Nuclear Polyadenylate-Binding Protein [PDF]
Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously ...
Roger D. Kornberg, Alan B. Sachs
openaire +3 more sources
Insertion of proteins into the inner membrane of mitochondria: the role of the Oxa1 complex [PDF]
The inner mitochondrial membrane harbors a large number of proteins that display a wide range of topological arrangements. The majority of these proteins are encoded in the cell\u27s nucleus, but a few polytopic proteins, all subunits of respiratory ...
Stuart, Rosemary A.
core +2 more sources
NcPred for accurate nuclear protein prediction using n-mer statistics with various classification algorithms [PDF]
Prediction of nuclear proteins is one of the major challenges in genome annotation. A method, NcPred is described, for predicting nuclear proteins with higher accuracy exploiting n-mer statistics with different classification algorithms namely ...
A. Ganesh+14 more
core +3 more sources
An optimization strategy on prion AGAAAAGA amyloid fibril molecular modeling [PDF]
X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy are two powerful tools to determine the protein 3D structure. However, not all proteins can be successfully crystallized, particularly for membrane proteins. Although NMR spectroscopy is indeed very powerful in determining the 3D structures of membrane proteins, same as X-ray ...
arxiv +2 more sources
Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage [PDF]
The plastids of ecologically and economically important algae from phyla such as stramenopiles, dinoflagellates and cryptophytes were acquired via a secondary endosymbiosis and are surrounded by three or four membranes.
Allen+62 more
core +2 more sources
Nuclear transport proteins: structure, function and disease relevance
Proper subcellular localization is crucial for the functioning of biomacromolecules, including proteins and RNAs. Nuclear transport is a fundamental cellular process that regulates the localization of many macromolecules within the nuclear or cytoplasmic
Yang Yang+5 more
doaj +1 more source
Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior [PDF]
While much has been learned in recent years about the movement of soluble transport factors across the nuclear pore complex (NPC), comparatively little is known about intranuclear trafficking. We isolated the previously identified Saccharomyces protein Mlp1p (myosin-like protein) by an assay designed to find nuclear envelope (NE) associated proteins ...
Günter Blobel+2 more
openaire +3 more sources
The SUMO Ligase Protein Inhibitor of Activated STAT 1 (PIAS1) is a constituent PML-NB protein that contributes to the intrinsic antiviral immune response to herpes simplex virus 1 (HSV-1) [PDF]
Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukaemia (PML)-nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting
Boutell, Chris+7 more
core +1 more source
Nuclear Photosynthetic Gene Expression Is Synergistically Modulated by Rates of Protein Synthesis in Chloroplasts and Mitochondria [PDF]
Arabidopsis thaliana mutants prors1-1 and -2 were identified on the basis of a decrease in effective photosystem II quantum yield. Mutations were localized to the 5'-untranslated region of the nuclear gene PROLYL-tRNA SYNTHETASE1 (PRORS1), which acts in ...
Bhushan, Shashi+7 more
core +2 more sources
The intriguing plant nuclear lamina
The nuclear lamina is a complex protein mesh attached to the inner nuclear membrane, which is also associated with nuclear pore complexes. It provides mechanical support to the nucleus and nuclear envelope, and as well as facilitating the connection of ...
Malgorzata eCiska+1 more
doaj +1 more source