Results 51 to 60 of about 9,413 (173)

Structure-Based High-Throughput Virtual Screening and Molecular Dynamics Simulation for the Discovery of Novel SARS-CoV-2 NSP3 Mac1 Domain Inhibitors

Viruses, 2023
Since the emergence of SARS-CoV-2, many genetic variations within its genome have been identified, but only a few mutations have been found in nonstructural proteins (NSPs).
Behnaz Yazdani, Hajar Sirous, Simone Brogi, Vincenzo Calderone   +3 more
doaj   +1 more source

Formation of nsP3-Specific Protein Complexes during Sindbis Virus Replication [PDF]

Journal of Virology, 2006
ABSTRACTAlphaviruses are arthropod-borne viruses (arboviruses) that include a number of important human and animal pathogens. Their replication proceeds in the cytoplasm of infected cells and does not directly depend on nuclei. Alphaviruses encode only four nonstructural proteins that are required for the replication of viral genome and transcription ...
Elena, Frolova, Rodion, Gorchakov, Natalia, Garmashova, Svetlana, Atasheva, Leoncio A, Vergara, Ilya, Frolov   +5 more
openaire   +2 more sources

Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein

Antiviral Research, 2018
The multi-domain non-structural protein 3 (Nsp3) is the largest protein encoded by the coronavirus (CoV) genome, with an average molecular mass of about 200 kD. Nsp3 is an essential component of the replication/transcription complex. It comprises various domains, the organization of which differs between CoV genera, due to duplication or absence of ...
Lei, Jian, Kusov, Yuri, Hilgenfeld, Rolf
openaire   +2 more sources

Nsp3-N interactions are critical for SARS-CoV-2 fitness and virulence

Proceedings of the National Academy of Sciences, 2023
SARS-CoV-2, the causative agent of COVID-19 encodes at least 16 nonstructural proteins of variably understood function. Nsp3, the largest nonstructural protein contains several domains, including a SARS-unique domain (SUD), which occurs only in Sarbecovirus .
Pengfei Li, Biyun Xue, Nicholas J. Schnicker, Lok-Yin Roy Wong, David K. Meyerholz, Stanley Perlman   +5 more
openaire   +2 more sources

Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing

Viruses, 2018
Positive-strand RNA viruses, such as coronaviruses, induce cellular membrane rearrangements during replication to form replication organelles allowing for efficient viral RNA synthesis.
Nicole Doyle, Benjamin W. Neuman, Jennifer Simpson, Philippa C. Hawes, Judith Mantell, Paul Verkade, Hasan Alrashedi, Helena J. Maier   +7 more
doaj   +1 more source

Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3

Viruses, 2021
Venezuelan equine encephalitis virus (VEEV) is a new world alphavirus and a category B select agent. Currently, no FDA-approved vaccines or therapeutics are available to treat VEEV exposure and resultant disease manifestations. The C-terminus of the VEEV
Allison Bakovic, Nishank Bhalla, Farhang Alem, Catherine Campbell, Weidong Zhou, Aarthi Narayanan   +5 more
doaj   +1 more source

Novel Functions of the Alphavirus Nonstructural Protein nsP3 C-Terminal Region [PDF]

Journal of Virology, 2010
ABSTRACT The functions of the alphavirus-encoded nonstructural protein nsP3 during infection are poorly understood. In contrast, nsP1, nsP2, and nsP4 have known enzymatic activities and functions. A functional analysis of the C-terminal region of nsP3 of Semliki Forest virus revealed the presence of a degradation signal that ...
Margus, Varjak, Eva, Zusinaite, Andres, Merits   +2 more
openaire   +2 more sources

Genome-wide analysis of protein–protein interactions and involvement of viral proteins in SARS-CoV-2 replication

Cell & Bioscience, 2021
Background Analysis of viral protein–protein interactions is an essential step to uncover the viral protein functions and the molecular mechanism for the assembly of a viral protein complex.
Yiling Jiang, Kuijie Tong, Roubin Yao, Yuanze Zhou, Hanwen Lin, Liubing Du, Yunyun Jin, Liu Cao, Jingquan Tan, Xing-Ding Zhang, Deyin Guo, Ji-An Pan, Xiaoxue Peng   +12 more
doaj   +1 more source

SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species

Emerging Microbes and Infections, 2021
The genome of SARS-CoV-2 encodes two viral proteases (NSP3/papain-like protease and NSP5/3C-like protease) that are responsible for cleaving viral polyproteins during replication.
Mehdi Moustaqil, Emma Ollivier, Hsin-Ping Chiu, Sarah Van Tol, Paulina Rudolffi-Soto, Christian Stevens, Akshay Bhumkar, Dominic J. B. Hunter, Alexander N. Freiberg, David Jacques, Benhur Lee, Emma Sierecki, Yann Gambin   +12 more
doaj   +1 more source

Novel NMR Assignment Strategy Reveals Structural Heterogeneity in Solution of the nsP3 HVD Domain of Venezuelan Equine Encephalitis Virus

Molecules, 2020
In recent years, intrinsically disordered proteins (IDPs) and disordered domains have attracted great attention. Many of them contain linear motifs that mediate interactions with other factors during formation of multicomponent protein complexes.
Peter Agback, Andrey Shernyukov, Francisco Dominguez, Tatiana Agback, Elena I. Frolova   +4 more
doaj   +1 more source