Results 1 to 10 of about 519,067 (307)

Comparison of in vitro fertilization and nuclear transfer techniques in the production of buffalo pre-implantation embryos [PDF]

Italian Journal of Animal Science, 2010
This study was conducted to evaluate the timing of cleavage and blastocoel formation of in vitro fertilized and nuclear transfer embryos and to compare the efficiency of in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) techniques in ...
L.C. Cruz, F.V. Mamuad, R.V. de Vera, D.H. Duran, E.P. Atabay, E.C. Atabay   +5 more
doaj   +4 more sources

An indirect technique in nuclear astrophysics: alpha-cluster transfer reaction [PDF]

EPJ Web of Conferences, 2022
Helium(4He, or α)is the second most abundant element in the observable Universe. The α-particle induced reactions such as(α, γ), (α, n) and (α, p) play a crucial role in nuclear astrophysics, especially for understanding stellar heliumburning. Because of the strong Coulomb repulsion, it is greatly hindered to directly measure the cross sections for ...
Shen Yang-Ping, Guo Bing, Liu Wei-Ping
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Comparative analysis of different nuclear transfer techniques to prevent the transmission of mitochondrial DNA variants [PDF]

Molecular Human Reproduction, 2019
Abstract Prevention of mitochondrial DNA (mtDNA) diseases may currently be possible using germline nuclear transfer (NT). However, scientific evidence to compare efficiency of different NT techniques to overcome mtDNA diseases is lacking.
Tang, M, Guggilla, R R, Gansemans, Y, Jeught, M Van der, Boel, A, Popovic, M, Stamatiadis, P, Ferrer-Buitrago, M, Thys, V, Coster, R Van, Deforce, D, Sutter, P De, Nieuwerburgh, F Van, Heindryckx, B   +13 more
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Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique [PDF]

International Journal of Morphology, 2006
Los objetivos de este trabajo fueron los de producir embriones de pudu, obtenidos por la transferencia de nucleos de fibroblastos de la oreja de pudu en ovocitos de un rumiante domesticos que es el bovino. Para posteriormente en un trabajo futuro proceder a la transferencia de embriones de pudu, al utero de hembras receptoras sincronizadas de otra ...
Venegas, Felipe, Guillomot, Michel, Vignon, Xavier, Servely, Jean Luc, Audouard, Chistophe, Montiel, Enrique, Le Bourhis, Daniel, Perón, Sandrine, Soto, Pilar, Rojas, Mariana   +9 more
openaire   +5 more sources

Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer [PDF]

Cellular Reprogramming, 2015
Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT.
Chang Jin Jeong, Se Pill Park, Min Jee Park, Eun-Young Kim, Jun Beom Lee, Seung-Eun Lee   +5 more
openaire   +4 more sources

Nuclear transfer technique affects mRNA abundance, developmental competence and cell fate of the reconstituted sheep oocytes [PDF]

REPRODUCTION, 2013
The effect of technical steps of somatic cell nuclear transfer (SCNT) on different aspects of cloned embryo development was investigated in sheep.In vitro-matured oocytes were enucleated in the presence or absence of zona and reconstituted by three different SCNT techniques: conventional zona-intact (ZI-NT), standard zona-free (ZF-NT) and ...
F. Moulavi, Mohsen Forouzanfar, P. Abedi, S.M. Hosseini, V. Asgari, S. Ostadhosseini, Mehdi Hajian, Mohammad Hossein Nasr-Esfahani   +7 more
openaire   +4 more sources

Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells [PDF]

Asian-Australasian Journal of Animal Sciences, 2010
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In canines, however, it is difficult to obtain large quantities of oocytes because each donor produces a limited number of mature oocytes in vivo.
Satoshi Sugimura, Eimei Sato
openaire   +3 more sources

Development of novel intracytoplasmic sperm injection and somatic cell nuclear transfer techniques for animal reproduction [PDF]

Animal Science Journal, 2010
ABSTRACTAnimal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have
Teruhiko Wakayama
openaire   +4 more sources

Development of chimaeras produced from highly asynchronous mouse embryos using nuclear transfer technique.

The Japanese journal of animal reproduction, 1989
マウス2細胞期(野生色)胚の片側割球の核を2あるいは8細胞期(アルビノ)胚の核と置換してキメラ2細胞期胚を作出し,異なる発生ステージ間の胚からキメラマウスの作出を試みた。また,産子の毛色パターンから移植核の発生動態についても考察した。得られた結果は次ぎの通りである。1)2細胞期胚の片側割球の核を2あるいは8細胞期胚の核と置換したいずれの場合,キメラ胚の胚盤胞並びに移植後の産子の発生率は,それぞれ81%-90%および59-76%といずれも高い値であった。2)2細胞期同士で核置換したキメラ2細胞期胚からは,高率にキメラ個体がえられた。しかし,8細胞期胚の核で置換するとキメラ出現率は減少し,野生色個体は逆に増加した。3)したがって,8細胞期胚の核を持つ2細胞期片側割球由来の細胞は ...
Tomohiro Kono, Tatsuo Nakahara, Tadao Watanabe, Yukio Tsunoda   +3 more
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2009–2022 Thailand public perception analysis of nuclear energy on social media using deep transfer learning technique

Nuclear Engineering and Technology, 2023
Due to Thailand's nuclear energy public acceptance problem, the understanding of nuclear energy public perception was the key factor affecting to re-consideration of the nuclear energy program. Thailand Institute of Nuclear Technology and its alliances together developed the classification model for the nuclear energy public perception from the big ...
Wasin Vechgama, Watcha Sasawattakul, Kampanart Silva   +2 more
openaire   +3 more sources