Results 71 to 80 of about 519,246 (298)

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Dynamic subcellular localization of the mono-ADP-ribosyltransferase ARTD10 and interaction with the ubiquitin receptor p62

Cell Communication and Signaling, 2012
Background ADP-ribosylation is a posttranslational modification catalyzed in cells by ADP-ribosyltransferases (ARTD or PARP enzymes). The ARTD family consists of 17 members. Some ARTDs modify their substrates by adding ADP-ribose in an iterative process,
Kleine Henning, Herrmann Andreas, Lamark Trond, Forst Alexandra H, Verheugd Patricia, Lüscher-Firzlaff Juliane, Lippok Barbara, Feijs Karla LH, Herzog Nicolas, Kremmer Elisabeth, Johansen Terje, Müller-Newen Gerhard, Lüscher Bernhard   +12 more
doaj   +1 more source

Small and large intestine express a truncated Dab1 isoform that assembles in cell-cell junctions and co-localizes with proteins involved in endocytosis [PDF]

, 2018
Disabled-1 (Dab1) is an essential intracellular adaptor protein in the reelin pathway. Our previous studies in mice intestine showed that Dab1 transmits the reelin signal to cytosolic signalling pathways.
Balda, María S., García Miranda, Pablo, Ilundáin Larrañeta, María Anunciación Ana, Matter, Karl, Peral Rubio, María José, Vázquez Carretero, María Dolores   +5 more
core   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Characterization of Ku70(2)-NLS as bipartite nuclear localization sequence for non-viral gene delivery. [PDF]

PLoS ONE, 2012
Several barriers have to be overcome in order to achieve gene expression in target cells, e.g. cellular uptake, endosomal release and translocation to the nucleus.
Johannes Matschke, Alexander Bohla, Christof Maucksch, Rashmi Mittal, Carsten Rudolph, Joseph Rosenecker   +5 more
doaj   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

cis-acting sequences and trans-acting factors in the localization of mRNA for mitochondrial ribosomal proteins [PDF]

, 2008
mRNA localization is a conserved post-transcriptional process crucial for a variety of systems. Although several mechanisms have been identified, emerging evidence suggests that most transcripts reach the protein functional site by moving along ...
AMORESANO, ANGELA, C. Cirulli, PIETROPAOLO, CONCETTA, PUCCI, PIETRO, RUSSO, ANNAPINA, RUSSO, GIULIA   +5 more
core   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Nuclear–Cytoplasmic Shuttling of the Usher Syndrome 1G Protein SANS Differs from Its Paralog ANKS4B

Cells
The USH1G protein SANS is a small multifunctional scaffold protein. It is involved in several different cellular processes, such as intracellular transport, in the cytoplasm, or splicing of pre-mRNA, in the cell nucleus.
Jacques S. Fritze, Felizitas F. Stiehler, Uwe Wolfrum   +2 more
doaj   +1 more source

High‐throughput, microscope‐based sorting to dissect cellular heterogeneity

Molecular Systems Biology, 2020
Microscopy is a powerful tool for characterizing complex cellular phenotypes, but linking these phenotypes to genotype or RNA expression at scale remains challenging.
Nicholas Hasle, Anthony Cooke, Sanjay Srivatsan, Heather Huang, Jason J Stephany, Zachary Krieger, Dana Jackson, Weiliang Tang, Sriram Pendyala, Raymond J Monnat, Cole Trapnell, Emily M Hatch, Douglas M Fowler   +12 more
doaj   +1 more source