Results 61 to 70 of about 12,322 (192)

The importance of the NSP3 eIF4G- and RNA-binding domains.

, 2016
A: Expression of wild type or mutated NSP3. Lysate from BSRT7 cells transfected for 24 h with the expression vectors encoding wild type NSP3 (wt); NSP3 mutated at the RNA-binding domain (4G-BD) or the eIF4G-binding domain (RN-BD) alone or in combination;
Cécile Laroche (842228), Didier Poncet (371932), Mariela Duarte (842231), Hilma Carolina Baron (842227), Matthieu Gratia (842225), Stéphane Pyronnet (842230), Patrice Vende (842226), Annie Charpilienne (371930), Emeline Sarot (842229)   +8 more
core   +1 more source

Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation. [PDF]

PLoS ONE, 2016
Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous ...
Matthieu Gratia, Patrice Vende, Annie Charpilienne, Hilma Carolina Baron, Cécile Laroche, Emeline Sarot, Stéphane Pyronnet, Mariela Duarte, Didier Poncet   +8 more
doaj   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

Molecular modelling and structure-activity relationship of a natural derivative of o-hydroxybenzoate as a potent inhibitor of dual NSP3 and NSP12 of SARS-CoV-2: in silico study

Journal of Biomolecular Structure and Dynamics, 2022
The nsp3 macrodomain and nsp12 (RdRp) enzymes are strongly implicated in the virulent regulation of the host immune response and viral replication of SARS-CoV-2, making them plausible therapeutic targets for mitigating infectivity. Remdesivir remains the
Y. Ayipo, I. Ahmad, Y. Najib, Sikirat Kehinde Sheu, H. Patel, M. Mordi   +5 more
semanticscholar   +1 more source

Vaccination Reduces Shedding of Salmonid Alphavirus Subtype 3, but Bacterial Co‐Infection Influences the Effect

Journal of Fish Diseases, EarlyView.
ABSTRACT Waterborne horizontal transmission of viral diseases in fish relies on the release of infectious virus particles (termed shedding) into the aquatic environment. Both the rate and duration of shedding are critical for efficient viral spread, making interventions that reduce shedding valuable for disease control.
Søren Grove, H. Craig Morton, Dhamotharan Kannimuthu, HyeongJin Roh, Ravikumar M. Chovatia, Ma Michelle Peñaranda, Dawit Ghebretnsae, Kai Ove Skaftnesmo   +7 more
wiley   +1 more source

Proximity interactome of alphavirus replicase component nsP3 includes proviral host factors eIF4G and AHNAK

PLoS Pathogens
All positive-strand RNA viruses replicate their genomes in association with modified intracellular membranes, inducing either membrane invaginations termed spherules, or double-membrane vesicles. Alphaviruses encode four non-structural proteins nsP1-nsP4,
Aditya Thiruvaiyaru, Sari Mattila, M. Sadeghi, Krystyna Naumenko, A. Merits, M. Varjosalo, T. Ahola   +6 more
semanticscholar   +1 more source

Functions of Alphavirus Macrodomain-containing protein nsP3 [PDF]

, 2011
Alphaviruses are positive strand RNA viruses that replicate in association with cellular membranes. The viral RNA replication complex consists of four non-structural proteins nsP1-nsP4 which are essential for viral replication.
Neuvonen, Maarit
core  

Expression of nsP3 in C2C12 cells.

, 2019
Cells were transfected with wildtype and the indicated mutant pcDNA3.1-nsP3 plasmids and cell lysates harvested at 48 h.p.t. into GLB. Lysates were analysed for expression of nsP3 by western blot.
Yanni Gao (6250973), Joseph Ward (645977), Niluka Goonawardane (4782675), Andrew Tuplin (3163998), Mark Harris (33805)   +4 more
core   +1 more source

Substrate‐Selective Inhibition of the SARS‐CoV‐2 Papain‐Like Protease: Inhibition of Hydrolysis of Human Over Viral Substrates

Chemistry – A European Journal, Volume 32, Issue 36, 24 September 2026.
The SARS‐CoV‐2 papain‐like protease (PLpro) is a medicinal chemistry target. Here we report mass spectrometry assays employing oligopeptide substrates based on the sequences of the viral polyproteins 1a/1ab and on an ISG15‐modified human protein, which enabled the identification of substrate‐selective PLpro inhibitors.
Sakshi Sharma, Peter A. C. Wing, Wojtek Treyde, Shyam Basak, Simeon D. Draganov, Taylah Andrews‐Clark, Eidarus Salah, Petra Lukacik, Claire Strain‐Damerell, Adán Pinto‐Fernández, Martin A. Walsh, Fernanda Duarte, Christopher J. Schofield, Lennart Brewitz   +13 more
wiley   +1 more source

Determination of MHV-nsp3 pausing site.

, 2016
(A) Nsp3 is the largest protein encoded in the MHV replicase gene and contains two ubiquitin-related domains (green), a hypervariable acidic domain (red), two papain-like cysteine proteinase domains (PLP1 and PLP2; blue), a poly (ADP-ribose) binding ...
Ian Brierley (8691), Joshua D. Jones (2502994), Betty Y.-W. Chung (486028), Stuart G. Siddell (330311), Nerea Irigoyen (2502991), Andrew E. Firth (122998)   +5 more
core   +1 more source