Results 21 to 30 of about 208,246 (250)

Discovery of 5,5′-Methylenedi-2,3-Cresotic Acid as a Potent Inhibitor of the Chemotactic Activity of the HMGB1·CXCL12 Heterocomplex Using Virtual Screening and NMR Validation

Frontiers in Chemistry, 2020
HMGB1 is a key molecule that both triggers and sustains inflammation following infection or injury, and is involved in a large number of pathologies, including cancer.
Federica De Leo, Giacomo Quilici, Francesco De Marchis, Malisa Vittoria Mantonico, Marco Emilio Bianchi, Marco Emilio Bianchi, Giovanna Musco   +6 more
doaj   +1 more source

In-cell NMR characterization of the secondary structure populations of a disordered conformation of α-Synuclein within E. coli cells [PDF]

, 2013
α-Synuclein is a small protein strongly implicated in the pathogenesis of Parkinson’s disease and related neurodegenerative disorders. We report here the use of in-cell NMR spectroscopy to observe directly the structure and dynamics of this protein ...
A Binolfi, Anthony W. P. Fitzpatrick, AS Maltsev, AS Morar, B Fauvet, B Fauvet, BC McNulty, C Camilloni, C Huang, C Lendel, C Li, C Li, CA Waudby, CA Waudby, Carlo Camilloni, Christopher A. Waudby, Christopher M. Dobson, CO Barnes, CR Bodner, CW Bertoncini, D Eliezer, D Sakakibara, DS Biggs, DS Burz, DS Wishart, DS Wishart, E Lescop, EJ De Genst, F Chiti, F Delaglio, F Ferrage, I Bertini, IR Booth, John Christodoulou, JR Allison, K Inomata, KM Slade, KR Metz, Lisa D. Cabrita, Michele Vendruscolo, MM Dedmon, MR Cookson, P Bernadó, P Sarder, P Schanda, PH Weinreb, Piero Andrea Temussi, RC Puetter, SF Gull, T Bartels, TS Ulmer, VN Uversky, VN Uversky, W Wang, WF Vranken, WS Davidson, Z Serber, Z Serber   +57 more
core   +8 more sources

Residue-Specific High-Resolution 17O Solid-State Nuclear Magnetic Resonance of Peptides: Multidimensional Indirect 1H Detection and Magic-Angle Spinning.

Journal of Physical Chemistry Letters, 2022
Oxygen is an integral component of proteins but remains sparsely studied because its only NMR active isotope, 17O, has low sensitivity, low resolution, and large quadrupolar couplings.
I. Hung, E. Keeler, Wenping Mao, P. Gor’kov, R. Griffin, Z. Gan   +5 more
semanticscholar   +1 more source

Virtual Homonuclear Decoupling in Direct Detection Nuclear Magnetic Resonance Experiments Using Deep Neural Networks.

Journal of the American Chemical Society, 2021
Nuclear magnetic resonance (NMR) experiments are frequently complicated by the presence of homonuclear scalar couplings. For the growing body of biomolecular 13C-detected NMR methods, one-bond 13C-13C couplings significantly reduce sensitivity and ...
Gogulan Karunanithy, Harold W. Mackenzie, D. Hansen   +2 more
semanticscholar   +1 more source

Utility of methyl side chain probes for solution NMR studies of large proteins

Journal of Magnetic Resonance Open, 2023
Selective isotopic labeling of methyl side chain groups in proteins and other biomolecules, combined with advances in perdeuteration, new pulse sequences, and high field spectrometers with cryogenic probes, has revolutionized the field of solution ...
Andrew C. McShan
doaj   +1 more source

Site-Specific Introduction of Alanines for the Nuclear Magnetic Resonance Investigation of Low-Complexity Regions and Large Biomolecular Assemblies

ACS Chemical Biology, 2023
Nuclear magnetic resonance (NMR) studies of large biomolecular machines and highly repetitive proteins remain challenging due to the difficulty of assigning frequencies to individual nuclei. Here, we present an efficient strategy to address this challenge by engineering a Pyrococcus horikoshii tRNA/alanyl-tRNA synthetase pair that enables the ...
Elena-Real, Carlos, Urbanek, Annika, Imbert, Lionel, Morató, Anna, Fournet, Aurélie, Allemand, Frédéric, Sibille, Nathalie, Boisbouvier, Jérôme, Bernadó, Pau   +8 more
openaire   +4 more sources

Synthesis of lanthanide tag and experimental studies on paramagnetically induced residual dipolar couplings

BMC Chemistry, 2022
Nuclear Magnetic Resonance (NMR) spectroscopy is an indispensable technique for the structure elucidation of molecules and determination of their characteristic interactions.
Ali Yassin, Bilal Nehmeh, Sally El Kantar, Yara Al Kazzaz, Elias Akoury   +4 more
doaj   +1 more source

Integrating a prospective pilot trial and patient-derived xenografts to trace metabolic changes associated with acute myeloid leukemia

Journal of Hematology & Oncology, 2016
Despite the considerable progress in understanding the molecular bases of acute myeloid leukemia (AML), new tools to link disease biology to the unpredictable patient clinical course are still needed.
Matteo G. Carrabba, Laurette Tavel, Giacomo Oliveira, Alessandra Forcina, Giacomo Quilici, Francesca Nardelli, Cristina Tresoldi, Alessandro Ambrosi, Fabio Ciceri, Massimo Bernardi, Luca Vago, Giovanna Musco   +11 more
doaj   +1 more source

¹⁷O NMR Spectroscopy: A Novel Probe for Characterizing Protein Structure and Folding

Biology, 2021
Oxygen is a key atom that maintains biomolecular structures, regulates various physiological processes, and mediates various biomolecular interactions. Oxygen-17 (17O), therefore, has been proposed as a useful probe that can provide detailed information ...
Srinivasan Muniyappan, Yuxi Lin, Young-Ho Lee, Jin Hae Kim   +3 more
doaj   +1 more source

Model-Free or Not?

Frontiers in Molecular Biosciences, 2021
Relaxation in nuclear magnetic resonance is a powerful method for obtaining spatially resolved, timescale-specific dynamics information about molecular systems. However, dynamics in biomolecular systems are generally too complex to be fully characterized
Kai Zumpfe, Albert A. Smith
doaj   +1 more source