RNA viruses, redirect and rearrange host cell membranes for use as part of the viral genome replication and transcription machinery.
Megan M. Angelini +3 more
doaj +2 more sources
Involvement of PRRSV NSP3 and NSP5 in the autophagy process
Virology Journal, 2019 Background Autophagy is an essential process in eukaryotic cells in which autophagosomes form to deliver cellular organelles and long-lived proteins to lysosomes for degradation. Many studies have recently identified the regulatory mechanisms involved in
Wei Zhang +4 more
doaj +3 more sources
Comparative Interactome Profiling of Nonstructural Protein 3 Across SARS-CoV-2 Variants Emerged During the COVID-19 Pandemic [PDF]
VirusesSARS-CoV-2 virus and its variants remain a global health threat, due to their capacity for rapid evolution. Variants throughout the COVID-19 pandemic exhibited variations in virulence, impacting vaccine protection and disease severity.
Valeria Garcia Lopez, Lars Plate
doaj +2 more sources
Cryo-EM reveals a double oligomeric ring scaffold of the CHIKV nsP3 peptide in complex with the NTF2L domain of host G3BP1 [PDF]
mBioChikungunya virus (CHIKV) poses a severe threat to global public health. The interaction between CHIKV nsP3 and host G3BP1 is critical for viral replication. However, the exact structural mechanism of the nsP3–G3BP1 interaction is scarce. Here, we report
Yuanzhi Liu +8 more
doaj +2 more sources
EMBO ReportsViruses interact with numerous host factors to facilitate viral replication and to dampen antiviral defense mechanisms. We currently have a limited mechanistic understanding of how SARS-CoV-2 binds host factors and the functional role of these ...
Dimitriya H Garvanska +23 more
doaj +2 more sources