Comparison of in vitro fertilization and nuclear transfer techniques in the production of buffalo pre-implantation embryos [PDF]
This study was conducted to evaluate the timing of cleavage and blastocoel formation of in vitro fertilized and nuclear transfer embryos and to compare the efficiency of in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) techniques in ...
L.C. Cruz +5 more
doaj +3 more sources
An indirect technique in nuclear astrophysics: alpha-cluster transfer reaction [PDF]
Helium(4He, or α)is the second most abundant element in the observable Universe. The α-particle induced reactions such as(α, γ), (α, n) and (α, p) play a crucial role in nuclear astrophysics, especially for understanding stellar heliumburning. Because of the strong Coulomb repulsion, it is greatly hindered to directly measure the cross sections for ...
Y. P. Shen, Bing Guo, Weiping Liu
openalex +3 more sources
Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer [PDF]
Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT.
Min Jee Park +5 more
openalex +3 more sources
Comparative analysis of different nuclear transfer techniques to prevent the transmission of mitochondrial DNA variants [PDF]
Abstract Prevention of mitochondrial DNA (mtDNA) diseases may currently be possible using germline nuclear transfer (NT). However, scientific evidence to compare efficiency of different NT techniques to overcome mtDNA diseases is lacking.
Miao Tang +13 more
+6 more sources
Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique [PDF]
Los objetivos de este trabajo fueron los de producir embriones de pudu, obtenidos por la transferencia de nucleos de fibroblastos de la oreja de pudu en ovocitos de un rumiante domesticos que es el bovino. Para posteriormente en un trabajo futuro proceder a la transferencia de embriones de pudu, al utero de hembras receptoras sincronizadas de otra ...
Felipe Venegas +9 more
openalex +4 more sources
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells [PDF]
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In canines, however, it is difficult to obtain large quantities of oocytes because each donor produces a limited number of mature oocytes in vivo.
Satoshi Sugimura, Eimei Sato
openalex +2 more sources
マウス2細胞期(野生色)胚の片側割球の核を2あるいは8細胞期(アルビノ)胚の核と置換してキメラ2細胞期胚を作出し,異なる発生ステージ間の胚からキメラマウスの作出を試みた。また,産子の毛色パターンから移植核の発生動態についても考察した。得られた結果は次ぎの通りである。1)2細胞期胚の片側割球の核を2あるいは8細胞期胚の核と置換したいずれの場合,キメラ胚の胚盤胞並びに移植後の産子の発生率は,それぞれ81%-90%および59-76%といずれも高い値であった。2)2細胞期同士で核置換したキメラ2細胞期胚からは,高率にキメラ個体がえられた。しかし,8細胞期胚の核で置換するとキメラ出現率は減少し,野生色個体は逆に増加した。3)したがって,8細胞期胚の核を持つ2細胞期片側割球由来の細胞は ...
Tomohiro Kono +3 more
openalex +3 more sources
Due to Thailand's nuclear energy public acceptance problem, the understanding of nuclear energy public perception was the key factor affecting to re-consideration of the nuclear energy program. Thailand Institute of Nuclear Technology and its alliances together developed the classification model for the nuclear energy public perception from the big ...
Wasin Vechgama +2 more
openalex +3 more sources
Nuclear transfer technique affects mRNA abundance, developmental competence and cell fate of the reconstituted sheep oocytes [PDF]
The effect of technical steps of somatic cell nuclear transfer (SCNT) on different aspects of cloned embryo development was investigated in sheep.In vitro-matured oocytes were enucleated in the presence or absence of zona and reconstituted by three different SCNT techniques: conventional zona-intact (ZI-NT), standard zona-free (ZF-NT) and ...
Fariba Moulavi +7 more
openalex +3 more sources
Application of Machine Learning to Discriminate Photoreceptor Cell Species in Xenotransplanted Chimeric Retinas [PDF]
Photoreceptor transplantation is being studied to restore visual function in retinal diseases causing blindness, including age‐related macular degeneration, hereditary eye diseases, and traumatic retinopathy.
Kang V. Li +14 more
doaj +2 more sources

