Results 31 to 40 of about 14,750 (231)

The Enigmatic Alphavirus Non-Structural Protein 3 (nsP3) Revealing Its Secrets at Last [PDF]

Viruses, 2018
Alphaviruses encode 4 non-structural proteins (nsPs), most of which have well-understood functions in capping and membrane association (nsP1), polyprotein processing and RNA helicase activity (nsP2) and as RNA-dependent RNA polymerase (nsP4). The function of nsP3 has been more difficult to pin down and it has long been referred to as the more enigmatic
Benjamin Götte, Lifeng Liu, Gerald M. McInerney   +2 more
openalex   +5 more sources

SH3 domain-mediated recruitment of host cell amphiphysins by alphavirus nsP3 promotes viral RNA replication. [PDF]

PLoS Pathogens, 2011
Among the four non-structural proteins of alphaviruses the function of nsP3 is the least well understood. NsP3 is a component of the viral replication complex, and composed of a conserved aminoterminal macro domain implicated in viral RNA synthesis, and ...
Maarit Neuvonen, Arunas Kazlauskas, Miika Martikainen, Ari Hinkkanen, Tero Ahola, Kalle Saksela   +5 more
doaj   +1 more source

Antagonism of mosquito innate immunity by the chikungunya virus nsP3 protein

Access Microbiology, 2019
Chikungunya virus (CHIKV) is a re-emerging Alphavirus transmitted by Aedes mosquitos and causing fever, rash and chronic arthralgia. There are no vaccines or antiviral agents available for CHIKV therefore it is important to understand the molecular details of virus replication.
Jinchao Guo, Yanni Gao, Mark Harris
openalex   +2 more sources

Design, Synthesis and Evaluation of Inhibitors of the SARS-CoV2 nsp3 Macrodomain [PDF]

Bioorganic & Medicinal Chemistry, 2022
AbstractA series of amino acid based7H-pyrrolo[2,3-d]pyrimidines were designed and synthesized to discern the structure activity relationships against the SARS-CoV-2 nsp3 macrodomain (Mac1), an ADP-ribosylhydrolase that is critical for coronavirus replication and pathogenesis.
Lavinia M. Sherrill, Elva E. Joya, AnnMarie Walker, Anuradha Roy, Yousef M. Alhammad, Moriama Atobatele, Sarah Wazir, George Abbas, Patrick Keane, Junlin Zhuo, Anthony K.L. Leung, David K. Johnson, Lari Lehtiö, Anthony R. Fehr, Dana Ferraris   +14 more
openaire   +3 more sources

Sequestration of G3BP coupled with efficient translation inhibits stress granules in Semliki Forest virus infection [PDF]

, 2012
Dynamic, mRNA-containing stress granules (SGs) form in the cytoplasm of cells under environmental stresses, including viral infection. Many viruses appear to employ mechanisms to disrupt the formation of SGs on their mRNAs, suggesting that they represent
Eng, Kai Er, Karlsson Hedestam, Gunilla B., Lulla, Aleksei, McInerney, Gerald M., Merits, Andres, Panas, Marc D., Varjak, Margus   +6 more
core   +4 more sources

Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes

IUCrJ, 2020
Among 15 nonstructural proteins (Nsps), the newly emerging Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) encodes a large, multidomain Nsp3. One of its units is the ADP-ribose phosphatase domain (ADRP; also known as the macrodomain, MacroD),
Karolina Michalska, Youngchang Kim, Robert Jedrzejczak, Natalia I. Maltseva, Lucy Stols, Michael Endres, Andrzej Joachimiak   +6 more
doaj   +1 more source

"Fertile" Mutations in SARS-CoV-2 RNA More Frequently Occurred in Hairpin Loops That Determine Virus Evolution. [PDF]

APMIS
ABSTRACT RNA hairpins may constitute a foundation of genetic evolution both in viruses and other organisms. Stem‐loops theoretically comprise a stable part, the double‐stranded stem, and a single‐stranded loop allowing evolution. Here we tested for SARS‐CoV‐2 if “fertile” mutations were in loops while mutations in stems were poorly tolerated and rarely
Colson P, Pontarotti P, Fantini J, Levasseur A, Devaux C, Raoult D.   +5 more
europepmc   +2 more sources

SARS-CoV-2 nucleocapsid and Nsp3 binding: an in silico study [PDF]

Archives of Microbiology, 2020
Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The virus contains a large genome that encodes four structural proteins, small envelope (E), matrix (M), nucleocapsid phosphoprotein (N), spike (S), and 16 nonstructural proteins (nsp1-16) that together, ensure replication of the virus in ...
Khan, Muhammad Tahir, Zeb, Muhammad Tariq, Ahsan, Hina, Ahmed, Abrar, Ali, Arif, Akhtar, Khalid, Malik, Shaukat Iqbal, Cui, Zhilei, Ali, Sajid, Khan, Anwar Sheed, Ahmad, Manzoor, Wei, Dong-Qing, Irfan, Muhammad   +12 more
openaire   +2 more sources

Formation of nsP3-Specific Protein Complexes during Sindbis Virus Replication [PDF]

Journal of Virology, 2006
ABSTRACTAlphaviruses are arthropod-borne viruses (arboviruses) that include a number of important human and animal pathogens. Their replication proceeds in the cytoplasm of infected cells and does not directly depend on nuclei. Alphaviruses encode only four nonstructural proteins that are required for the replication of viral genome and transcription ...
Elena, Frolova, Rodion, Gorchakov, Natalia, Garmashova, Svetlana, Atasheva, Leoncio A, Vergara, Ilya, Frolov   +5 more
openaire   +2 more sources

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies.

PLoS ONE, 2021
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize.
Lee A Armstrong, Sven M Lange, Virginia Dee Cesare, Stephen P Matthews, Raja Sekhar Nirujogi, Isobel Cole, Anthony Hope, Fraser Cunningham, Rachel Toth, Rukmini Mukherjee, Denisa Bojkova, Franz Gruber, David Gray, Paul G Wyatt, Jindrich Cinatl, Ivan Dikic, Paul Davies, Yogesh Kulathu   +17 more
doaj   +1 more source