Nuclear Polyadenylate-Binding Protein [PDF]
Molecular and Cellular Biology, 1985 Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously ...
A B, Sachs, R D, Kornberg
openaire +2 more sources
Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins
Scientific Reports, 2015 Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and ...
Yeong Ju Shin +11 more
semanticscholar +1 more source
Heat shock protein 60 in vascular biology [PDF]
Scripta MedicaThe response of heat shock proteins (HSPs) to stress-induced stimuli is now well documented and understood. Specific HSPs like HSP70 play an important role in vascular diseases like atherosclerosis and hypertension. However, the involvement of other HSPs
Hedley Thomas E. +3 more
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The SUMO Ligase Protein Inhibitor of Activated STAT 1 (PIAS1) is a constituent PML-NB protein that contributes to the intrinsic antiviral immune response to herpes simplex virus 1 (HSV-1) [PDF]
, 2016 Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukaemia (PML)-nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting
Boutell, Chris +7 more
core +1 more source
Phylogenetic analysis of the SAP30 family of transcriptional regulators reveals functional divergence in the domain that binds the nuclear matrix [PDF]
, 2009 Background: Deacetylation of histones plays a fundamental role in gene silencing, and this is mediated by a corepressor complex containing Sin3 as an essential scaffold protein. In this report we examine the evolution of two proteins in this complex, the
Heinonen, TYK +3 more
core +3 more sources
A Muscle Hybrid Promoter as a Novel Tool for Gene Therapy
Molecular Therapy: Methods & Clinical Development, 2019 Gene therapy is a promising strategy to cure rare diseases. The lack of regulatory sequences ensuring specific and robust expression in skeletal and cardiac muscle is a substantial limitation of gene therapy efficiency targeting the muscle tissue.
Katarzyna Piekarowicz +7 more
doaj +1 more source
Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins. [PDF]
PLoS Pathogens, 2015 The paramyxovirus matrix (M) protein is a molecular scaffold required for viral morphogenesis and budding at the plasma membrane. Transient nuclear residence of some M proteins hints at non-structural roles.
Mickey Pentecost +10 more
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Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans [PDF]
, 2018 C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms.
Mata Cabana, Alejandro +3 more
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Effect of nitric oxide on gene transcription – S-nitrosylation of nuclear proteins
Frontiers in Plant Science, 2013 Nitric oxide (NO) plays an important role in many different physiological processes in plants. It mainly acts by post-translationally modifying proteins.
Alexander Mengel +3 more
semanticscholar +1 more source
Cells, 2021 Specific nuclear sub-compartments that are regions of fundamental processes such as gene expression or DNA repair, contain phosphoinositides (PIPs). PIPs thus potentially represent signals for the localization of specific proteins into different nuclear ...
Martin Sztacho +5 more
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